Cucsa.238230 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.238230 vs. Swiss-Prot
Match: ENG2_SCHPO (Putative endo-1,3(4)-beta-glucanase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=eng2 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 2.1e-36
Identity = 166/672 (24.70%), Postives = 287/672 (42.71%), Query Frame = 1

Query: 67  PSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSY 126
           P   S   P P +  +  L   P+ +N F+ N  +     P + HPY +  +  + S  Y
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSV--TWLNGSSYY 73

Query: 127 PSILSNSASECQIFTPDLTISPSEK-INPLPQKSHVISSFNDLSVTLDIPSSNLRFYL-- 186
              +S+     ++F PD    P +   NP    S +IS+    S  L     +  F +  
Sbjct: 74  GLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQA 133

Query: 187 -----VRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALT-------------KYTIKLK 246
                  GS  +   +  G+ F +S  +  ++  FN+++              KY I+L 
Sbjct: 134 TLSATTSGSGTIILPIVAGMGF-VSGYYTNLTPVFNSSILFSSITKINFSRGYKYRIQLT 193

Query: 247 NNQTWLIY-------SSFPINLTHNLSMITSGGFAGIIRIAALPNSDLEC---EQILDRF 306
           + + W +Y       S+F + L  N  + TS  F G+I+I  +PN  +     + I D  
Sbjct: 194 DGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDAS 253

Query: 307 SSCYP--VLGEAQITKPFC-LEYKWETKGWGDL--LMLAHPLHLRLLGGSDDNVVILDKF 366
           +  Y   +   AQ++       +++ T G+ +L  LM A P H++  G SD         
Sbjct: 254 AGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSFG-SDTQAYKTGLG 313

Query: 367 KYKSIDGELVGVVGSSWAL--KPEPISVSWHSI------RGVEEESFAEIISALRKDV-- 426
              +  G +      +W L  K  P  V +  I            + A I +A   D+  
Sbjct: 314 LASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPWNGGSNTYSPTALAAIRAACATDINF 373

Query: 427 EALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEMVPEIRKFLIGAIEPWLNGTF 486
           + +N++++     S Y+ GK++A  A++ ++A  +     +       +           
Sbjct: 374 DVVNASNL----DSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAMARFTTNT 433

Query: 487 EGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLGYFLYAIAVLVKIDPAW-GRK 546
           +    VYD  + GI+S  G     AD+G   YN+HH H GY +YA AV+  +DP+W    
Sbjct: 434 QMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPSWLVND 493

Query: 547 YSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGLSEFTDGRSQESVSEAVNAY 606
               V +L+ D  N S  +++ F   R FD +  HSW TG+ E  DG+ +ES SE  N  
Sbjct: 494 NIRYVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEESTSEDFNFL 553

Query: 607 YSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGETTLYKEEFVKENRVVGVLWS 666
           Y+  L G+   D  L++  +++  L +    +   I    TT  +   +  N V G+ + 
Sbjct: 554 YATKLWGMVRNDTVLINRANLM--LAVLKNSLNTYIYMTPTTSVQPSQILGNYVTGITFM 613

Query: 667 NKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPSLGREGVGEGW 692
           NK D   +F+  E+  C+ GI ++P  PI+  L S   +V++  N  +  +        W
Sbjct: 614 NKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSP-SYVQQDWNAKIAPI-INTFSNNW 668

BLAST of Cucsa.238230 vs. Swiss-Prot
Match: ENG1_ARTBC (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 3.6e-36
Identity = 195/754 (25.86%), Postives = 314/754 (41.64%), Query Frame = 1

Query: 27  PPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLPDPSVFFSPELL 86
           P P    P S  PS P   PP +   +   +P    P   P+   +    P      E  
Sbjct: 166 PGPTATSPTSGIPSQPSA-PPATMAGQDIFQPIAKDPI--PANIKSRDDHPVKANHIENP 225

Query: 87  SAPLPSNSFFQNFTLNNGDQPEYIHPYLI-------KSSLSSISVSYPSILSNSASECQI 146
           + P+ +N F+ NF L N     + HPY +        +S   +++S+      +  E   
Sbjct: 226 TGPISTNKFYANFFLGNQTSTTFTHPYTMIWAKGDKNASSFGMAISHVEPSQRATGEPNN 285

Query: 147 FTPDLTISPSEKINPLPQKSHVISSFN-DLSVTLDI--PSSNLRFYLVR----GSPFLTF 206
             P   +     INP+  KS V+S+     S T+ +  P +     ++R     S  +TF
Sbjct: 286 KLPGNPVR--YYINPVGIKSLVLSASELKESTTMSVAKPQAFSAQAILRPTGGSSESITF 345

Query: 207 TVSKGVAFSISTIHEVISFSFNNALT---------------KYTIKLKNNQTWLIY---- 266
            + +G+ F I+ I+  +  +  +A+                KY I L++++ WL+Y    
Sbjct: 346 PLVQGMGF-ITAIYNNLQPAIQSAVLFRKVEPAGSPQGGIFKYKITLEDDKNWLLYVIPE 405

Query: 267 --SSFPINLTHNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYP----VLGEAQI 326
             +   + L  N  +    GF G+I++A  P+++ E E I D+ +  Y     + G    
Sbjct: 406 NGADPKLKLEGNKLISGPTGFKGVIQVAKNPSAE-EGEGIYDKSAGSYATDIKISGSVGT 465

Query: 327 TKPFCLEYKWETKGWG-DLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSS 386
                 ++ +E  G G  L+M A P H+     +  N     K    +  G     VG S
Sbjct: 466 DGTGTYKFSFEKAGKGAPLVMYALPHHVESFDDATKNTKKNMKLS-TTTKGMATACVGDS 525

Query: 387 WALKPEPISVS-----WHS--------IRGVEEESFAEIISALRKDVEA-LNSTSMILTT 446
           W +    + +S     W            G +    A   + L +D+E   N  SM  + 
Sbjct: 526 WTMVEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNELSQDMELQTNLNSMYFSG 585

Query: 447 KSPYSYGKLIARAARL----AVIAEEVRSLEMVPEIRKFLIGAIEPWLNGTFEGNGFVYD 506
           K    +   I     L    A  +  + SL+     ++F+    +  L         VYD
Sbjct: 586 KGLNKFAGAIYTVQELVGDKAAASGPLNSLK--ESFKRFVDNKQQIPL---------VYD 645

Query: 507 EKWGGIVSKEGAF---DYSADFGFGMYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQVY 566
             W G+VS  G +   D   DFG  +YN+HH H GYF+   A+L K+DPAW       V 
Sbjct: 646 NVWKGVVSS-GTYEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVN 705

Query: 567 SLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGLSEFTDGRSQESVSEAVNAYYSAALV 626
            L+ D  N S   +  FP  R FD Y  HSW  GL E +DG+ QES SE     Y+  + 
Sbjct: 706 MLVRDSGN-SVDNDEHFPFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMW 765

Query: 627 GLAYGDAHLVSIGSILAALEIKAGQMWWQIREGETTLYKEEFVKENRVVGVLWSNKRDSG 686
           G   GD  + + G+++  +  +    ++ + + +     + F+  N+V G+L+ NK D  
Sbjct: 766 GKTSGDKSMEARGNLMLGILARTLNNYF-LMKNDNVNQPKNFI-GNKVTGILFENKIDHT 825

