Cucsa.322620 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.322620
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionFlavanone 3-hydroxylase
Locationscaffold03080 : 2324085 .. 2326058 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCAACAATGACTTTTTCTCTCCCACATCATCCCACCATTCTGGCCCCGGAATTCGTCCGCCACCATGACGACCGTCCAAAGGTCCCTTACAACCAATTCAGCGATGAAATACCCATCATCTCCCTTTCCGGAATTGACGACAACGGCGAACGCAGGGCCATGATTCGCCACAGCATTGTTGAAGCTTGCGAGGAATGGGGTATCTTCCAGGTGGTGGACCATGGCGTCGATTCCGAGATTGTTTCCGATATGACTCGTCTGGCTGGAGAGTTCTTTGGTCTTCCGACGGAGGAGAAACTCCGTTTCGATATGTCCGGCGGTAAAAGGGGGGgATTCAACGTCTCTAGTCATCTCAAGGTCTCTCTCTCTATATATATAAAGATGAACTAAGTATATATATAACTTTATGTACAATGAAAAATAAGAACAAACTTTGATTAAACACAATCTAAGTACAGTTTAAATTATTCAGCTTTTTCATATTTGAAATTAAACAAAAACACATCTTTTAATCACTCTTTGAATGATTAAAGAGAATGTTTTACATATGTATAGAAAATATCAAACTATTTAGAAGAAATAGCAAAGAAATCACTTGTGGGTTTCTATCAATCTCTATGATAAGACTTTTATCCATGGTTTTtCTTTTTTtCAAAATATAAAAAATTGTAATGAAACATGAAAAGAGAATTTTATAATTGTTTTTAATGCTTTATAATTACGATATAAATGTGTTAAGAATTACTAAATGGATTTGGTCAACTTAATAGCAACAACCAAAAAaGTATATTAAGCTATTGAATTACAAATCCTAAAACTTTAATTAACCTAATTATTTAAGGTGGGATTTGAATATAGTTTTGATTTCGTCAATGAGAATTAATTTTTGTTTACTCATAAACTCTAAGCAATGATCTTTTTCCTATACAAAAACTGCATTAACGAACGAAGGCATTATCATGTGTACTCTTAAGTTGAGATTGTTTACATTACAGGGAGAGATTGTTAAAGATTGGCGTGAGTTAGTAACATTCTTCACATACCCAATTGAGAAAAGAGACTACTCTTTTTGGCCGGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGTGAGAAGTTGATGGGGTTGGCTTGTAAGCTTCTGGAGGTTTTATCGGAAGCTATGGGTTTGGAGGAGGAGGCCTTGAAAAAGGCTTGTGTGGACTTCGATCAGAAGATTGTTGTGAATTTCTACCCCAAATGTCCTGAGCCAGACCTCACCTTGGGGCTAAAGCGCCATACTGATCCTGGAACCATAACCTTGTTGCTACAGGACCAAGTTGGTGGCCTTCAGGTCACCAAGGATGGTGGACGGACGTGGATTACTGTCCAACCTGTTCAAGGCGCTTTCGTTGTCAACTTAGGTGATCATGGTCATGTAAGTCTCTGCTTAATTTAGTTTTGAAATCAACTTTCAAGTTTGAATTTAATGTGTGGATGATTGAATTTTGTTGGAATAGTATGTTAGCAATGGGAGGTTCAAGAGTGGTGATCACCAAGCAGTAGTGAACTCAAAGTCAAGCAGGATATCAATAGCCACGTTTCAGTACCCTTCACAGGAATCAATTGTGTATCCATTGAAGATTAGAGATGGGGAGAAGCCAATTCTAGAAAAACCCATCACTTTCTCTGAGATGTATCACATGAAGATGAAGAATGATATTGAGTTTGCAAGGCTTAAGAAGCAAACCCAAAAACAAGCTCAAGACTTTGTTGCTTGATATCCCCAATCGCTAAGATTCATCATCTTTATATATGCTTTCAGCTTTTAAATAAAAAAaTGTAATTTGGATCTATCAACTTTGGTATTTACTTTAATTAAATAGTCTAAACAATCTAAAATTAGGAAAAACAGTATTTGATTTTAGTATGTTTAGATTCTGTGTGCTTTTTTTtCAGACCACGATTATCCTTCAATAATGAAAAG

