Cucsa.320550 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.320550
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Plant invertase/pectin methylesterase inhibitor
Location	scaffold03080 : 335140 .. 335718 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGCCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGTGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCGATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGA

mRNA sequence

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGCCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGTGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCGATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGA

Coding sequence (CDS)

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGCCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGTGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCGATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGA

Protein sequence

MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVSLLK*

Homology

[Close]

BLAST of Cucsa.320550 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.1e-27
Identity = 76/194 (39.18%), Postives = 112/194 (57.73%), Query Frame = 1


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MK S   L   A+L+IL        A++ P N  QFIKT+C  T YP +C  SLS  A T
Sbjct: 1   MKLSKSTLVFSALLVILAA------ASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           I ++   +   AL V+LT T+   + ++ L K    L  R    I DC+EE  DS++ + 
Sbjct: 61  IQNNPQELASTALQVSLTRTQQAQTFMKRLNKFKG-LKARQYAAIHDCLEEVEDSLDRVS 120

Query: 121 MAVEELKDNNKSRSET-----EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVV 180
            + +E+K+ + ++         ++ TWVSAALTD+ TCMDG  G  M+G +KES++  VV
Sbjct: 121 RSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVV 180

Query: 181 NVAQLTSIALSLVS 190
            VA++TS AL+LV+
Sbjct: 181 AVARVTSNALALVN 185

[Close]

BLAST of Cucsa.320550 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.2e-17
Identity = 51/158 (32.28%), Postives = 98/158 (62.03%), Query Frame = 1


Query: 37  IKTTCQSTPYPDLCLSSLS---DSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKS 96
           +K++C ST YPDLC S+++   +++  + S   ++ ++ L +  T        I+ L K 
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMS-LNITTTAVEHNYFGIQKLLKR 128

Query: 97  SNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD--NNKSRSE-TEDIRTWVSAALTDD 156
           +N LT R+   + DC+E   ++++EL  AVE+L++  N KS S+  +D++T +SAA+T+ 
Sbjct: 129 TN-LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQ 188

Query: 157 DTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLV 189
            TC+DG   D  N +V++++ +  V+V ++ S AL+++
Sbjct: 189 GTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMI 224

[Close]

BLAST of Cucsa.320550 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.2e-17
Identity = 51/158 (32.28%), Postives = 98/158 (62.03%), Query Frame = 1


Query: 37  IKTTCQSTPYPDLCLSSLS---DSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKS 96
           +K++C ST YPDLC S+++   +++  + S   ++ ++ L +  T        I+ L K 
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMS-LNITTTAVEHNYFGIQKLLKR 128

Query: 97  SNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD--NNKSRSE-TEDIRTWVSAALTDD 156
           +N LT R+   + DC+E   ++++EL  AVE+L++  N KS S+  +D++T +SAA+T+ 
Sbjct: 129 TN-LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQ 188

Query: 157 DTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLV 189
            TC+DG   D  N +V++++ +  V+V ++ S AL+++
Sbjct: 189 GTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMI 224

[Close]

BLAST of Cucsa.320550 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 6.3e-17
Identity = 54/191 (28.27%), Postives = 100/191 (52.36%), Query Frame = 1


Query: 12  AILLILLINQSNIIANSQPINDTQ-----------FIKTTCQSTPYPDLCLSSLSDSAAT 71
           AI L+LL +   I A +   N  Q            +K+ C ST YP+LC S+++ +   
Sbjct: 26  AIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGK 85

Query: 72  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 131
             +S   +  A+L +     +    A++ L      LTPR+   + DC+E   ++++EL 
Sbjct: 86  ELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDELH 145

Query: 132 MAVEELKDNNKSRS---ETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNV 189
           +AVE+L    K +S     +D++T +S+A+T+  TC+DG   D  +  V++++ +  V+V
Sbjct: 146 VAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHV 205

[Close]

BLAST of Cucsa.320550 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.1e-16
Identity = 49/158 (31.01%), Postives = 93/158 (58.86%), Query Frame = 1