Query: 687 LWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNW-ALPSLG----REGVGEGWK 711
            +F  +   E   GI +LP+LP +    S   FV+E   W A+ + G     E V  GWK
Sbjct: 826 TYFGAN--LEYIQGIHMLPLLPNSAYTRS-AEFVKE--EWEAMFASGAAAPAEKVTGGWK 885

BLAST of Cucsa.238230 vs. Swiss-Prot
Match: ENG1_YEAST (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DSE4 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 7.7e-31
Identity = 162/688 (23.55%), Postives = 276/688 (40.12%), Query Frame = 1

Query: 67   PSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSI---- 126
            P T     P+P           P+ +N F+ N  + + + P +++PY +    SS     
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSSSYGFA 470

Query: 127  -------SVSYPSILSNSASECQI--------------FTPDLTISPSEKINPLPQKSHV 186
                     SY    S+  +E  +              F   +T+   E    L     V
Sbjct: 471  VQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMT--LSSTRVV 530

Query: 187  ISSFNDLSVTLDIPSSNLRFYLVRGSPFLT------FTVSKGVAFSISTIHEVISFSFNN 246
            +S  ND S  L+IP       LV+G  F T           G +   +TI    S +   
Sbjct: 531  LSESNDSSNYLEIP-------LVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQ 590

Query: 247  ALTKYTIKLKNNQTWLIY---------SSFPINLTHNLSMITSGGFAGIIRIAALPNSDL 306
             + KY I L N  TWL Y         + F + ++    +  S    G+I   A+  S+ 
Sbjct: 591  GILKYRITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPSET 650

Query: 307  ECEQILDRFSSCYPVLGEAQITKP---FCLEYKWETKGW---GDLLMLAHPLHLRLLGGS 366
            + E   D+ +  Y    + Q          E+ + T+G    G  ++ A P H      S
Sbjct: 651  DYEVFYDQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHH-----ES 710

Query: 367  DDNVVILDKFKYKSIDGELVGVVGS--------SWALKPEPISVSWHSIRGVEEESFAEI 426
              + ++ D +    +     GV+          S +L  +   + W S  G     +++ 
Sbjct: 711  SFSDIMQDYYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKE 770

Query: 427  ISALRKDV---EALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEMVPEIRKFLI 486
               L  +V   E   S S  ++  + Y  GK+I + + + +   E+   E   +     I
Sbjct: 771  QLQLLAEVANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENI 830

Query: 487  GAIEPWLNGTFEGNGFVYDEKWGGIVSKE--GAFDYSADFGFGMYNNHHHHLGYFLYAIA 546
             +    L    +    +YD K+ G+VS    G+     DFG   YN+HH H GY ++A A
Sbjct: 831  KSAFDILLQNEQTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAA 890

Query: 547  VL----VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGLSEF 606
            V+     K++  W       V SL+ D+ N S + +  F + R FD +  HSW  GL E 
Sbjct: 891  VIGYVDSKLNGTWAADNKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAGLYEN 950

Query: 607  TDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGETTLY 666
             +G+++ES SE  N  Y+  L G   GD  +   G ++ ++ +K     +   + + T+ 
Sbjct: 951  GNGKNEESSSEDYNFAYAMKLWGATIGDQSMELRGDLMISI-MKDAMNDYFYYQNDNTVE 1010

Query: 667  KEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELV 692
             EE +  N+V G+L+ N  D   +F  +   E   GI +LPI P++  + S+     E  
Sbjct: 1011 PEEII-GNKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQ 1070

BLAST of Cucsa.238230 vs. Swiss-Prot
Match: SSGP_VOLCA (Sulfated surface glycoprotein 185 OS=Volvox carteri PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.2e-13
Identity = 36/60 (60.00%), Postives = 36/60 (60.00%), Query Frame = 1

Query: 19  SPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLPDPS 78
           SPP   PPPPPPPPPPP PPPSPPPP PP   PP P   PS   P   PS    V P PS
Sbjct: 259 SPP---PPPPPPPPPPPPPPPSPPPPPPPPPPPPPPPPPPSPSPPRKPPSPSPPVPPPPS 315

HSP 2 Score: 71.6 bits (174), Expect = 4.0e-11
Identity = 35/64 (54.69%), Postives = 35/64 (54.69%), Query Frame = 1

Query: 19  SPPWCLPPPPPP----PPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVL 78
           SPP   P PPPP    PPPPP PPPSPPPP PP   PP P   PS   P   PS    V 
Sbjct: 252 SPPPPSPSPPPPPPPPPPPPPPPPPSPPPPPPPPPPPPPPPPPPSPSPPRKPPSPSPPVP 311

HSP 3 Score: 70.9 bits (172), Expect = 6.8e-11
Identity = 32/62 (51.61%), Postives = 38/62 (61.29%), Query Frame = 1

Query: 20  PPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFL-FPSTQSTVLPDPS 79
           PP   PPPPPPP PPP PPP PPPP PP    P P +KP  P+P +  P +  +VLP  +
Sbjct: 265 PPPPPPPPPPPPSPPPPPPPPPPPPPPPPPPSPSPPRKPPSPSPPVPPPPSPPSVLPAAT 324

Query: 80  VF 81
            F
Sbjct: 325 GF 326

HSP 4 Score: 68.2 bits (165), Expect = 4.4e-10
Identity = 35/73 (47.95%), Postives = 35/73 (47.95%), Query Frame = 1

Query: 19  SPPWCLPPP-------------PPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFL 78
           SPP    PP             PPPPPPPP PPPSPPPP PP   PP P   PS   P  
Sbjct: 243 SPPPSPRPPSPPPPSPSPPPPPPPPPPPPPPPPPSPPPPPPPPPPPPPPPPPPSPSPPRK 302

HSP 5 Score: 66.6 bits (161), Expect = 1.3e-09
Identity = 32/67 (47.76%), Postives = 33/67 (49.25%), Query Frame = 1

Query: 25  PPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLPDPSVFFSPE 84
           PPPP P PPPP PPP PPPP PP S PP P   P  P P           P PS    P 
Sbjct: 253 PPPPSPSPPPPPPPPPPPPPPPPPSPPPPPPPPPPPPPP--------PPPPSPSPPRKPP 311

Query: 85  LLSAPLP 92
             S P+P
Sbjct: 313 SPSPPVP 311

HSP 6 Score: 64.3 bits (155), Expect = 6.3e-09
Identity = 35/76 (46.05%), Postives = 36/76 (47.37%), Query Frame = 1

Query: 19  SPPWCLPPPPPPPP---PPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLP 78
           SPP    PP PPPP   PPP PPP PPPP PP   PP P   P  P P   P       P
Sbjct: 243 SPPPSPRPPSPPPPSPSPPPPPPPPPPPPPPPPPSPPPPPPPPPPPPPPPPP-------P 302

Query: 79  DPSVFFSPELLSAPLP 92
            PS    P   S P+P
Sbjct: 303 SPSPPRKPPSPSPPVP 311

HSP 7 Score: 53.5 bits (127), Expect = 1.1e-05
Identity = 32/82 (39.02%), Postives = 33/82 (40.24%), Query Frame = 1