mRNA sequence

ACCAACAATGACTTTTTCTCTCCCACATCATCCCACCATTCTGGCCCCGGAATTCGTCCGCCACCATGACGACCGTCCAAAGGTCCCTTACAACCAATTCAGCGATGAAATACCCATCATCTCCCTTTCCGGAATTGACGACAACGGCGAACGCAGGGCCATGATTCGCCACAGCATTGTTGAAGCTTGCGAGGAATGGGGTATCTTCCAGGTGGTGGACCATGGCGTCGATTCCGAGATTGTTTCCGATATGACTCGTCTGGCTGGAGAGTTCTTTGGTCTTCCGACGGAGGAGAAACTCCGTTTCGATATGTCCGGCGGTAAAAGGGGGGGATTCAACGTCTCTAGTCATCTCAAGGGAGAGATTGTTAAAGATTGGCGTGAGTTAGTAACATTCTTCACATACCCAATTGAGAAAAGAGACTACTCTTTTTGGCCGGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGTGAGAAGTTGATGGGGTTGGCTTGTAAGCTTCTGGAGGTTTTATCGGAAGCTATGGGTTTGGAGGAGGAGGCCTTGAAAAAGGCTTGTGTGGACTTCGATCAGAAGATTGTTGTGAATTTCTACCCCAAATGTCCTGAGCCAGACCTCACCTTGGGGCTAAAGCGCCATACTGATCCTGGAACCATAACCTTGTTGCTACAGGACCAAGTTGGTGGCCTTCAGGTCACCAAGGATGGTGGACGGACGTGGATTACTGTCCAACCTGTTCAAGGCGCTTTCGTTGTCAACTTAGGTGATCATGGTCATTATGTTAGCAATGGGAGGTTCAAGAGTGGTGATCACCAAGCAGTAGTGAACTCAAAGTCAAGCAGGATATCAATAGCCACGTTTCAGTACCCTTCACAGGAATCAATTGTGTATCCATTGAAGATTAGAGATGGGGAGAAGCCAATTCTAGAAAAACCCATCACTTTCTCTGAGATGTATCACATGAAGATGAAGAATGATATTGAGTTTGCAAGGCTTAAGAAGCAAACCCAAAAACAAGCTCAAGACTTTGTTGCTTGATATCCCCAATCGCTAAGATTCATCATCTTTATATATGCTTTCAGCTTTTAAATAAAAAAATGTAATTTGGATCTATCAACTTTGGTATTTACTTTAATTAAATAGTCTAAACAATCTAAAATTAGGAAAAACAGTATTTGATTTTAGTATGTTTAGATTCTGTGTGCTTTTTTTTCAGACCACGATTATCCTTCAATAATGAAAAG

Coding sequence (CDS)

ATGACTTTTTCTCTCCCACATCATCCCACCATTCTGGCCCCGGAATTCGTCCGCCACCATGACGACCGTCCAAAGGTCCCTTACAACCAATTCAGCGATGAAATACCCATCATCTCCCTTTCCGGAATTGACGACAACGGCGAACGCAGGGCCATGATTCGCCACAGCATTGTTGAAGCTTGCGAGGAATGGGGTATCTTCCAGGTGGTGGACCATGGCGTCGATTCCGAGATTGTTTCCGATATGACTCGTCTGGCTGGAGAGTTCTTTGGTCTTCCGACGGAGGAGAAACTCCGTTTCGATATGTCCGGCGGTAAAAGGGGGGgATTCAACGTCTCTAGTCATCTCAAGGGAGAGATTGTTAAAGATTGGCGTGAGTTAGTAACATTCTTCACATACCCAATTGAGAAAAGAGACTACTCTTTTTGGCCGGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGTGAGAAGTTGATGGGGTTGGCTTGTAAGCTTCTGGAGGTTTTATCGGAAGCTATGGGTTTGGAGGAGGAGGCCTTGAAAAAGGCTTGTGTGGACTTCGATCAGAAGATTGTTGTGAATTTCTACCCCAAATGTCCTGAGCCAGACCTCACCTTGGGGCTAAAGCGCCATACTGATCCTGGAACCATAACCTTGTTGCTACAGGACCAAGTTGGTGGCCTTCAGGTCACCAAGGATGGTGGACGGACGTGGATTACTGTCCAACCTGTTCAAGGCGCTTTCGTTGTCAACTTAGGTGATCATGGTCATTATGTTAGCAATGGGAGGTTCAAGAGTGGTGATCACCAAGCAGTAGTGAACTCAAAGTCAAGCAGGATATCAATAGCCACGTTTCAGTACCCTTCACAGGAATCAATTGTGTATCCATTGAAGATTAGAGATGGGGAGAAGCCAATTCTAGAAAAACCCATCACTTTCTCTGAGATGTATCACATGAAGATGAAGAATGATATTGAGTTTGCAAGGCTTAAGAAGCAAACCCAAAAACAAGCTCAAGACTTTGTTGCTTGA

Protein sequence

MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA*
Homology
[Close]
BLAST of Cucsa.322620 vs. Swiss-Prot
Match: FL3H_PETHY (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida GN=AN3 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.1e-147
Identity = 248/329 (75.38%), Postives = 285/329 (86.63%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L   F+R  D+RPKV YNQFS+EIPIISL GIDD   +RA I   IV+ACE+WG+FQVVD
Sbjct: 18  LQTSFIRDEDERPKVAYNQFSNEIPIISLEGIDDETGKRAEICDKIVKACEDWGVFQVVD 77

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD+E++S MT  A EFF LP EEKLRFDMSGGK+GGF VSSHL+GE+V+DWRE+VT+F
Sbjct: 78  HGVDAEVISQMTTFAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYF 137

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP   RDYS WPDKPEGW A T +YSEKLM LACKLL+VLSEAMGLE+EAL KACVD D
Sbjct: 138 SYPTRARDYSRWPDKPEGWIAVTQKYSEKLMELACKLLDVLSEAMGLEKEALTKACVDMD 197

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QK+VVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQ TKD G+TWITVQPV+GAF
Sbjct: 198 QKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAF 257

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGH++SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+IVYPLKIR+GEK I++
Sbjct: 258 VVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKSIMD 317

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQ 341
           +PITF+EMY  KM  D+E ARLKKQ ++Q
Sbjct: 318 EPITFAEMYRRKMSKDLELARLKKQAKEQ 346

