Cucsa.126580 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.126580
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionSphingolipid delta(4)-desaturase DES1-like
Locationscaffold01017 : 484699 .. 487264 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGGCAATGGCACCGATATGAAGACGGCGATGTATACAACAGCGTCCAGTCGAACCCAACGTCCTTTTCTATATTTAGGAGTCATCTTCCATTTCATTTTCCTTTCCATATTTGGTCTTCTTCAATCCTCCGCCCATTCTTAAATCTTCAACAACCTTCCTCTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCAATCCACATCCAATTTCATCAGCACCAGCCCCCAGGAGACGCAATTTCTTCCGGAGCATCTTCTGGGAGCAACcCCCcACCCCGGGAATCACTCACAAACACAATGCCAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTTTtTtCTTTtttTTTttAATTTCTTCTCTTTCTTGATTCTGAGAATGTAGAGAGTTGTGGTTATTTTCTTGTCAGGATGTTTTCATTTCGTGTTTAGATCTGTATTTCTGGTCTCTACACATGTCAATGCCTGAATTTGATCTGTGTATATTTGTTTTTATGTTTGATTTTGAACCTCATTTTTGCTACATTGTTGCGTTTTACTTTGTTTTtGTTTTtCTTTTATCATTCTGATTACTCGTGTTTGTTGCATCCATCGAAAATCGACATTTGAGATTCTGAACGCTTGTTATTGATGGATCTGTCCACTGCGCTGTTCTTTTTtGCTCTGGTTCAGTTATTTTTTtGCTACATTCCCATATCGTTTATTTATTCGTGTGTttCTTCCTTTCTTCCTTCTTTGTATTACCAACGCCTGTCTAAATTTTCCTGCTTTTTCAACTATTAGGCGAGCTTTGATTCAGGATTGTTTTGGTTTAAACTTTATTTAAAGTTATCCTTCTTTTCTTATTTGGAATTTAAAAGAATGCCCTGGAGGAATTTAACCCTCAACCTTTTTCACTAGGAAATGGTAAAGTTTAGTAGTACTCGATGAATTTTATTGTTGTTTGCTCTCTAGAGTTTTGGTAAATGTTTAGAGAGGAAATTAGCCCTCGACCCTTATTTCCTTGGTTGCCTGGCTCTAATATCTGATTTTAGCAGATGTTTAATCGTTTGATTAGGGTAGTCCATTGCAGTAGTTTATGGCTTGTATAGTTTCAAATGAGACCATTTATGATAATCAGAAGAATAAATTGTTGAAGTTAAATATTTAGACTAATGCTTCCTGCTGTTGTGAAGGGAAGAACTTTTTTTTTtAATCGTTTGTTGTATTTGTTAAGAAGAGCAAGTGATTGTAATTATTTAACCAACTGCATTGGCATTTTGATTCATCTGATGAAAAAAAaTAAaGTCATGATCACGAAGAATGAGAACTTGGTGTGAACAGCCATTTAACATTCACGAACCTGACCTTGTCGTCATAAAGTTTAGCTATTGTAGTTTGGAGAACCAATCATTAATTTATTCTTACAGTATAAAAAGTTGGTCACTTTTTTCTTCTCGTTCTCATTCTTGCAATCTTGCAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTTGAGCACCATCGCTTTCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTATTCTTCTATGCACTCCGCCCACTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCCCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACTTGATCCTCTCCACATTCGTTGGCGGTGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAAACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTATAAAGTGAAGGAGATTGCACCTGAATACTACAACGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAGTTCATCCTTTCTCCTCCTCTCCCCTTTTAGATATTTTTTGTTCGATTCTTTCTCCACCTTTTCTGGTGGATCATTCTCTTTGAGCTGGTTGTGCCTTTGTTTTATTTACATACATTCATGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCCAGGCA

mRNA sequence

GTTGGCAATGGCACCGATATGAAGACGGCGATGTATACAACAGCGTCCAGTCGAACCCAACGTCCTTTTCTATATTTAGGAGTCATCTTCCATTTCATTTTCCTTTCCATATTTGGTCTTCTTCAATCCTCCGCCCATTCTTAAATCTTCAACAACCTTCCTCTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCAATCCACATCCAATTTCATCAGCACCAGCCCCCAGGAGACGCAATTTCTTCCGGAGCATCTTCTGGGAGCAACCCCCCACCCCGGGAATCACTCACAAACACAATGCCAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTTGAGCACCATCGCTTTCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTATTCTTCTATGCACTCCGCCCACTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCCCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACTTGATCCTCTCCACATTCGTTGGCGGTGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAAACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTATAAAGTGAAGGAGATTGCACCTGAATACTACAACGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAGTTCATCCTTTCTCCTCCTCTCCCCTTTTAGATATTTTTTGTTCGATTCTTTCTCCACCTTTTCTGGTGGATCATTCTCTTTGAGCTGGTTGTGCCTTTGTTTTATTTACATACATTCATGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCCAGGCA

Coding sequence (CDS)

ATGCCAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTTGAGCACCATCGCTTTCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTATTCTTCTATGCACTCCGCCCACTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCCCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACTTGATCCTCTCCACATTCGTTGGCGGTGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAAACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTATAAAGTGAAGGAGATTGCACCTGAATACTACAACGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAG

Protein sequence

MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE*
Homology
[Close]
BLAST of Cucsa.126580 vs. Swiss-Prot
Match: DES1L_ORYSJ (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 2.2e-163
Identity = 263/322 (81.68%), Postives = 290/322 (90.06%), Query Frame = 1 

Query: 3   GGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLW 62
           G    ++GVMATDFFWSYTDEPHA+RRR+IL+++PQI+ELFGPDP AF KI+ VV LQLW
Sbjct: 6   GDGREEEGVMATDFFWSYTDEPHATRRREILAKHPQIKELFGPDPLAFLKIAAVVSLQLW 65

Query: 63  TATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGV 122
           TAT L  A W+KIL +AYFFGSFLNHNLFLAIHELSHNLAF+TP YN+WLGIFANLPIGV
Sbjct: 66  TATLLRDASWVKILTVAYFFGSFLNHNLFLAIHELSHNLAFTTPSYNRWLGIFANLPIGV 125

Query: 123 PMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLK 182
           PMSITFQKYHLEHHRFQGVDG+DMD+PSQ EAH V N +SK++WV+FQLFFYALRPLFLK
Sbjct: 126 PMSITFQKYHLEHHRFQGVDGIDMDIPSQAEAHAVKNTLSKSVWVVFQLFFYALRPLFLK 185

Query: 183 PKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 242
           PKPPG WEF N IIQIALD +MVYF+ WKS AYLILSTFVGGGMHPMAGHFISEHYVFNP
Sbjct: 186 PKPPGLWEFTNLIIQIALDASMVYFFGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 245

Query: 243 KQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIY 302
            QETYSYYGPLNL+TW VGYHNEHHDFPRIPG++LYKV+EIAPEYYN L SYKSWSQVIY
Sbjct: 246 DQETYSYYGPLNLMTWHVGYHNEHHDFPRIPGTRLYKVREIAPEYYNNLKSYKSWSQVIY 305

Query: 303 MYIMDKTVGPFSRMKRMPRKSE 325
           MYIMD+TVGPFSRMKR   K +
Sbjct: 306 MYIMDQTVGPFSRMKRKAPKKD 327

[Close]
BLAST of Cucsa.126580 vs. Swiss-Prot
Match: DES1L_ARATH (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 1.9e-162
Identity = 266/320 (83.12%), Postives = 293/320 (91.56%), Query Frame = 1 

Query: 5   EERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTA 64
           EE  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQL TA
Sbjct: 13  EEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTA 72

Query: 65  TTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPM 124
             LH++ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+ LGIFANLPIGVPM
Sbjct: 73  AILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPM 132

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPK
Sbjct: 133 SVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPK 192

Query: 185 PPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP Q
Sbjct: 193 PPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ 252

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQVIYMY
Sbjct: 253 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMY 312

Query: 305 IMDKTVGPFSRMKRMPRKSE 325
           IMD TVGP+SRMKR   KS+
Sbjct: 313 IMDTTVGPYSRMKRKLSKSD 332

[Close]
BLAST of Cucsa.126580 vs. Swiss-Prot
Match: DEGS1_MICFO (Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-96
Identity = 159/316 (50.32%), Postives = 222/316 (70.25%), Query Frame = 1 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSA 70
           V   +F W YTD+PH  RRR+IL++YP+I+ L  PDP   + I+ + L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHTDRRREILAKYPEIKSLMKPDPNLIWIITAMFLVQLISLYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD--YNKWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F      +N+W GIFANLPIGVP S++F
Sbjct: 65  DWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKAVWNRWFGIFANLPIGVPYSVSF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K +WVI Q  FYALRPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTFRKLVWVILQPLFYALRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + QI LD+ + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEIINTVTQIILDIIVYYVFGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY++L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIAAEYYDKLPQYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ SE
Sbjct: 305 TISPYSRMKRPPKGSE 320

[Close]
BLAST of Cucsa.126580 vs. Swiss-Prot
Match: DEGS1_HUMAN (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 7.4e-95
Identity = 158/310 (50.97%), Postives = 216/310 (69.68%), Query Frame = 1 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSA 70
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP   + I ++VL QL     +   
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD--YNKWLGIFANLPIGVPMSITF 130
           DW  ++  AY FGS +NH++ LAIHE++HN AF      +N+W G+FANLPIG+P SI+F
Sbjct: 65  DWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K IWVI Q  FYA RPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + Q+  D+ + YF   KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP IPG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKR 319
           T+ P+SRMKR
Sbjct: 305 TISPYSRMKR 314