Query: 26  PP------------PPPPPPPPSPPP----SPPPPQPPESQPPEPSQKPSEPTPFLFPST 85
           PP            PPP P PPSPPP     PPPP PP   PP P   P  P P   P  
Sbjct: 230 PPSPQPTASSRPPSPPPSPRPPSPPPPSPSPPPPPPPPPPPPPPPPPSPPPPPPPPPPPP 289

Query: 86  QSTVLPDPSVFFSPELLSAPLP 92
                P PS    P   S P+P
Sbjct: 290 PPPPPPSPSPPRKPPSPSPPVP 311

BLAST of Cucsa.238230 vs. Swiss-Prot
Match: LRX6_ARATH (Leucine-rich repeat extensin-like protein 6 OS=Arabidopsis thaliana GN=LRX6 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.1e-12
Identity = 39/95 (41.05%), Postives = 47/95 (49.47%), Query Frame = 1

Query: 19  SPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVL--PD 78
           SPP   PPPPPPPPPPP PPP PPPP PP    P P   P  P P+++P      +  P 
Sbjct: 378 SPPPPPPPPPPPPPPPPPPPPPPPPPPPPPYVYPSPPPPPPSPPPYVYPPPPPPYVYPPP 437

Query: 79  PSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIH 112
           PS    P +   P PS   +   +    D P  +H
Sbjct: 438 PS---PPYVYPPPPPSPQPYMYPSPPCNDLPTPVH 469

BLAST of Cucsa.238230 vs. TrEMBL
Match: A0A0A0KUJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643380 PE=4 SV=1)

HSP 1 Score: 1503.8 bits (3892), Expect = 0.0e+00
Identity = 736/736 (100.00%), Postives = 736/736 (100.00%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE
Sbjct: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR
Sbjct: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300
           THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420
           IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480
           VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540

Query: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600
           SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET
Sbjct: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
           TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEEQNINNSKIHLV 737
           RGKEEQNINNSKIHLV
Sbjct: 721 RGKEEQNINNSKIHLV 736

BLAST of Cucsa.238230 vs. TrEMBL
Match: E5GCT6_CUCME (Beta-glucan-binding protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 1425.6 bits (3689), Expect = 0.0e+00
Identity = 703/736 (95.52%), Postives = 714/736 (97.01%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           M KKIER L R  KGKRHSPP  LPPPPPPPPPPP         Q PESQPPEPSQKPSE
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPP---------QTPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           SISVSYPSILS  ASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR
Sbjct: 121 SISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300
           THNLSMITSGGFAGIIRIAALPNSDLECE+ILDRFSSCYPVLGEAQ TKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHLRLL GSDDNV+ILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420
           IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLE+
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEV 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480
           VPEIRKFLIGAIEPWLNGTFEGNGF+YDEKWGGIV+KEGAFD+SADFGFG+YNNHHHHLG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGL 540

Query: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600
           +EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGS+LAALEIKAGQMWWQIREGET
Sbjct: 541 AEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGET 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
           TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEEQNINNSKIHLV 737
           RGKEE+NI+NSKIHLV
Sbjct: 721 RGKEEENISNSKIHLV 727

BLAST of Cucsa.238230 vs. TrEMBL
Match: W9R5F5_9ROSA (Endo-1,3(4)-beta-glucanase 1 OS=Morus notabilis GN=L484_009128 PE=4 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 1.5e-299
Identity = 503/739 (68.06%), Postives = 595/739 (80.51%), Query Frame = 1

Query: 1   MLKKIER----LLGRVIKGKRHSPPWCLPPPPPPPP-PPPSPPPSPPPPQPPESQPPEPS 60
           MLKK+ R    L+ +  K  R  P +  P P  P   P PS P     P+PP+       
Sbjct: 1   MLKKLRRRVKTLVTKTFKKPRRRPEFSTPSPSTPVEVPSPSTPEEVVEPEPPKMSLSSSF 60

Query: 61  QKPSEPTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLI 120
           QK S   PFLFP  QSTV+ DPS+FFSP+LLS PLP+NSFFQNF L NGDQPEYIHPYL+
Sbjct: 61  QKRSN-APFLFPKAQSTVVSDPSLFFSPDLLSTPLPTNSFFQNFVLKNGDQPEYIHPYLV 120

Query: 121 KSSLSSISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIP 180
           KSS SS+SV YPS LSNSA   QIF  DLT+S +EK  P  QK H+ISSFNDLS+TLDIP
Sbjct: 121 KSSNSSLSVCYPSRLSNSAFLYQIFNADLTVSAAEKTAPDSQKPHLISSFNDLSLTLDIP 180

Query: 181 SSNLRFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSS 240
           SSNLRF+LVRGSPFLT T S     SISTIH ++SFS NN+ TKYT+KL NNQTWLIY+S
Sbjct: 181 SSNLRFFLVRGSPFLTCTASAKTEISISTIHAILSFSSNNSQTKYTVKLNNNQTWLIYTS 240

Query: 241 FPINLTHNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEY 300
            PI+ +H+LS++TS GF+GI+RIA LP+S+ E E ILDRFSSCYPV GE   TKPFCLEY
Sbjct: 241 SPISFSHSLSLLTSNGFSGILRIAILPDSNPEFEAILDRFSSCYPVSGEVSFTKPFCLEY 300

Query: 301 KWETKGWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPIS 360
           KWE KGWGDLLMLAHPLHL+LL G D  V +L++FKYKSIDG+LVGVVG SW LKP+P+S
Sbjct: 301 KWEKKGWGDLLMLAHPLHLKLLKGDDRGVTVLEEFKYKSIDGDLVGVVGDSWTLKPDPVS 360

Query: 361 VSWHSIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEV 420
           V+WHSI GV+EE++ EI++AL+KDVEAL++ +  +TT + Y YGKLIARAAR A+IAEEV
Sbjct: 361 VTWHSIEGVKEEAYEEIVAALQKDVEALDAAA--ITTNASYFYGKLIARAARFALIAEEV 420

Query: 421 RSLEMVPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNH 480
           R  +++P+IRKFL  AIEPWL+GTF GNGF+Y+EKWGGI++K+G  D  ADFGFG+YN+H
Sbjct: 421 RFFDVIPKIRKFLKEAIEPWLDGTFNGNGFLYEEKWGGIITKQGQGDGGADFGFGIYNDH 480

Query: 481 HHHLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHS 540
           H HLG+FLYAIAVL KIDPAWGRKY PQ YS+MAD MNLSRRA S +P+LRCFD YKLHS
Sbjct: 481 HFHLGHFLYAIAVLAKIDPAWGRKYKPQAYSMMADFMNLSRRAKSNYPRLRCFDLYKLHS 540

Query: 541 WGTGLSEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQI 600
           W  GL+EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+IGS+L ALEI+A QMWW +
Sbjct: 541 WAGGLTEFADGRNQESSSEAVNAYYSAALMGLAYGDTHLVAIGSLLTALEIQAAQMWWHV 600

Query: 601 REGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSD 660
           +EG+  LY E+F +ENRVVGVLWSNKRDSGLWFAP+ W+ECRLGIQVLP+LPITE+L SD
Sbjct: 601 KEGD-KLYDEDFTRENRVVGVLWSNKRDSGLWFAPAAWRECRLGIQVLPLLPITEVLFSD 660