[Close]
BLAST of Cucsa.322620 vs. Swiss-Prot
Match: FL3H_MATIN (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana GN=FHT PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 7.5e-146
Identity = 245/329 (74.47%), Postives = 284/329 (86.32%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L  +FVR  D+RPKV YN+FSDEIP+ISL+GIDD   +R  I   IVEACE WGIFQVVD
Sbjct: 14  LNSKFVRDEDERPKVAYNEFSDEIPVISLAGIDDVDGKRGEICREIVEACENWGIFQVVD 73

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD+ +V+DMTRLA +FF LP EEKLRFDMSGGK+GGF VSSHL+GE V+DWRE+VT+F
Sbjct: 74  HGVDTSLVADMTRLARDFFALPPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYF 133

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP+  RDYS WPDKP+GWA  T EYSEKLMGLACKLLEVLSEAMGLE+E+L  ACVD D
Sbjct: 134 SYPVRNRDYSRWPDKPQGWAKVTEEYSEKLMGLACKLLEVLSEAMGLEKESLTNACVDMD 193

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ T+D G TWITVQPV+GAF
Sbjct: 194 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDGNTWITVQPVEGAF 253

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGH++SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+ VYPLK+R+GEK I+E
Sbjct: 254 VVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPLKVREGEKAIME 313

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQ 341
           +PITF+EMY  KM  D+E ARLKK  +++
Sbjct: 314 EPITFAEMYKRKMGRDLELARLKKLAKEE 342

[Close]
BLAST of Cucsa.322620 vs. Swiss-Prot
Match: FL3H_MALDO (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.4e-144
Identity = 246/337 (73.00%), Postives = 286/337 (84.87%), Query Frame = 1 

Query: 8   HPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIF 67
           H   L  +FVR  D+RPKV YN FS+EIPIISL+GID+   RR  I   IV ACE+WGIF
Sbjct: 12  HEKTLQQKFVRDEDERPKVAYNDFSNEIPIISLAGIDEVEGRRGEICKKIVAACEDWGIF 71

Query: 68  QVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWREL 127
           Q+VDHGVD+E++S+MT LA EFF LP+EEKLRFDMSGGK+GGF VSSHL+GE V+DWRE+
Sbjct: 72  QIVDHGVDAELISEMTGLAREFFALPSEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREI 131

Query: 128 VTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKAC 187
           VT+F+YPI  RDYS WPDKPE W   T +YS++LMGLACKLL VLSEAMGL+ EAL KAC
Sbjct: 132 VTYFSYPIRHRDYSRWPDKPEAWREVTKKYSDELMGLACKLLGVLSEAMGLDTEALTKAC 191

Query: 188 VDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPV 247
           VD DQK+VVNFYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ T+D G+TWITVQPV
Sbjct: 192 VDMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDGKTWITVQPV 251

Query: 248 QGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEK 307
           +GAFVVNLGDHGH +SNGRFK+ DHQAVVNS SSR+SIATFQ P+QE+IVYPL +R+GEK
Sbjct: 252 EGAFVVNLGDHGHLLSNGRFKNADHQAVVNSNSSRLSIATFQNPAQEAIVYPLSVREGEK 311

Query: 308 PILEKPITFSEMYHMKMKNDIEFARLKK-QTQKQAQD 344
           PILE PIT++EMY  KM  D+E ARLKK   ++Q+QD
Sbjct: 312 PILEAPITYTEMYKKKMSKDLELARLKKLAKEQQSQD 348

[Close]
BLAST of Cucsa.322620 vs. Swiss-Prot
Match: FL3H_ARATH (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana GN=F3H PE=1 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 4.1e-144
Identity = 242/329 (73.56%), Postives = 282/329 (85.71%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L  +FVR  D+RPKV YN FSDEIP+ISL+GIDD   +R  I   IVEACE WGIFQVVD
Sbjct: 15  LNSKFVRDEDERPKVAYNVFSDEIPVISLAGIDDVDGKRGEICRQIVEACENWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD+ +V+DMTRLA +FF LP E+KLRFDMSGGK+GGF VSSHL+GE V+DWRE+VT+F
Sbjct: 75  HGVDTNLVADMTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP+  RDYS WPDKPEGW   T EYSE+LM LACKLLEVLSEAMGLE+E+L  ACVD D
Sbjct: 135 SYPVRNRDYSRWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ T+D G+TWITVQPV+GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGH++SNGRFK+ DHQAVVNS SSR+SIATFQ P+ ++ VYPLK+R+GEK ILE
Sbjct: 255 VVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQ 341
           +PITF+EMY  KM  D+E ARLKK  +++
Sbjct: 315 EPITFAEMYKRKMGRDLELARLKKLAKEE 343

[Close]
BLAST of Cucsa.322620 vs. Swiss-Prot
Match: FL3H_PETCR (Flavanone 3-dioxygenase OS=Petroselinum crispum GN=FHT PE=1 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 4.6e-143
Identity = 237/330 (71.82%), Postives = 287/330 (86.97%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNG-ERRAMIRHSIVEACEEWGIFQVV 71
           L  +FVR  D+RPK+ YN+FSDEIP+ISL+GIDD+  ++R+ I   IVEACE+WGIFQVV
Sbjct: 15  LNSKFVRDEDERPKIAYNKFSDEIPVISLAGIDDDSVDKRSQICRKIVEACEDWGIFQVV 74