Query: 37  IKTTCQSTPYPDLCLSSL---SDSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKS 96
           +K+ C +T +P+LC S++   SD +  + S   ++ ++ L + +   R    A++ L K+
Sbjct: 66  VKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELS-LNITVKAVRRNYYAVKELIKT 125

Query: 97  SNALTPRDSYVIRDCIEEFGDSVEELKMAVE--ELKDNNKSRSE-TEDIRTWVSAALTDD 156
              LTPR+   + DC+E   ++++EL  AVE  EL  N KS  E  ED++T +S+A+T+ 
Sbjct: 126 RKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQ 185

Query: 157 DTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLV 189
           +TC+DG   D  +  V++ + +   +V ++ S AL+++
Sbjct: 186 ETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMI 222

[Close]

BLAST of Cucsa.320550 vs. TrEMBL
Match: A0A0A0L1P3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 7.1e-100
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 1


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of Cucsa.320550 vs. TrEMBL
Match: A0A068UAV1_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00020316001 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.0e-34
Identity = 78/159 (49.06%), Postives = 111/159 (69.81%), Query Frame = 1


Query: 33  DTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAK 92
           +T+FI+T+C +T YP+LC +SLSD A  I S   L+  AAL+V+L   +STSS +  L++
Sbjct: 41  NTEFIRTSCSTTTYPELCYASLSDQATIIRSDPELLAHAALSVSLDTAKSTSSMMVKLSQ 100

Query: 93  SSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD--NNKSRSETEDIRTWVSAALTDD 152
           S + +TPR+   +RDC+EE GDSV+ELK ++ E+             DI+TWVSAALTD+
Sbjct: 101 S-HGMTPREVGAMRDCVEELGDSVDELKKSMGEMPQLRGPNFALTVNDIQTWVSAALTDE 160

Query: 153 DTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVS 190
           DTCMDG  G AMNGN K +++  +VNVA +TS AL+L++
Sbjct: 161 DTCMDGFAGKAMNGNTKIAVRNQIVNVAHMTSNALALIN 198

[Close]

BLAST of Cucsa.320550 vs. TrEMBL
Match: A5CBR6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00070 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 5.2e-34
Identity = 82/195 (42.05%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 1   MKSSYFPLPVKAILLIL-LINQSNIIANSQPIND---TQFIKTTCQSTPYPDLCLSSLSD 60
           M+ SYF   + A+ ++L L    N  + ++P  +    +FIKT+C++T YP LC SSL  
Sbjct: 1   MEGSYFFNALAALFILLQLTTHMNTCSAARPSPNEAYAEFIKTSCRTTTYPQLCTSSLLS 60

Query: 61  SAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSV 120
            A+ I +S  ++   AL++AL    STS+AI  L+K+ + L P ++  IRDC+E  GDS 
Sbjct: 61  YASKIQTSPKILADTALSIALATAHSTSTAITKLSKTQS-LKPGEAAAIRDCVEVLGDSE 120

Query: 121 EELKMAVEELK--DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMV 180
           +EL+M+++E++  +      +  DI+TWVSAALT+DDTCMD   G+AMNGNVK  ++  +
Sbjct: 121 DELQMSIQEMEHPEGKSFGLQMSDIQTWVSAALTNDDTCMDSFAGNAMNGNVKTIVRGYI 180

Query: 181 VNVAQLTSIALSLVS 190
           ++VAQ+TS+AL+L++
Sbjct: 181 LHVAQMTSVALALIN 194

[Close]

BLAST of Cucsa.320550 vs. TrEMBL
Match: A9PIS7_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.3e-33
Identity = 81/187 (43.32%), Postives = 126/187 (67.38%), Query Frame = 1


Query: 8   LPVKAILLILL---INQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSS 67
           L  K+++LI +   IN S+  A   P +  +FI+T+C +T YP LC +SLS  + TIH+S
Sbjct: 4   LTSKSLILIAISFFINSSSA-ATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTS 63