Query: 661 VGFVRELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLL 720
           VGF RELVNW LP+L REGVGEGWKGFV+ALE IYDK+ +LQKI++L  FDDGNSLTNLL
Sbjct: 661 VGFTRELVNWTLPALQREGVGEGWKGFVYALEGIYDKETALQKIKSLNGFDDGNSLTNLL 720

Query: 721 WWVHSRGKEEQNINNSKIH 735
           WW+HSR +E++       H
Sbjct: 721 WWIHSRDQEKEEFGGGGKH 735

BLAST of Cucsa.238230 vs. TrEMBL
Match: B9H3S1_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s10500g PE=4 SV=2)

HSP 1 Score: 1029.2 bits (2660), Expect = 2.4e-297
Identity = 497/727 (68.36%), Postives = 590/727 (81.16%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPP-SPPPPQPPESQPPEPSQKPS 60
           MLKK++R +   I+     P   +P  PP P P PSPPP SP PPQ P    P+ S    
Sbjct: 1   MLKKLKRRVLTSIRKSFRKPRKRMPYKPPTPSPSPSPPPQSPSPPQTPTM--PQHSHHRP 60

Query: 61  EPTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSL 120
              PFLFP  QSTVLPDPS+F SP LLS+PLP++SFFQNFTL NGDQPEYIHPYLIKSSL
Sbjct: 61  PNFPFLFPKAQSTVLPDPSLFLSPSLLSSPLPTSSFFQNFTLKNGDQPEYIHPYLIKSSL 120

Query: 121 SSISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNL 180
           SS+SVSYPS   NS+   Q+F  DLTIS + K +P P KSHVISS++DLSVTLD+PSSN 
Sbjct: 121 SSLSVSYPSQSHNSSFIYQVFVVDLTISATNKTDPNPGKSHVISSYSDLSVTLDMPSSNF 180

Query: 181 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPIN 240
           RF+LVRGSPFLT  ++     +ISTIH ++SFS +N+LTKYT+KL NNQTWLIYSS PIN
Sbjct: 181 RFFLVRGSPFLTCLITGNTEITISTIHAILSFSSSNSLTKYTVKLNNNQTWLIYSSSPIN 240

Query: 241 LTHNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWET 300
           L+H+LS ITS GF+GIIRIA LP+SD + E ILDRFSSCYP+ G+A  TKPFCLEY+WE 
Sbjct: 241 LSHDLSSITSEGFSGIIRIAVLPDSDPKYEAILDRFSSCYPISGDASFTKPFCLEYRWEK 300

Query: 301 KGWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWH 360
           KGWGDLLMLAHPLHLRLL   D +V +LD FKY SIDGELVGVVG SW LK +P+SV+WH
Sbjct: 301 KGWGDLLMLAHPLHLRLLSSKDSDVTVLDDFKYNSIDGELVGVVGDSWVLKTDPVSVTWH 360

Query: 361 SIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLE 420
           SI+G++EES+ EI+ AL KDVE L+S++  +TT S Y YGKLIARAARLA+IAEEV  L+
Sbjct: 361 SIKGIKEESYGEIVDALVKDVEGLDSSA--ITTTSSYFYGKLIARAARLALIAEEVNFLD 420

Query: 421 MVPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHL 480
           ++P IRKFL   I+PWL GTF GNGF++D+KWGGIV+K+G  D  ADFGFG+YN+HH+HL
Sbjct: 421 VIPTIRKFLKETIDPWLEGTFGGNGFLHDDKWGGIVTKQGLTDSGADFGFGIYNDHHYHL 480

Query: 481 GYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTG 540
           GYFLY IAVL K+DPAWGRKY  Q YSLMAD MNL RR+NS + +LRCFD YKLHSW  G
Sbjct: 481 GYFLYGIAVLAKVDPAWGRKYRSQAYSLMADFMNLGRRSNSNYTRLRCFDLYKLHSWAGG 540

Query: 541 LSEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGE 600
           L+EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+ GS+LAALEI A Q WW ++EG+
Sbjct: 541 LTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSMLAALEIHAAQTWWHVKEGD 600

Query: 601 TTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFV 660
             LY E+F  ENR+VGVLW++KRDSGLWFAP  WKECRLGIQ+LP+LPI+E+L SDVGFV
Sbjct: 601 -KLYTEDFTGENRLVGVLWASKRDSGLWFAPPAWKECRLGIQLLPLLPISEVLFSDVGFV 660

Query: 661 RELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVH 720
           R+LVNW LP+LGREGVGEGWKGFV+ALE IYDK+ +L+KI+NL + DDGN+LTNLLWW+H
Sbjct: 661 RKLVNWTLPALGREGVGEGWKGFVYALEGIYDKEIALEKIKNLNDHDDGNTLTNLLWWIH 720

Query: 721 SRGKEEQ 727
           SRG EE+
Sbjct: 721 SRGDEEE 722

BLAST of Cucsa.238230 vs. TrEMBL
Match: A0A061EHI7_THECC (Glycosyl hydrolase family 81 protein OS=Theobroma cacao GN=TCM_019610 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 3.1e-297
Identity = 493/727 (67.81%), Postives = 592/727 (81.43%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           MLKK+ R +  +I     + P+  P  P  PP  PSP PSP PP  P         KP++
Sbjct: 1   MLKKLGRKVKTLI-----TKPFKRPKKPCKPPKSPSPSPSPSPPMSPP--------KPTK 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
             PFLFP  QS+VLPDPSVFFSP+LLS PLP+NSFFQNFTL NGDQPEYIHPYLIKS+ S
Sbjct: 61  YCPFLFPGVQSSVLPDPSVFFSPKLLSTPLPTNSFFQNFTLKNGDQPEYIHPYLIKSADS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           S+S+SYPS   NS+   QIF  DLTI  +++ NP  QK+H+ISS++DLS+TLD P SNLR
Sbjct: 121 SLSISYPSHFHNSSFLYQIFISDLTIFATDRTNPTSQKNHIISSYSDLSLTLDFPCSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLT  V+     SISTIH ++SFS N +LTK+TIKL NNQTWLIY+S  I+L
Sbjct: 181 FYLVRGSPFLTCFVTGNTPISISTIHAILSFSSNTSLTKHTIKLNNNQTWLIYTSSAISL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300
            H+LS+ITSGGF+GIIRIA LP+SD   E ILD++SSCYP+ G+A +TKPFCLEYKWE K
Sbjct: 241 NHSLSLITSGGFSGIIRIAVLPDSDPTYESILDQYSSCYPISGDAVLTKPFCLEYKWEKK 300