Query: 72  DHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTF 131
           DHG+D +++S+MTRLA +FF LP EEKLRFDM+GGK+GGF VSSHL+GE V+DWRE+VT+
Sbjct: 75  DHGIDIDLISEMTRLARQFFALPAEEKLRFDMTGGKKGGFIVSSHLQGEAVQDWREIVTY 134

Query: 132 FTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDF 191
           F+YPI+ RDYS WPDKPEGW + T  YS++LM LACKLLEVLSEAMGLE+E L KACVD 
Sbjct: 135 FSYPIQARDYSRWPDKPEGWRSITEMYSDELMALACKLLEVLSEAMGLEKEGLTKACVDM 194

Query: 192 DQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGA 251
           DQK++VN+YPKCP+P+LTLGLKRHTDPGTITLLLQDQVGGLQ T+DGG+TWITVQPV+GA
Sbjct: 195 DQKVIVNYYPKCPQPNLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGA 254

Query: 252 FVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPIL 311
           FVVNLGDHGHY+SNGRFK+ DHQAVVNS SSR+SIATFQ P+  + VYPLKIR+GEK ++
Sbjct: 255 FVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRMSIATFQNPAPNATVYPLKIREGEKAVM 314

Query: 312 EKPITFSEMYHMKMKNDIEFARLKKQTQKQ 341
           E+PITF+EMY  KM  DIE A LKK  +++
Sbjct: 315 EEPITFAEMYKRKMSRDIEMATLKKLAKEK 344

[Close]
BLAST of Cucsa.322620 vs. TrEMBL
Match: A0A0A0L678_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002800 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 8.8e-202
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 1 

Query: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60
           MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA
Sbjct: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60

Query: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120
           CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI
Sbjct: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120

Query: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180
           VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE
Sbjct: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180

Query: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240
           EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT
Sbjct: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240

Query: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300
           WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL
Sbjct: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300

Query: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 347
           KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA
Sbjct: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 346

[Close]
BLAST of Cucsa.322620 vs. TrEMBL
Match: E5RDD3_CUCME (Flavanone 3-hydroxylase OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.3e-184
Identity = 316/346 (91.33%), Postives = 331/346 (95.66%), Query Frame = 1 

Query: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60
           M  SLPHHPTILAPEF+RH DDRPKVPY+QFSDEIPIISL+GID +GERRA+IR SIVEA
Sbjct: 1   MPSSLPHHPTILAPEFLRHPDDRPKVPYDQFSDEIPIISLAGIDVDGERRAVIRRSIVEA 60

Query: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120
           CEEWGIFQVVDHGVDSEIVSDMTRLAG FF LPTEEKLRFDMSGGK+GGFN+SSHL+GE+
Sbjct: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGHFFALPTEEKLRFDMSGGKKGGFNISSHLQGEV 120

Query: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180
           VKDWRELVTFF+YPIEKRDYS WP+KPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLE+
Sbjct: 121 VKDWRELVTFFSYPIEKRDYSLWPNKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEK 180

Query: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240
            AL+K CVD DQKIVVNFYPKCPEPD TLGLKRHTDPGTITLLLQDQVGGLQ TKDGGRT
Sbjct: 181 GALRKTCVDLDQKIVVNFYPKCPEPDFTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRT 240

Query: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300
           WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSR+SIATFQYPSQESIVYPL
Sbjct: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRMSIATFQYPSQESIVYPL 300

Query: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 347
           KIRDGEKPILEKPITFSEMYHMKMKNDIE ARLKKQ Q+QAQDFVA
Sbjct: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIESARLKKQAQQQAQDFVA 346

[Close]
BLAST of Cucsa.322620 vs. TrEMBL
Match: A0A067FUZ4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017946mg PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 8.7e-149
Identity = 252/332 (75.90%), Postives = 291/332 (87.65%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L P FVR  D+RPKV YN+FS+EIP+ISL+GIDD G +RA I   IVEACE+WGIFQVVD
Sbjct: 15  LNPSFVRFQDERPKVAYNEFSNEIPVISLAGIDDVGGKRAEICKKIVEACEDWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD++++SDMTRLA EFF LP EEKL+FDMSGGK+GGF VSSHL+GE+VKDWRE+VT+F
Sbjct: 75  HGVDAKLISDMTRLATEFFALPPEEKLKFDMSGGKKGGFIVSSHLQGEVVKDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           ++P + RDYS WPDKPEGW   T EYS+KLMG+ACKLLEVLSEAMGLE+EAL KACVD D
Sbjct: 135 SFPKQSRDYSRWPDKPEGWMEVTKEYSDKLMGVACKLLEVLSEAMGLEKEALTKACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ TKD G+TWITVQP++GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPIEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGHY+SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+ VYPLKIR+GEKP+LE
Sbjct: 255 VVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPLKIREGEKPVLE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQAQD 344
           +PI FSEMY  KM  D+E ARLKK   ++ QD
Sbjct: 315 EPIPFSEMYRRKMSKDLELARLKKLANERHQD 346