Query: 68  CHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVE 127
             L+  AAL V L+  +STS+ +  L++S + L P++   ++DC+EE  D+V EL+ +++
Sbjct: 64  PKLIANAALNVTLSSAKSTSTMMSKLSQS-HGLKPKEVSAMKDCVEELSDAVYELRESID 123

Query: 128 ELKDNNKSRSET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 187
           E+    +S  E    D+RTWVSAA+TD+ TC DG  G+AMNGN+K +I+  ++N+AQLTS
Sbjct: 124 EMGHAKQSNFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAIRGRIMNIAQLTS 183

Query: 188 IALSLVS 190
            AL+LV+
Sbjct: 184 NALALVN 188

[Close]

BLAST of Cucsa.320550 vs. TrEMBL
Match: B9ICT6_POPTR (Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14690g PE=4 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 5.7e-33
Identity = 80/187 (42.78%), Postives = 126/187 (67.38%), Query Frame = 1


Query: 8   LPVKAILLILL---INQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSS 67
           L  K+++LI +   IN S+  A   P +  +FI+T+C +T YP LC +SLS  + TIH+S
Sbjct: 4   LTSKSLILIAISFFINSSSA-ATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTS 63

Query: 68  CHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVE 127
             L+  AAL V L+  +STS+ +  L++S + L P++   ++DC+EE  D+V EL+ +++
Sbjct: 64  PKLIANAALNVTLSSAKSTSTMMSKLSQS-HGLKPKEVSAMKDCVEELSDAVYELRESID 123

Query: 128 ELKDNNKSRSET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 187
           E+    +S  E    D+RTWVSAA+TD+ TC DG  G+AMNGN+K +++  ++N+AQLTS
Sbjct: 124 EMDHVKRSDFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAVRGRIMNIAQLTS 183

Query: 188 IALSLVS 190
            AL+LV+
Sbjct: 184 NALALVN 188

[Close]

BLAST of Cucsa.320550 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 128.3 bits (321), Expect = 5.3e-30
Identity = 70/179 (39.11%), Postives = 111/179 (62.01%), Query Frame = 1


Query: 13  ILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAA 72
           I+ +L +  +     +    +T+F+K++C  T YP LC SSLS  A+ I +S  LM  AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 73  LTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNKS 132
           L + L   + TS+ +  L  S++ L P++   +RDC+EE GD++EEL+ ++ E+   + S
Sbjct: 80  LNITLASAKVTSAMMVRL--SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGS 139

Query: 133 RSET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVS 190
             E    DI+TWVSAALTD +TC DG  G+ M+G VK  ++  ++ +A LTS AL+L++
Sbjct: 140 NYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALIN 196

[Close]

BLAST of Cucsa.320550 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 118.2 bits (295), Expect = 5.5e-27
Identity = 65/187 (34.76%), Postives = 104/187 (55.61%), Query Frame = 1


Query: 13  ILLILLINQSNIIA----NSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 72
           IL +L ++ + I+       +   DT+FIK +C++T YPD C  SLS  A+ I      +
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 73  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 132
              AL V++   +S  + +  +      +T R    + DC+EE GD+V+ L  +++ELK 
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 133 NNKSRSETE------DIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 190
             +  S  +      ++RTW SAALTD+  CMDG  G AM G +K  I+  +V+VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of Cucsa.320550 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 115.9 bits (289), Expect = 2.7e-26
Identity = 71/184 (38.59%), Postives = 109/184 (59.24%), Query Frame = 1


Query: 13  ILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI-HSSCHLMTVA 72
           ILL + I QS+    ++  +D QFI ++CQ+T YP LC+ +LS  A  I H++   +   
Sbjct: 11  ILLSITILQSSSATPNRSESD-QFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQT 70

Query: 73  ALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNK 132
           AL ++L   +S +  +  L K +     R+   I+DCIE  G+SV+ L  +V+EL     
Sbjct: 71  ALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGH 130

Query: 133 SRSETE------DIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIAL 190
           + +  +      +++TWVSAALTD+ TC+DG    AM G VK  I+  VV+VAQ+TS AL
Sbjct: 131 AVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNAL 190