Query: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHL +L   D  V +L+ FKYKSIDGELVG+VG SW LKP  +SV+WHS
Sbjct: 301 GWGDLLMLAHPLHLNILSRDDSEVTVLENFKYKSIDGELVGIVGDSWVLKPHQVSVTWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420
           I+GV+EES+ EI++AL KDVEAL+S++  +TT S Y YGKLIARAARLA+IAEEV  L++
Sbjct: 361 IKGVKEESYHEIVTALSKDVEALDSSA--ITTTSSYFYGKLIARAARLALIAEEVCFLDV 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480
           +P IRKFL   IEPWL+GTF GNGF+YD KWGGIV+K+G+ D  ADFGFG+YN+HH+H+G
Sbjct: 421 IPAIRKFLKDTIEPWLDGTFNGNGFLYDSKWGGIVTKQGSTDSGADFGFGIYNDHHYHIG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540
           YFLYAIAVL KIDPAWGR+Y PQ Y+L+AD MNL RR+NS +P+LRCFD YKLHSW  GL
Sbjct: 481 YFLYAIAVLAKIDPAWGRRYKPQAYTLVADFMNLGRRSNSNYPRLRCFDLYKLHSWAGGL 540

Query: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600
           +EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+ GS LAA+EI+A Q WW ++EG+ 
Sbjct: 541 TEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSTLAAMEIQAAQTWWHVKEGD- 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
            LY+EEF  ENRVVGVLW+NKRDSGLWFAP++W+ECRLGIQ+LPILPI+E+L SD+ F R
Sbjct: 601 NLYEEEFTTENRVVGVLWANKRDSGLWFAPADWRECRLGIQLLPILPISEVLFSDISFTR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNW LP+LGREGVGEGWKGFV+ALE+IYDK+ +L+KIRNL  +DDGNSLTNLLWWVHS
Sbjct: 661 ELVNWTLPALGREGVGEGWKGFVYALEAIYDKESALEKIRNLNGYDDGNSLTNLLWWVHS 711

Query: 721 RGKEEQN 728
           RG E ++
Sbjct: 721 RGDEHED 711

BLAST of Cucsa.238230 vs. TAIR10
Match: AT5G15870.1 (AT5G15870.1 glycosyl hydrolase family 81 protein)

HSP 1 Score: 933.7 bits (2412), Expect = 6.9e-272
Identity = 456/732 (62.30%), Postives = 572/732 (78.14%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           MLKK+ R + +V+  K    P   PP PPPP P P P PSPPP Q       + S    +
Sbjct: 1   MLKKVRRKV-KVLITKPFKKPKNRPPSPPPPLPLP-PSPSPPPSQ-------QMSSSRQK 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
            TPFLFP + S+VLPDPS FFS +LLS+PLP+NSFFQNFTLNNGDQ EY HPY+IK S S
Sbjct: 61  NTPFLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           S+S+SYPS+  NSA   + F  D+TI+ S+  +P  +KSH+ISSF+DL VTLD PSSNLR
Sbjct: 121 SLSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           F+LVRGSPF+TF+V+  +  +ISTIH V+S S N + TKYT+KL NNQTWLIY+S PINL
Sbjct: 181 FFLVRGSPFITFSVNSSI--TISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINL 240

Query: 241 THN-LSMITSG-GFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWE 300
           T + +S I  G GF+GIIRI  LPN +   E ILD FS  YPV G+A  TKPF LEYKWE
Sbjct: 241 TKDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWE 300

Query: 301 TKGWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSW 360
            +G+GDLLMLAHPLHL+LL  +D ++ +LD FKY SIDG+LVGV+G SW LKP+P+SV+W
Sbjct: 301 KRGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTW 360

Query: 361 HSIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSL 420
           HSI+GV+E+S  EIISAL KDV AL+S++ +  T S Y Y KLIARAARLA+IAEEV  L
Sbjct: 361 HSIKGVQEDSHQEIISALIKDVNALDSSAEV--TNSSYFYAKLIARAARLALIAEEVCYL 420

Query: 421 EMVPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHH 480
           +++P+IR +L   IEPWLNG+F  NGF+YD KWGG+++K G+ D  ADFGFG+YN+HH+H
Sbjct: 421 DVIPKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYH 480

Query: 481 LGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSR---RANSKFPKLRCFDAYKLHS 540
           LGYF+YAIAVL KIDP WG++Y PQ Y+LMAD + L +   ++NS +P+LRCFD +KLHS
Sbjct: 481 LGYFVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHS 540

Query: 541 WGTGLSEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQI 600
           W  GL+EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+  S++  LEI A +MWWQ+
Sbjct: 541 WAGGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQV 600

Query: 601 REGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSD 660
           +E +  +Y ++F  ENRVVGVLWS KRDSGLWFAP EWKECRLGIQ+LP+LP++E+L SD
Sbjct: 601 KE-DDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSD 660

Query: 661 VGFVRELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLL 720
           V FV++LVNW +P+L R+ VGEGWKGFV+ALES+YDKDG+++KI+ L  FDDGNSL+NLL
Sbjct: 661 VTFVKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLL 718

Query: 721 WWVHSRGKEEQN 728
           WWVHSR  ++ +
Sbjct: 721 WWVHSRNNDDDD 718

BLAST of Cucsa.238230 vs. TAIR10
Match: AT1G18310.1 (AT1G18310.1 glycosyl hydrolase family 81 protein)

HSP 1 Score: 797.0 bits (2057), Expect = 1.0e-230
Identity = 400/684 (58.48%), Postives = 494/684 (72.22%), Query Frame = 1

Query: 64  FLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSIS 123
           FLFP T+S+VLPDPS FFSP+LLS PLP+NSFFQNFTL NGDQ EY HPYLIKS  SS+ 
Sbjct: 8   FLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLC 67

Query: 124 VSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYL 183
           +SYPS+  NS    ++FT D+ IS S    P  +K+H+ISSF+DL VTLD PSSNLRF+L
Sbjct: 68  ISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFL 127

Query: 184 VRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHN 243
           VRGSP                          ++ TK++ KL NNQ WLIY+S PI+LT +
Sbjct: 128 VRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKH 187

Query: 244 --LSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETKG 303
              S+   GGF GI+RI  LP S+   E  LDRFSSCYPV G+A  TKPF L+Y WE +G
Sbjct: 188 GDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRG 247

Query: 304 WGDLLMLAHPLHLRLL--------------------GGSDDNVVILDKFKYKSIDGELVG 363
            GDLLMLAHPLHL+LL                    G ++ +V +LD F+YKSIDG+LVG
Sbjct: 248 SGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVG 307

Query: 364 VVGSSWALKPEPISVSWHSIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKL 423
           VVG SW LKP+ +SV+WHS++GV+++S+ EIISAL KDV  LNS++ +  T S Y YGKL
Sbjct: 308 VVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEV--TSSSYFYGKL 367

Query: 424 IARAARLAVIAEEVRSLEMVPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAF 483
           IARAAR A+IAEEV  L+++P+I  +L   IEPWL+G+F+ NGF+YD KWGG+++K+G+ 
Sbjct: 368 IARAARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSK 427

Query: 484 DYSADFGFGMYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRR---- 543
           D  ADFGFG+YN+HH+H+GYFLYAIAVL K DP WG +Y  Q YSL+AD M   R+    
Sbjct: 428 DSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNN 487

Query: 544 ANSKFPKLRCFDAYKLHSWGTGLSEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSI 603
           +NS +P+LR FD +KLHSW  GL+EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV  
Sbjct: 488 SNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVET 547

Query: 604 GSILAALEIKAGQMWWQIREGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECR 663
            S +  LEI A +MWWQ+++GE  LY ++F  ENRVVGVLWS KRDS LWF P EWKECR
Sbjct: 548 ASTIMTLEIHAAKMWWQVKKGE-ALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECR 607