[Close]
BLAST of Cucsa.322620 vs. TrEMBL
Match: V4UHD8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025931mg PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 8.7e-149
Identity = 252/332 (75.90%), Postives = 291/332 (87.65%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L P FVR  D+RPKV YN+FS+EIP+ISL+GIDD G +RA I   IVEACE+WGIFQVVD
Sbjct: 15  LNPSFVRFQDERPKVAYNEFSNEIPVISLAGIDDVGGKRAEICKKIVEACEDWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD++++SDMTRLA EFF LP EEKL+FDMSGGK+GGF VSSHL+GE+VKDWRE+VT+F
Sbjct: 75  HGVDAKLISDMTRLATEFFALPPEEKLKFDMSGGKKGGFIVSSHLQGEVVKDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP + RDYS WPDKPEGW   T EYS++LMG+ACKLLEVLSEAMGLE+EAL KACVD D
Sbjct: 135 SYPKQSRDYSRWPDKPEGWMEVTKEYSDQLMGVACKLLEVLSEAMGLEKEALTKACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ TKD G+TWITVQP++GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPIEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGHY+SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+ VYPLKIR+GEKP+LE
Sbjct: 255 VVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPLKIREGEKPVLE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQAQD 344
           +PI FSEMY  KM  D+E ARLKK   ++ QD
Sbjct: 315 EPIPFSEMYRRKMSKDLELARLKKLANEKHQD 346

[Close]
BLAST of Cucsa.322620 vs. TrEMBL
Match: D3JWB5_CITMA (Flavanone 3-hydroxylase OS=Citrus maxima GN=F3H PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 8.7e-149
Identity = 252/332 (75.90%), Postives = 291/332 (87.65%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L P FVR  D+RPKV YN+FS+EIP+ISL+GIDD G +RA I   IVEACE+WGIFQVVD
Sbjct: 15  LNPSFVRFQDERPKVAYNEFSNEIPVISLAGIDDVGGKRAEICKKIVEACEDWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD++++SDMTRLA EFF LP EEKL+FDMSGGK+GGF VSSHL+GE+VKDWRE+VT+F
Sbjct: 75  HGVDAKLISDMTRLATEFFALPPEEKLKFDMSGGKKGGFIVSSHLQGEVVKDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP + RDYS WPDKPEGW   T EYS++LMG+ACKLLEVLSEAMGLE+EAL KACVD D
Sbjct: 135 SYPKQSRDYSRWPDKPEGWMEVTKEYSDQLMGVACKLLEVLSEAMGLEKEALTKACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ TKD G+TWITVQP++GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPIEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGHY+SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+ VYPLKIR+GEKP+LE
Sbjct: 255 VVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPLKIREGEKPVLE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQAQD 344
           +PI FSEMY  KM  D+E ARLKK   ++ QD
Sbjct: 315 EPIPFSEMYRRKMSKDLELARLKKLANEKHQD 346

[Close]
BLAST of Cucsa.322620 vs. TAIR10
Match: AT3G51240.1 (AT3G51240.1 flavanone 3-hydroxylase)

HSP 1 Score: 512.3 bits (1318), Expect = 2.3e-145
Identity = 242/329 (73.56%), Postives = 282/329 (85.71%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L  +FVR  D+RPKV YN FSDEIP+ISL+GIDD   +R  I   IVEACE WGIFQVVD
Sbjct: 15  LNSKFVRDEDERPKVAYNVFSDEIPVISLAGIDDVDGKRGEICRQIVEACENWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD+ +V+DMTRLA +FF LP E+KLRFDMSGGK+GGF VSSHL+GE V+DWRE+VT+F
Sbjct: 75  HGVDTNLVADMTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP+  RDYS WPDKPEGW   T EYSE+LM LACKLLEVLSEAMGLE+E+L  ACVD D
Sbjct: 135 SYPVRNRDYSRWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ T+D G+TWITVQPV+GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGH++SNGRFK+ DHQAVVNS SSR+SIATFQ P+ ++ VYPLK+R+GEK ILE
Sbjct: 255 VVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQ 341
           +PITF+EMY  KM  D+E ARLKK  +++
Sbjct: 315 EPITFAEMYKRKMGRDLELARLKKLAKEE 343

[Close]
BLAST of Cucsa.322620 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 207.6 bits (527), Expect = 1.2e-53
Identity = 116/317 (36.59%), Postives = 183/317 (57.73%), Query Frame = 1 

Query: 10  TILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQV 69
           T L   +VR   DRP++      ++ P+I LS  D     R+ +   I +AC  +G FQV
Sbjct: 13  TTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTD-----RSFLIQQIHQACARFGFFQV 72

Query: 70  VDHGVDSEIVSDMTRLAGEFFGLPTEEKLR-FDMSGGKRGGFNVSSHLKGEIVKDWRELV 129
           ++HGV+ +I+ +M  +A EFF +  EEK++ +     K    + S ++K E V +WR+ +
Sbjct: 73  INHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYL 132

Query: 130 TFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACV 189
               YPI K   + WP  P  +    ++YS ++  +  K+ E++SE++GLE++ +KK   
Sbjct: 133 RLHCYPIHKY-VNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLG 192

Query: 190 DFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQD-QVGGLQVTKDGGRTWITVQPV 249
           +  Q + VN+YP CPEP+LT GL  HTDP  +T+LLQD  V GLQ+  DG   W  V P 
Sbjct: 193 EQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDG--QWFAVNPH 252

Query: 250 QGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLK----IR 309
             AFV+N+GD    +SNG +KS  H+AV N+++ R+S+A+F  P+  +++ P K      
Sbjct: 253 PDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAE 312

Query: 310 DGEKPILEKPITFSEMY 321
           D E   + K  T++E Y
Sbjct: 313 DDETKPVYKDFTYAEYY 321

[Close]
BLAST of Cucsa.322620 vs. TAIR10
Match: AT4G10490.1 (AT4G10490.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 206.8 bits (525), Expect = 2.1e-53
Identity = 120/311 (38.59%), Postives = 174/311 (55.95%), Query Frame = 1 