[Close]

BLAST of Cucsa.320550 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 114.0 bits (284), Expect = 1.0e-25
Identity = 70/189 (37.04%), Postives = 109/189 (57.67%), Query Frame = 1


Query: 7   PLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCH 66
           P P  + L  L  + S+   ++Q   +  +IKT+C  T Y  +C +SLS  A+TI S+  
Sbjct: 120 PPPSSSPLSSLSPSSSSSTYSNQ--TNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQ 179

Query: 67  LMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL 126
            + V AL + L+  +S S  +++++     LT  +   + DC+EE GDSV  L+ ++ EL
Sbjct: 180 KLAVIALNLTLSSAKSASKFVKNISHGGG-LTRLEVVAVADCVEEIGDSVTSLQDSIREL 239

Query: 127 -----KDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLT 186
                KD+ K      D+ TWVSAALT+DDTCMDG     +   VK+ ++  VV VA+LT
Sbjct: 240 DSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLT 299

Query: 187 SIALSLVSL 191
           S AL+L+++
Sbjct: 300 SNALALINM 303

[Close]

BLAST of Cucsa.320550 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 112.1 bits (279), Expect = 3.9e-25
Identity = 63/170 (37.06%), Postives = 99/170 (58.24%), Query Frame = 1


Query: 26  ANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSS 85
           A+ + IN   FI+++C++T YP LC+ SLS  A  I +S   +   A+ V L+  +ST  
Sbjct: 31  ASKKAIN---FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKL 90

Query: 86  AIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK------DNNKSRSETEDI 145
            +  L +    L  R+   I+DC+EE  D+V+ L  +V+ELK      D ++      + 
Sbjct: 91  FVSRLTRMKG-LKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNA 150

Query: 146 RTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVS 190
           +TW SAALTD++TC DG  G  M+G +K S++  ++NV   TS ALSL++
Sbjct: 151 QTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLIN 196

[Close]

BLAST of Cucsa.320550 vs. NCBI nr
Match: gi|449460487|ref|XP_004147977.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 1.0e-99
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 1


Query: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of Cucsa.320550 vs. NCBI nr
Match: gi|659096045|ref|XP_008448897.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 335.5 bits (859), Expect = 6.2e-89
Identity = 175/193 (90.67%), Postives = 184/193 (95.34%), Query Frame = 1


Query: 1   MKSSYFPLPVKAILLI-LLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60
           MKSSYFPLPVKAILL+ +LINQSN IANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSN-IANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120
            IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEEL
Sbjct: 61  AIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEEL 120

Query: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180
           KMA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQ
Sbjct: 121 KMAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQ 180

Query: 181 LTSIALSLVSLLK 193
           LTSIALSLVSLLK
Sbjct: 181 LTSIALSLVSLLK 192

[Close]

BLAST of Cucsa.320550 vs. NCBI nr
Match: gi|661891437|emb|CDP05319.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 153.3 bits (386), Expect = 4.3e-34
Identity = 78/159 (49.06%), Postives = 111/159 (69.81%), Query Frame = 1


Query: 33  DTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAK 92
           +T+FI+T+C +T YP+LC +SLSD A  I S   L+  AAL+V+L   +STSS +  L++
Sbjct: 41  NTEFIRTSCSTTTYPELCYASLSDQATIIRSDPELLAHAALSVSLDTAKSTSSMMVKLSQ 100

Query: 93  SSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD--NNKSRSETEDIRTWVSAALTDD 152
           S + +TPR+   +RDC+EE GDSV+ELK ++ E+             DI+TWVSAALTD+
Sbjct: 101 S-HGMTPREVGAMRDCVEELGDSVDELKKSMGEMPQLRGPNFALTVNDIQTWVSAALTDE 160

Query: 153 DTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVS 190
           DTCMDG  G AMNGN K +++  +VNVA +TS AL+L++
Sbjct: 161 DTCMDGFAGKAMNGNTKIAVRNQIVNVAHMTSNALALIN 198