Query: 664 LGIQVLPILPITELLLSDVGFVRELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQ 722
           LGIQ+LPILP              LVNW LP+L R GVGEGWKGF++ALES+YDKDG+++
Sbjct: 608 LGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIK 648

BLAST of Cucsa.238230 vs. TAIR10
Match: AT3G22800.1 (AT3G22800.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-13
Identity = 39/95 (41.05%), Postives = 47/95 (49.47%), Query Frame = 1

Query: 19  SPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVL--PD 78
           SPP   PPPPPPPPPPP PPP PPPP PP    P P   P  P P+++P      +  P 
Sbjct: 378 SPPPPPPPPPPPPPPPPPPPPPPPPPPPPPYVYPSPPPPPPSPPPYVYPPPPPPYVYPPP 437

Query: 79  PSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIH 112
           PS    P +   P PS   +   +    D P  +H
Sbjct: 438 PS---PPYVYPPPPPSPQPYMYPSPPCNDLPTPVH 469

BLAST of Cucsa.238230 vs. TAIR10
Match: AT1G12040.1 (AT1G12040.1 leucine-rich repeat/extensin 1)

HSP 1 Score: 73.2 bits (178), Expect = 7.7e-13
Identity = 45/143 (31.47%), Postives = 62/143 (43.36%), Query Frame = 1

Query: 20  PPWCLP-PPPPPP-PPPPSPPPSPPPP----QPPESQPPEPS--------QKPSEPTPFL 79
           PP+    PPPPPP PPPP P  SPPPP     P    PP PS        Q P  P+P  
Sbjct: 515 PPYVYSSPPPPPPSPPPPCPESSPPPPVVYYAPVTQSPPPPSPVYYPPVTQSPPPPSPVY 574

Query: 80  FPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVS 139
           +P   ++  P   V++ P   S P PS  ++   T +        +P +  S      V 
Sbjct: 575 YPPVTNSPPPPSPVYYPPVTYSPPPPSPVYYPQVTPSPPPPSPLYYPPVTPSPPPPSPVY 634

Query: 140 YPSILSNSASECQIFTPDLTISP 149
           YP +  +      ++ P +T SP
Sbjct: 635 YPPVTPSPPPPSPVYYPPVTPSP 657

HSP 2 Score: 62.0 bits (149), Expect = 1.8e-09
Identity = 49/164 (29.88%), Postives = 68/164 (41.46%), Query Frame = 1

Query: 16  KRHSPPWCLPPP-----------PPPPPPPPSPPP-----SPPPP----QPP------ES 75
           + +SPP   PPP           PPPPPP PSPPP     SPPPP     PP       S
Sbjct: 437 RAYSPP---PPPYSKMSPSVRAYPPPPPPSPSPPPPYVYSSPPPPYVYSSPPPPPYVYSS 496

Query: 76  QPPEPSQKPSEPTPFLF-----PSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNG 135
            PP P    S P P+++     P   S+  P P     P   S+P P   ++   T +  
Sbjct: 497 PPPPPYVYSSPPPPYVYSSPPPPYVYSSPPPPPPSPPPPCPESSPPPPVVYYAPVTQSPP 556

Query: 136 DQPEYIHPYLIKSSLSSISVSYPSILSNSASECQIFTPDLTISP 149
                 +P + +S      V YP + ++      ++ P +T SP
Sbjct: 557 PPSPVYYPPVTQSPPPPSPVYYPPVTNSPPPPSPVYYPPVTYSP 597

HSP 3 Score: 53.9 bits (128), Expect = 4.8e-07
Identity = 28/73 (38.36%), Postives = 36/73 (49.32%), Query Frame = 1

Query: 26  PPPPPPPPPPSPP--PSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLPDPSVFFSP 85
           P PPPP P   PP  PSPPPP P    P  PS  P  P+P  +P    +  P   V++  
Sbjct: 610 PSPPPPSPLYYPPVTPSPPPPSPVYYPPVTPS--PPPPSPVYYPPVTPSPPPPSPVYYPS 669

Query: 86  ELLSAPLPSNSFF 97
           E  S P P+  ++
Sbjct: 670 ETQSPPPPTEYYY 680

HSP 4 Score: 52.4 bits (124), Expect = 1.4e-06
Identity = 29/72 (40.28%), Postives = 35/72 (48.61%), Query Frame = 1

Query: 20  PPWCLPPPPPPPPPPPSPP--PSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLPDP 79
           PP  + P PPPP P   PP  PSPPPP P    P  PS  P  P+P  +PS   +  P  
Sbjct: 621 PP--VTPSPPPPSPVYYPPVTPSPPPPSPVYYPPVTPS--PPPPSPVYYPSETQSPPPPT 680

Query: 80  SVFFSPELLSAP 90
             ++SP     P
Sbjct: 681 EYYYSPSQSPPP 688

BLAST of Cucsa.238230 vs. TAIR10
Match: AT1G49750.1 (AT1G49750.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 72.0 bits (175), Expect = 1.7e-12
Identity = 38/94 (40.43%), Postives = 48/94 (51.06%), Query Frame = 1

Query: 25  PPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSEPTPFLFPSTQSTVLPDPSVFFSPE 84
           PPPPPP PPPPSPPP PPPP PP S PP     P +  P   P  Q +  P P + F+  
Sbjct: 64  PPPPPPCPPPPSPPPCPPPPSPPPSPPPPQLPPPPQLPPPAPPKPQPSP-PTPDLPFASS 123

Query: 85  LLSAPLPSNSFFQNFT----LNNGDQPEYIHPYL 115
           LL    P    F++      L + + P+  + YL
Sbjct: 124 LLKKVYPVLQRFKDLVADDKLKSWEGPDICNKYL 156

HSP 2 Score: 65.1 bits (157), Expect = 2.1e-10
Identity = 44/129 (34.11%), Postives = 59/129 (45.74%), Query Frame = 1

Query: 3   KKIERLLGRVIKGKRHSPPWCLPP---------PPPPPPP----PPSPPPSPPPPQPPES 62
           K +E ++G    G  ++PP    P         PPPPPPP    PPSPPP PPPP PP S
Sbjct: 32  KSLEIIIGG---GNDNNPPPSPSPEPEPEPADCPPPPPPPPCPPPPSPPPCPPPPSPPPS 91

Query: 63  QPPEPSQKPSEPTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFT----LNNGD 115
            PP     P +  P   P  Q +  P P + F+  LL    P    F++      L + +
Sbjct: 92  PPPPQLPPPPQLPPPAPPKPQPSP-PTPDLPFASSLLKKVYPVLQRFKDLVADDKLKSWE 151

BLAST of Cucsa.238230 vs. NCBI nr
Match: gi|778707571|ref|XP_011656028.1| (PREDICTED: endo-1,3(4)-beta-glucanase 2-like [Cucumis sativus])

HSP 1 Score: 1503.8 bits (3892), Expect = 0.0e+00
Identity = 736/736 (100.00%), Postives = 736/736 (100.00%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE
Sbjct: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR
Sbjct: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300
           THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420
           IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480
           VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540