Query: 16  FVRHHDDRPKVPYNQFS-DEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVDHGV 75
           +VR   DRPK+   Q S D IP+I L   D +G  RA I +    AC   G FQ+ +HGV
Sbjct: 22  YVRPVSDRPKMSEVQTSGDSIPLIDLH--DLHGPNRADIINQFAHACSSCGFFQIKNHGV 81

Query: 76  DSEIVSDMTRLAGEFFGLPTEEKLR-FDMSGGKRGGFNVSSHLKGEIVKDWRELVTFFTY 135
             E +  M   A EFF     E+++ +     K    + S ++  E V +WR+ +    Y
Sbjct: 82  PEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCY 141

Query: 136 PIEKRDY-SFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFDQ 195
           PIE  D+ + WP  P  +   TAEY+  +  L   LLE +SE++GL ++ +        Q
Sbjct: 142 PIE--DFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQ 201

Query: 196 KIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAFV 255
            + +N+YP+CP+P+LT GL  H D   IT+LLQD+V GLQV KDG   WI V PV   F+
Sbjct: 202 HMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDG--KWIAVNPVPNTFI 261

Query: 256 VNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYP---LKIRDGEKPI 315
           VNLGD    +SN ++KS  H+AVVNS   RISI TF  PS+++++ P   L   + + P 
Sbjct: 262 VNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPA 321

Query: 316 LEKPITFSEMY 321
           + +  T++E +
Sbjct: 322 IYRNFTYAEYF 326

[Close]
BLAST of Cucsa.322620 vs. TAIR10
Match: AT4G10500.1 (AT4G10500.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 203.0 bits (515), Expect = 3.0e-52
Identity = 111/287 (38.68%), Postives = 166/287 (57.84%), Query Frame = 1 

Query: 16  FVRHHDDRPKVPYNQFS-DEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVDHGV 75
           +VR   DRP +   + S D IP+I L   D +G  RA+I   +  AC  +G FQ+ +HGV
Sbjct: 24  YVRPISDRPNLSEVESSGDSIPLIDLR--DLHGPNRAVIVQQLASACSTYGFFQIKNHGV 83

Query: 76  DSEIVSDMTRLAGEFFGLPTEEKLR-FDMSGGKRGGFNVSSHLKGEIVKDWRELVTFFTY 135
               V+ M  +A EFF  P  E+++ +     K    + S ++  + V +WR+ +    +
Sbjct: 84  PDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCF 143

Query: 136 PIEKRDY-SFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFDQ 195
           PIE  D+   WP  P  +   TAEY+  +  L  +LLE +SE++GLE + +        Q
Sbjct: 144 PIE--DFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQ 203

Query: 196 KIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAFV 255
            +  N+YP CPEP+LT GL  H DP  IT+LLQDQV GLQV KD    W+ V P+   F+
Sbjct: 204 HMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDD--KWVAVSPIPNTFI 263

Query: 256 VNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYP 300
           VN+GD    +SN ++KS  H+AVVN+++ R+SI TF +PS ++++ P
Sbjct: 264 VNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGP 304

[Close]
BLAST of Cucsa.322620 vs. TAIR10
Match: AT4G16330.2 (AT4G16330.2 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 192.2 bits (487), Expect = 5.3e-49
Identity = 103/271 (38.01%), Postives = 157/271 (57.93%), Query Frame = 1 

Query: 35  IPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVDHGVDSEIVSDMTRLAGEFF-GLP 94
           IP + LS  D         R +I +AC +WG F V++HGV   ++  M  L   FF   P
Sbjct: 67  IPTVDLSSSDS-------AREAIGDACRDWGAFHVINHGVPIHLLDRMRSLGLSFFQDSP 126

Query: 95  TEEKLRF--DMSGGKRGGFNVSSHL--KGEIVKDWRELVTFFTYPIEKRDYSFWPDKPEG 154
            EEKLR+  D +     G+     L  K ++V DWR+     T+P  +R+ S WP  P  
Sbjct: 127 MEEKLRYACDSTSAASEGYGSRMLLGAKDDVVLDWRDYFDHHTFPPSRRNPSHWPIHPSD 186

Query: 155 WAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFDQKIVVNFYPKCPEPDLTL 214
           +     EY +++  LA  LL ++SE++GL   ++++A  +  Q I V +YP CP+P+LTL
Sbjct: 187 YRQVVGEYGDEMKKLAQMLLGLISESLGLPCSSIEEAVGEIYQNITVTYYPPCPQPELTL 246

Query: 215 GLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAFVVNLGDHGHYVSNGRFKS 274
           GL+ H+D G ITLL+QD V GLQ+ KD    W+TV P+  A ++ + D    ++NGR+KS
Sbjct: 247 GLQSHSDFGAITLLIQDDVEGLQLYKDA--QWLTVPPISDAILILIADQTEIITNGRYKS 306

Query: 275 GDHQAVVNSKSSRISIATFQYPSQESIVYPL 301
             H+AV N+  +R+S+ATF  PS+ + + P+
Sbjct: 307 AQHRAVTNANRARLSVATFHDPSKTARIAPV 328

[Close]
BLAST of Cucsa.322620 vs. NCBI nr
Match: gi|449456807|ref|XP_004146140.1| (PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase [Cucumis sativus])