[Close]

BLAST of Cucsa.320550 vs. NCBI nr
Match: gi|225426925|ref|XP_002264891.1| (PREDICTED: 21 kDa protein [Vitis vinifera])

HSP 1 Score: 152.5 bits (384), Expect = 7.4e-34
Identity = 82/195 (42.05%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 1   MKSSYFPLPVKAILLIL-LINQSNIIANSQPIND---TQFIKTTCQSTPYPDLCLSSLSD 60
           M+ SYF   + A+ ++L L    N  + ++P  +    +FIKT+C++T YP LC SSL  
Sbjct: 1   MEGSYFFNALAALFILLQLTTHMNTCSAARPSPNEAYAEFIKTSCRTTTYPQLCTSSLLS 60

Query: 61  SAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSV 120
            A+ I +S  ++   AL++AL    STS+AI  L+K+ + L P ++  IRDC+E  GDS 
Sbjct: 61  YASKIQTSPKILADTALSIALATAHSTSTAITKLSKTQS-LKPGEAAAIRDCVEVLGDSE 120

Query: 121 EELKMAVEELK--DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMV 180
           +EL+M+++E++  +      +  DI+TWVSAALT+DDTCMD   G+AMNGNVK  ++  +
Sbjct: 121 DELQMSIQEMEHPEGKSFGLQMSDIQTWVSAALTNDDTCMDSFAGNAMNGNVKTIVRGYI 180

Query: 181 VNVAQLTSIALSLVS 190
           ++VAQ+TS+AL+L++
Sbjct: 181 LHVAQMTSVALALIN 194

[Close]

BLAST of Cucsa.320550 vs. NCBI nr
Match: gi|118488943|gb|ABK96280.1| (unknown [Populus trichocarpa x Populus deltoides])

HSP 1 Score: 149.8 bits (377), Expect = 4.8e-33
Identity = 81/187 (43.32%), Postives = 126/187 (67.38%), Query Frame = 1


Query: 8   LPVKAILLILL---INQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSS 67
           L  K+++LI +   IN S+  A   P +  +FI+T+C +T YP LC +SLS  + TIH+S
Sbjct: 4   LTSKSLILIAISFFINSSSA-ATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTS 63

Query: 68  CHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVE 127
             L+  AAL V L+  +STS+ +  L++S + L P++   ++DC+EE  D+V EL+ +++
Sbjct: 64  PKLIANAALNVTLSSAKSTSTMMSKLSQS-HGLKPKEVSAMKDCVEELSDAVYELRESID 123

Query: 128 ELKDNNKSRSET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 187
           E+    +S  E    D+RTWVSAA+TD+ TC DG  G+AMNGN+K +I+  ++N+AQLTS
Sbjct: 124 EMGHAKQSNFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAIRGRIMNIAQLTS 183

Query: 188 IALSLVS 190
            AL+LV+
Sbjct: 184 NALALVN 188

The following BLAST results are available for this feature:

BLAST of Cucsa.320550 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	5.1e-27	39.18	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME3_CITSI	2.2e-17	32.28	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]
PME1_CITSI	2.2e-17	32.28	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]
PME2_ARATH	6.3e-17	28.27	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PMEU1_SOLLC	3.1e-16	31.01	Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2...	[more]

BLAST of Cucsa.320550 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1P3_CUCSA	7.1e-100	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G035340 PE=4 SV=1	[more]
A0A068UAV1_COFCA	3.0e-34	49.06	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00020316001 PE=4 SV=1	[more]
A5CBR6_VITVI	5.2e-34	42.05	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00070 PE=4 SV=...	[more]
A9PIS7_9ROSI	3.3e-33	43.32	Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2...	[more]
B9ICT6_POPTR	5.7e-33	42.78	Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14690g PE=4...	[more]