Query: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600
           SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET
Sbjct: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
           TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEEQNINNSKIHLV 737
           RGKEEQNINNSKIHLV
Sbjct: 721 RGKEEQNINNSKIHLV 736

BLAST of Cucsa.238230 vs. NCBI nr
Match: gi|659119014|ref|XP_008459428.1| (PREDICTED: endo-1,3(4)-beta-glucanase 1-like [Cucumis melo])

HSP 1 Score: 1425.6 bits (3689), Expect = 0.0e+00
Identity = 703/736 (95.52%), Postives = 714/736 (97.01%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           M KKIER L R  KGKRHSPP  LPPPPPPPPPPP         Q PESQPPEPSQKPSE
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPP---------QTPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           SISVSYPSILS  ASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR
Sbjct: 121 SISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300
           THNLSMITSGGFAGIIRIAALPNSDLECE+ILDRFSSCYPVLGEAQ TKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHLRLL GSDDNV+ILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420
           IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLE+
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEV 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480
           VPEIRKFLIGAIEPWLNGTFEGNGF+YDEKWGGIV+KEGAFD+SADFGFG+YNNHHHHLG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGL 540

Query: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600
           +EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGS+LAALEIKAGQMWWQIREGET
Sbjct: 541 AEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGET 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
           TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEEQNINNSKIHLV 737
           RGKEE+NI+NSKIHLV
Sbjct: 721 RGKEEENISNSKIHLV 727

BLAST of Cucsa.238230 vs. NCBI nr
Match: gi|703103793|ref|XP_010097822.1| (Endo-1,3(4)-beta-glucanase 1 [Morus notabilis])

HSP 1 Score: 1036.6 bits (2679), Expect = 2.1e-299
Identity = 503/739 (68.06%), Postives = 595/739 (80.51%), Query Frame = 1

Query: 1   MLKKIER----LLGRVIKGKRHSPPWCLPPPPPPPP-PPPSPPPSPPPPQPPESQPPEPS 60
           MLKK+ R    L+ +  K  R  P +  P P  P   P PS P     P+PP+       
Sbjct: 1   MLKKLRRRVKTLVTKTFKKPRRRPEFSTPSPSTPVEVPSPSTPEEVVEPEPPKMSLSSSF 60

Query: 61  QKPSEPTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLI 120
           QK S   PFLFP  QSTV+ DPS+FFSP+LLS PLP+NSFFQNF L NGDQPEYIHPYL+
Sbjct: 61  QKRSN-APFLFPKAQSTVVSDPSLFFSPDLLSTPLPTNSFFQNFVLKNGDQPEYIHPYLV 120

Query: 121 KSSLSSISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIP 180
           KSS SS+SV YPS LSNSA   QIF  DLT+S +EK  P  QK H+ISSFNDLS+TLDIP
Sbjct: 121 KSSNSSLSVCYPSRLSNSAFLYQIFNADLTVSAAEKTAPDSQKPHLISSFNDLSLTLDIP 180

Query: 181 SSNLRFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSS 240
           SSNLRF+LVRGSPFLT T S     SISTIH ++SFS NN+ TKYT+KL NNQTWLIY+S
Sbjct: 181 SSNLRFFLVRGSPFLTCTASAKTEISISTIHAILSFSSNNSQTKYTVKLNNNQTWLIYTS 240

Query: 241 FPINLTHNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEY 300
            PI+ +H+LS++TS GF+GI+RIA LP+S+ E E ILDRFSSCYPV GE   TKPFCLEY
Sbjct: 241 SPISFSHSLSLLTSNGFSGILRIAILPDSNPEFEAILDRFSSCYPVSGEVSFTKPFCLEY 300

Query: 301 KWETKGWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPIS 360
           KWE KGWGDLLMLAHPLHL+LL G D  V +L++FKYKSIDG+LVGVVG SW LKP+P+S
Sbjct: 301 KWEKKGWGDLLMLAHPLHLKLLKGDDRGVTVLEEFKYKSIDGDLVGVVGDSWTLKPDPVS 360

Query: 361 VSWHSIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEV 420
           V+WHSI GV+EE++ EI++AL+KDVEAL++ +  +TT + Y YGKLIARAAR A+IAEEV
Sbjct: 361 VTWHSIEGVKEEAYEEIVAALQKDVEALDAAA--ITTNASYFYGKLIARAARFALIAEEV 420

Query: 421 RSLEMVPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNH 480
           R  +++P+IRKFL  AIEPWL+GTF GNGF+Y+EKWGGI++K+G  D  ADFGFG+YN+H
Sbjct: 421 RFFDVIPKIRKFLKEAIEPWLDGTFNGNGFLYEEKWGGIITKQGQGDGGADFGFGIYNDH 480

Query: 481 HHHLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHS 540
           H HLG+FLYAIAVL KIDPAWGRKY PQ YS+MAD MNLSRRA S +P+LRCFD YKLHS
Sbjct: 481 HFHLGHFLYAIAVLAKIDPAWGRKYKPQAYSMMADFMNLSRRAKSNYPRLRCFDLYKLHS 540

Query: 541 WGTGLSEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQI 600
           W  GL+EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+IGS+L ALEI+A QMWW +
Sbjct: 541 WAGGLTEFADGRNQESSSEAVNAYYSAALMGLAYGDTHLVAIGSLLTALEIQAAQMWWHV 600

Query: 601 REGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSD 660
           +EG+  LY E+F +ENRVVGVLWSNKRDSGLWFAP+ W+ECRLGIQVLP+LPITE+L SD
Sbjct: 601 KEGD-KLYDEDFTRENRVVGVLWSNKRDSGLWFAPAAWRECRLGIQVLPLLPITEVLFSD 660

Query: 661 VGFVRELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLL 720
           VGF RELVNW LP+L REGVGEGWKGFV+ALE IYDK+ +LQKI++L  FDDGNSLTNLL
Sbjct: 661 VGFTRELVNWTLPALQREGVGEGWKGFVYALEGIYDKETALQKIKSLNGFDDGNSLTNLL 720

Query: 721 WWVHSRGKEEQNINNSKIH 735
           WW+HSR +E++       H
Sbjct: 721 WWIHSRDQEKEEFGGGGKH 735

BLAST of Cucsa.238230 vs. NCBI nr
Match: gi|566165928|ref|XP_002305966.2| (hypothetical protein POPTR_0004s10500g [Populus trichocarpa])

HSP 1 Score: 1029.2 bits (2660), Expect = 3.4e-297
Identity = 497/727 (68.36%), Postives = 590/727 (81.16%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPP-SPPPPQPPESQPPEPSQKPS 60
           MLKK++R +   I+     P   +P  PP P P PSPPP SP PPQ P    P+ S    
Sbjct: 1   MLKKLKRRVLTSIRKSFRKPRKRMPYKPPTPSPSPSPPPQSPSPPQTPTM--PQHSHHRP 60

Query: 61  EPTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSL 120
              PFLFP  QSTVLPDPS+F SP LLS+PLP++SFFQNFTL NGDQPEYIHPYLIKSSL
Sbjct: 61  PNFPFLFPKAQSTVLPDPSLFLSPSLLSSPLPTSSFFQNFTLKNGDQPEYIHPYLIKSSL 120