HSP 1 Score: 710.7 bits (1833), Expect = 1.3e-201
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 1 

Query: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60
           MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA
Sbjct: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60

Query: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120
           CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI
Sbjct: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120

Query: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180
           VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE
Sbjct: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180

Query: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240
           EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT
Sbjct: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240

Query: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300
           WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL
Sbjct: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300

Query: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 347
           KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA
Sbjct: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 346

[Close]
BLAST of Cucsa.322620 vs. NCBI nr
Match: gi|307136093|gb|ADN33941.1| (flavanone 3-hydroxylase [Cucumis melo subsp. melo])

HSP 1 Score: 653.7 bits (1685), Expect = 1.8e-184
Identity = 316/346 (91.33%), Postives = 331/346 (95.66%), Query Frame = 1 

Query: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60
           M  SLPHHPTILAPEF+RH DDRPKVPY+QFSDEIPIISL+GID +GERRA+IR SIVEA
Sbjct: 1   MPSSLPHHPTILAPEFLRHPDDRPKVPYDQFSDEIPIISLAGIDVDGERRAVIRRSIVEA 60

Query: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120
           CEEWGIFQVVDHGVDSEIVSDMTRLAG FF LPTEEKLRFDMSGGK+GGFN+SSHL+GE+
Sbjct: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGHFFALPTEEKLRFDMSGGKKGGFNISSHLQGEV 120

Query: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180
           VKDWRELVTFF+YPIEKRDYS WP+KPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLE+
Sbjct: 121 VKDWRELVTFFSYPIEKRDYSLWPNKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEK 180

Query: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240
            AL+K CVD DQKIVVNFYPKCPEPD TLGLKRHTDPGTITLLLQDQVGGLQ TKDGGRT
Sbjct: 181 GALRKTCVDLDQKIVVNFYPKCPEPDFTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRT 240

Query: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300
           WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSR+SIATFQYPSQESIVYPL
Sbjct: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRMSIATFQYPSQESIVYPL 300

Query: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 347
           KIRDGEKPILEKPITFSEMYHMKMKNDIE ARLKKQ Q+QAQDFVA
Sbjct: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIESARLKKQAQQQAQDFVA 346

[Close]
BLAST of Cucsa.322620 vs. NCBI nr
Match: gi|659096560|ref|XP_008449161.1| (PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase [Cucumis melo])

HSP 1 Score: 653.7 bits (1685), Expect = 1.8e-184
Identity = 316/346 (91.33%), Postives = 331/346 (95.66%), Query Frame = 1 

Query: 1   MTFSLPHHPTILAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEA 60
           M  SLPHHPTILAPEF+RH DDRPKVPY+QFSDEIPIISL+GID +GERRA+IR SIVEA
Sbjct: 1   MPSSLPHHPTILAPEFLRHPDDRPKVPYDQFSDEIPIISLAGIDVDGERRAVIRRSIVEA 60

Query: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEI 120
           CEEWGIFQVVDHGVDSEIVSDMTRLAG FF LPTEEKLRFDMSGGK+GGFN+SSHL+GE+
Sbjct: 61  CEEWGIFQVVDHGVDSEIVSDMTRLAGHFFALPTEEKLRFDMSGGKKGGFNISSHLQGEV 120

Query: 121 VKDWRELVTFFTYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEE 180
           VKDWRELVTFF+YPIEKRDYS WP+KPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLE+
Sbjct: 121 VKDWRELVTFFSYPIEKRDYSLWPNKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEK 180

Query: 181 EALKKACVDFDQKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRT 240
            AL+K CVD DQKIVVNFYPKCPEPD TLGLKRHTDPGTITLLLQDQVGGLQ TKDGGRT
Sbjct: 181 GALRKTCVDLDQKIVVNFYPKCPEPDFTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRT 240

Query: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPL 300
           WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSR+SIATFQYPSQESIVYPL
Sbjct: 241 WITVQPVQGAFVVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRMSIATFQYPSQESIVYPL 300

Query: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIEFARLKKQTQKQAQDFVA 347
           KIRDGEKPILEKPITFSEMYHMKMKNDIE ARLKKQ Q+QAQDFVA
Sbjct: 301 KIRDGEKPILEKPITFSEMYHMKMKNDIESARLKKQAQQQAQDFVA 346

[Close]
BLAST of Cucsa.322620 vs. NCBI nr
Match: gi|641852309|gb|KDO71174.1| (hypothetical protein CISIN_1g017946mg [Citrus sinensis])

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-148
Identity = 252/332 (75.90%), Postives = 291/332 (87.65%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L P FVR  D+RPKV YN+FS+EIP+ISL+GIDD G +RA I   IVEACE+WGIFQVVD
Sbjct: 15  LNPSFVRFQDERPKVAYNEFSNEIPVISLAGIDDVGGKRAEICKKIVEACEDWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD++++SDMTRLA EFF LP EEKL+FDMSGGK+GGF VSSHL+GE+VKDWRE+VT+F
Sbjct: 75  HGVDAKLISDMTRLATEFFALPPEEKLKFDMSGGKKGGFIVSSHLQGEVVKDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           ++P + RDYS WPDKPEGW   T EYS+KLMG+ACKLLEVLSEAMGLE+EAL KACVD D
Sbjct: 135 SFPKQSRDYSRWPDKPEGWMEVTKEYSDKLMGVACKLLEVLSEAMGLEKEALTKACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ TKD G+TWITVQP++GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPIEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGHY+SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+ VYPLKIR+GEKP+LE
Sbjct: 255 VVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPLKIREGEKPVLE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQAQD 344
           +PI FSEMY  KM  D+E ARLKK   ++ QD
Sbjct: 315 EPIPFSEMYRRKMSKDLELARLKKLANERHQD 346