BLAST of Cucsa.320550 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62360.1	5.3e-30	39.11	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	5.5e-27	34.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	2.7e-26	38.59	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	1.0e-25	37.04	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	3.9e-25	37.06	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Cucsa.320550 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449460487\|ref\|XP_004147977.1\|	1.0e-99	100.00	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|659096045\|ref\|XP_008448897.1\|	6.2e-89	90.67	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
gi\|661891437\|emb\|CDP05319.1\|	4.3e-34	49.06	unnamed protein product [Coffea canephora]	[more]
gi\|225426925\|ref\|XP_002264891.1\|	7.4e-34	42.05	PREDICTED: 21 kDa protein [Vitis vinifera]	[more]
gi\|118488943\|gb\|ABK96280.1\|	4.8e-33	43.32	unknown [Populus trichocarpa x Populus deltoides]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.320550.1	Cucsa.320550.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 34..191 score: 1.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..187 score: 2.0
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..187 score: 1.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 13..189 score: 5.4
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 35..191 score: 8.37
None	No IPR available	unknown	Coil	Coil	coord: 109..136 scor
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..188 score: 5.3
None	No IPR available	PANTHER	PTHR31080:SF19	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 1..188 score: 5.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.320550	Cla005681	Watermelon (97103) v1	cgywmB585
Cucsa.320550	Csa3G035340	Cucumber (Chinese Long) v2	cgycuB458
Cucsa.320550	MELO3C013975	Melon (DHL92) v3.5.1	cgymeB532
Cucsa.320550	ClCG10G003050	Watermelon (Charleston Gray)	cgywcgB556
Cucsa.320550	CSPI03G03060	Wild cucumber (PI 183967)	cgycpiB484
Cucsa.320550	CmaCh15G011540	Cucurbita maxima (Rimu)	cgycmaB0886
Cucsa.320550	CmaCh16G010510	Cucurbita maxima (Rimu)	cgycmaB0888
Cucsa.320550	CmoCh16G010990	Cucurbita moschata (Rifu)	cgycmoB0886
Cucsa.320550	Lsi07G013360	Bottle gourd (USVL1VR-Ls)	cgylsiB530
Cucsa.320550	Lsi09G003880	Bottle gourd (USVL1VR-Ls)	cgylsiB526
Cucsa.320550	Cp4.1LG14g08760	Cucurbita pepo (Zucchini)	cgycpeB0846
Cucsa.320550	MELO3C005567.2	Melon (DHL92) v3.6.1	cgymedB531
Cucsa.320550	MELO3C013975.2	Melon (DHL92) v3.6.1	cgymedB533
Cucsa.320550	CsaV3_5G003880	Cucumber (Chinese Long) v3	cgycucB502
Cucsa.320550	CsaV3_3G003090	Cucumber (Chinese Long) v3	cgycucB499
Cucsa.320550	Cla97C01G003880	Watermelon (97103) v2	cgywmbB560
Cucsa.320550	Cla97C10G187580	Watermelon (97103) v2	cgywmbB562
Cucsa.320550	Bhi12G000373	Wax gourd	cgywgoB649
Cucsa.320550	BhiUN285G21	Wax gourd	cgywgoB650
Cucsa.320550	Carg25267	Silver-seed gourd	carcgyB0984

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cucsa.320550	Cucsa.302150	Cucumber (Gy14) v1	cgycgyB141

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.320550	Silver-seed gourd	carcgyB0990
Cucsa.320550	Cucurbita maxima (Rimu)	cgycmaB0897
Cucsa.320550	Cucurbita moschata (Rifu)	cgycmoB0884
Cucsa.320550	Cucurbita moschata (Rifu)	cgycmoB0893
Cucsa.320550	Wild cucumber (PI 183967)	cgycpiB487
Cucsa.320550	Cucumber (Chinese Long) v2	cgycuB461
Cucsa.320550	Melon (DHL92) v3.5.1	cgymeB530
Cucsa.320550	Watermelon (Charleston Gray)	cgywcgB557
Cucsa.320550	Watermelon (97103) v1	cgywmB588
Cucsa.320550	Cucurbita pepo (Zucchini)	cgycpeB0844
Cucsa.320550	Cucurbita pepo (Zucchini)	cgycpeB0857