Query: 121 SSISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNL 180
           SS+SVSYPS   NS+   Q+F  DLTIS + K +P P KSHVISS++DLSVTLD+PSSN 
Sbjct: 121 SSLSVSYPSQSHNSSFIYQVFVVDLTISATNKTDPNPGKSHVISSYSDLSVTLDMPSSNF 180

Query: 181 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPIN 240
           RF+LVRGSPFLT  ++     +ISTIH ++SFS +N+LTKYT+KL NNQTWLIYSS PIN
Sbjct: 181 RFFLVRGSPFLTCLITGNTEITISTIHAILSFSSSNSLTKYTVKLNNNQTWLIYSSSPIN 240

Query: 241 LTHNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWET 300
           L+H+LS ITS GF+GIIRIA LP+SD + E ILDRFSSCYP+ G+A  TKPFCLEY+WE 
Sbjct: 241 LSHDLSSITSEGFSGIIRIAVLPDSDPKYEAILDRFSSCYPISGDASFTKPFCLEYRWEK 300

Query: 301 KGWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWH 360
           KGWGDLLMLAHPLHLRLL   D +V +LD FKY SIDGELVGVVG SW LK +P+SV+WH
Sbjct: 301 KGWGDLLMLAHPLHLRLLSSKDSDVTVLDDFKYNSIDGELVGVVGDSWVLKTDPVSVTWH 360

Query: 361 SIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLE 420
           SI+G++EES+ EI+ AL KDVE L+S++  +TT S Y YGKLIARAARLA+IAEEV  L+
Sbjct: 361 SIKGIKEESYGEIVDALVKDVEGLDSSA--ITTTSSYFYGKLIARAARLALIAEEVNFLD 420

Query: 421 MVPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHL 480
           ++P IRKFL   I+PWL GTF GNGF++D+KWGGIV+K+G  D  ADFGFG+YN+HH+HL
Sbjct: 421 VIPTIRKFLKETIDPWLEGTFGGNGFLHDDKWGGIVTKQGLTDSGADFGFGIYNDHHYHL 480

Query: 481 GYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTG 540
           GYFLY IAVL K+DPAWGRKY  Q YSLMAD MNL RR+NS + +LRCFD YKLHSW  G
Sbjct: 481 GYFLYGIAVLAKVDPAWGRKYRSQAYSLMADFMNLGRRSNSNYTRLRCFDLYKLHSWAGG 540

Query: 541 LSEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGE 600
           L+EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+ GS+LAALEI A Q WW ++EG+
Sbjct: 541 LTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSMLAALEIHAAQTWWHVKEGD 600

Query: 601 TTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFV 660
             LY E+F  ENR+VGVLW++KRDSGLWFAP  WKECRLGIQ+LP+LPI+E+L SDVGFV
Sbjct: 601 -KLYTEDFTGENRLVGVLWASKRDSGLWFAPPAWKECRLGIQLLPLLPISEVLFSDVGFV 660

Query: 661 RELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVH 720
           R+LVNW LP+LGREGVGEGWKGFV+ALE IYDK+ +L+KI+NL + DDGN+LTNLLWW+H
Sbjct: 661 RKLVNWTLPALGREGVGEGWKGFVYALEGIYDKEIALEKIKNLNDHDDGNTLTNLLWWIH 720

Query: 721 SRGKEEQ 727
           SRG EE+
Sbjct: 721 SRGDEEE 722

BLAST of Cucsa.238230 vs. NCBI nr
Match: gi|590653358|ref|XP_007033400.1| (Glycosyl hydrolase family 81 protein [Theobroma cacao])

HSP 1 Score: 1028.9 bits (2659), Expect = 4.5e-297
Identity = 493/727 (67.81%), Postives = 592/727 (81.43%), Query Frame = 1

Query: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60
           MLKK+ R +  +I     + P+  P  P  PP  PSP PSP PP  P         KP++
Sbjct: 1   MLKKLGRKVKTLI-----TKPFKRPKKPCKPPKSPSPSPSPSPPMSPP--------KPTK 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
             PFLFP  QS+VLPDPSVFFSP+LLS PLP+NSFFQNFTL NGDQPEYIHPYLIKS+ S
Sbjct: 61  YCPFLFPGVQSSVLPDPSVFFSPKLLSTPLPTNSFFQNFTLKNGDQPEYIHPYLIKSADS 120

Query: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           S+S+SYPS   NS+   QIF  DLTI  +++ NP  QK+H+ISS++DLS+TLD P SNLR
Sbjct: 121 SLSISYPSHFHNSSFLYQIFISDLTIFATDRTNPTSQKNHIISSYSDLSLTLDFPCSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLT  V+     SISTIH ++SFS N +LTK+TIKL NNQTWLIY+S  I+L
Sbjct: 181 FYLVRGSPFLTCFVTGNTPISISTIHAILSFSSNTSLTKHTIKLNNNQTWLIYTSSAISL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300
            H+LS+ITSGGF+GIIRIA LP+SD   E ILD++SSCYP+ G+A +TKPFCLEYKWE K
Sbjct: 241 NHSLSLITSGGFSGIIRIAVLPDSDPTYESILDQYSSCYPISGDAVLTKPFCLEYKWEKK 300

Query: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHL +L   D  V +L+ FKYKSIDGELVG+VG SW LKP  +SV+WHS
Sbjct: 301 GWGDLLMLAHPLHLNILSRDDSEVTVLENFKYKSIDGELVGIVGDSWVLKPHQVSVTWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420
           I+GV+EES+ EI++AL KDVEAL+S++  +TT S Y YGKLIARAARLA+IAEEV  L++
Sbjct: 361 IKGVKEESYHEIVTALSKDVEALDSSA--ITTTSSYFYGKLIARAARLALIAEEVCFLDV 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480
           +P IRKFL   IEPWL+GTF GNGF+YD KWGGIV+K+G+ D  ADFGFG+YN+HH+H+G
Sbjct: 421 IPAIRKFLKDTIEPWLDGTFNGNGFLYDSKWGGIVTKQGSTDSGADFGFGIYNDHHYHIG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540
           YFLYAIAVL KIDPAWGR+Y PQ Y+L+AD MNL RR+NS +P+LRCFD YKLHSW  GL
Sbjct: 481 YFLYAIAVLAKIDPAWGRRYKPQAYTLVADFMNLGRRSNSNYPRLRCFDLYKLHSWAGGL 540

Query: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600
           +EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+ GS LAA+EI+A Q WW ++EG+ 
Sbjct: 541 TEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSTLAAMEIQAAQTWWHVKEGD- 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
            LY+EEF  ENRVVGVLW+NKRDSGLWFAP++W+ECRLGIQ+LPILPI+E+L SD+ F R
Sbjct: 601 NLYEEEFTTENRVVGVLWANKRDSGLWFAPADWRECRLGIQLLPILPISEVLFSDISFTR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNW LP+LGREGVGEGWKGFV+ALE+IYDK+ +L+KIRNL  +DDGNSLTNLLWWVHS
Sbjct: 661 ELVNWTLPALGREGVGEGWKGFVYALEAIYDKESALEKIRNLNGYDDGNSLTNLLWWVHS 711

Query: 721 RGKEEQN 728
           RG E ++
Sbjct: 721 RGDEHED 711