[Close]
BLAST of Cucsa.322620 vs. NCBI nr
Match: gi|284518920|gb|ADB92595.1| (flavanone 3-hydroxylase [Citrus maxima])

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-148
Identity = 252/332 (75.90%), Postives = 291/332 (87.65%), Query Frame = 1 

Query: 12  LAPEFVRHHDDRPKVPYNQFSDEIPIISLSGIDDNGERRAMIRHSIVEACEEWGIFQVVD 71
           L P FVR  D+RPKV YN+FS+EIP+ISL+GIDD G +RA I   IVEACE+WGIFQVVD
Sbjct: 15  LNPSFVRFQDERPKVAYNEFSNEIPVISLAGIDDVGGKRAEICKKIVEACEDWGIFQVVD 74

Query: 72  HGVDSEIVSDMTRLAGEFFGLPTEEKLRFDMSGGKRGGFNVSSHLKGEIVKDWRELVTFF 131
           HGVD++++SDMTRLA EFF LP EEKL+FDMSGGK+GGF VSSHL+GE+VKDWRE+VT+F
Sbjct: 75  HGVDAKLISDMTRLATEFFALPPEEKLKFDMSGGKKGGFIVSSHLQGEVVKDWREIVTYF 134

Query: 132 TYPIEKRDYSFWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEEEALKKACVDFD 191
           +YP + RDYS WPDKPEGW   T EYS++LMG+ACKLLEVLSEAMGLE+EAL KACVD D
Sbjct: 135 SYPKQSRDYSRWPDKPEGWMEVTKEYSDQLMGVACKLLEVLSEAMGLEKEALTKACVDMD 194

Query: 192 QKIVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQVTKDGGRTWITVQPVQGAF 251
           QKIVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQ TKD G+TWITVQP++GAF
Sbjct: 195 QKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPIEGAF 254

Query: 252 VVNLGDHGHYVSNGRFKSGDHQAVVNSKSSRISIATFQYPSQESIVYPLKIRDGEKPILE 311
           VVNLGDHGHY+SNGRFK+ DHQAVVNS SSR+SIATFQ P+ E+ VYPLKIR+GEKP+LE
Sbjct: 255 VVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPLKIREGEKPVLE 314

Query: 312 KPITFSEMYHMKMKNDIEFARLKKQTQKQAQD 344
           +PI FSEMY  KM  D+E ARLKK   ++ QD
Sbjct: 315 EPIPFSEMYRRKMSKDLELARLKKLANEKHQD 346

The following BLAST results are available for this feature:
BLAST of Cucsa.322620 vs. Swiss-Prot
Match NameE-valueIdentityDescription
FL3H_PETHY1.1e-14775.38Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida GN=AN3 PE=... [more]
FL3H_MATIN7.5e-14674.47Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana GN=FHT PE... [more]
FL3H_MALDO2.4e-14473.00Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1[more]
FL3H_ARATH4.1e-14473.56Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana GN=F3H PE=1 SV=1[more]
FL3H_PETCR4.6e-14371.82Flavanone 3-dioxygenase OS=Petroselinum crispum GN=FHT PE=1 SV=1[more]
BLAST of Cucsa.322620 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L678_CUCSA8.8e-202100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002800 PE=3 SV=1[more]
E5RDD3_CUCME1.3e-18491.33Flavanone 3-hydroxylase OS=Cucumis melo subsp. melo PE=3 SV=1[more]
A0A067FUZ4_CITSI8.7e-14975.90Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017946mg PE=3 SV=1[more]
V4UHD8_9ROSI8.7e-14975.90Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025931mg PE=3 SV=1[more]
D3JWB5_CITMA8.7e-14975.90Flavanone 3-hydroxylase OS=Citrus maxima GN=F3H PE=2 SV=1[more]
BLAST of Cucsa.322620 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G51240.12.3e-14573.56 flavanone 3-hydroxylase[more]
AT5G24530.11.2e-5336.59 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G10490.12.1e-5338.59 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G10500.13.0e-5238.68 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G16330.25.3e-4938.01 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Cucsa.322620 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449456807|ref|XP_004146140.1|1.3e-201100.00PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase [Cucumis sativus][more]
gi|307136093|gb|ADN33941.1|1.8e-18491.33flavanone 3-hydroxylase [Cucumis melo subsp. melo][more]
gi|659096560|ref|XP_008449161.1|1.8e-18491.33PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase [Cucumis melo][more]
gi|641852309|gb|KDO71174.1|1.2e-14875.90hypothetical protein CISIN_1g017946mg [Citrus sinensis][more]
gi|284518920|gb|ADB92595.1|1.2e-14875.90flavanone 3-hydroxylase [Citrus maxima][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009813 flavonoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0045486 naringenin 3-dioxygenase activity
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.322620.1Cucsa.322620.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 192..288
score: 7.0
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 189..291
score: 1
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 35..145
score: 1.8
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 14..331
score: 3.5E
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 12..333
score: 1.3E
NoneNo IPR availablePANTHERPTHR10209:SF137NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASEcoord: 12..333
score: 1.3E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 13..326
score: 6.18