[Close]
BLAST of Cucsa.126580 vs. Swiss-Prot
Match: DEGS1_RAT (Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus GN=Degs1 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 9.7e-95
Identity = 155/316 (49.05%), Postives = 223/316 (70.57%), Query Frame = 1 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSA 70
           V   +F W YTD+PHA+RR++IL++YP+I+ L  PDP   + ++ ++L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHAARRQEILAKYPEIKSLMKPDPNLIWIVTSMLLVQLASFYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD--YNKWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F      +N+W G+FANL +GVP SI+F
Sbjct: 65  DWKWLMFWSYAFGSCLNHSMTLAIHEISHNFPFGHHKAMWNRWFGMFANLSLGVPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E       + K +WVI Q  FYA RPLF+ PKP  H
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTLRKLVWVILQPLFYAFRPLFINPKPITH 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN +IQ+  DV + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVIQVTFDVLVYYVFGVKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIASEYYDNLPHYNSWIRVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ +E
Sbjct: 305 TISPYSRMKRPPKGNE 320

[Close]
BLAST of Cucsa.126580 vs. TrEMBL
Match: A0A0A0LSB9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073750 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 7.5e-195
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 1 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cucsa.126580 vs. TrEMBL
Match: M5WTY1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008431mg PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 2.6e-171
Identity = 283/321 (88.16%), Postives = 301/321 (93.77%), Query Frame = 1 

Query: 4   GEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWT 63
           GE  ++GVMATDFFWSYTDEPHASRRRQILSQYPQI+ELFGPDPWAF KI+VVVLLQLWT
Sbjct: 12  GEAEEEGVMATDFFWSYTDEPHASRRRQILSQYPQIKELFGPDPWAFLKITVVVLLQLWT 71

Query: 64  ATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVP 123
           AT LH+A WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGIFANLPIGVP
Sbjct: 72  ATALHNAGWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGVP 131

Query: 124 MSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKP 183
           MS+TFQKYHLEHHRFQGVDG+DMD+PS+ EAHLVTNVV+KAIWV+ QLFFYA RP+FLKP
Sbjct: 132 MSVTFQKYHLEHHRFQGVDGIDMDIPSKVEAHLVTNVVNKAIWVLLQLFFYAFRPVFLKP 191

Query: 184 KPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPK 243
           KPPG WEF+N  IQIALDV+MVYF  WKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 
Sbjct: 192 KPPGCWEFLNLFIQIALDVSMVYFCGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPD 251

Query: 244 QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYM 303
           QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKL+KVK IAPEYY  L+SYKSWSQVIYM
Sbjct: 252 QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLHKVKGIAPEYYEGLESYKSWSQVIYM 311

Query: 304 YIMDKTVGPFSRMKRMPRKSE 325
           YIMD+TVGPFSRMKR P KSE
Sbjct: 312 YIMDRTVGPFSRMKRNPSKSE 332

[Close]
BLAST of Cucsa.126580 vs. TrEMBL
Match: B9RZ74_RICCO (Sphingolipid delta 4 desaturase/C-4 hydroxylase protein des2, putative OS=Ricinus communis GN=RCOM_0935980 PE=4 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 3.5e-168
Identity = 275/314 (87.58%), Postives = 296/314 (94.27%), Query Frame = 1 

Query: 5   EERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTA 64
           EE  +GVMATDFFWSYTDEPHASRRRQILSQYPQI++LFGPDP+AFFK+SVVVLLQL TA
Sbjct: 11  EEEAEGVMATDFFWSYTDEPHASRRRQILSQYPQIKQLFGPDPFAFFKVSVVVLLQLGTA 70

Query: 65  TTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPM 124
             LH + WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGIFANLP+GVPM
Sbjct: 71  ALLHDSGWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPVGVPM 130

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMD+PSQ EAHLV NV++K+IWVIFQLFFYALRPLFLKPK
Sbjct: 131 SVTFQKYHLEHHRFQGVDGIDMDIPSQIEAHLVRNVITKSIWVIFQLFFYALRPLFLKPK 190

Query: 185 PPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG+WEFIN  +QIALDVAMVYFW WKSFAYLILSTFVGGGMHPMA HFISEHYVFNP+Q
Sbjct: 191 PPGYWEFINLFVQIALDVAMVYFWGWKSFAYLILSTFVGGGMHPMAAHFISEHYVFNPEQ 250

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLN +TW VGYHNEHHDFPRIPGSKL+KVKEIAPEYY  LDSYKSWSQVIYMY
Sbjct: 251 ETYSYYGPLNFVTWHVGYHNEHHDFPRIPGSKLHKVKEIAPEYYEGLDSYKSWSQVIYMY 310

Query: 305 IMDKTVGPFSRMKR 319
           +MD+TVGPFSRMKR
Sbjct: 311 VMDRTVGPFSRMKR 324

[Close]
BLAST of Cucsa.126580 vs. TrEMBL
Match: A0A067G8D5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020224mg PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 8.6e-167
Identity = 274/324 (84.57%), Postives = 298/324 (91.98%), Query Frame = 1 

Query: 4   GEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWT 63
           GEER++GVMA DFFWSYTDEPHASRRRQILSQYPQI+ELFGPDPWAF K++VVVLLQL+T
Sbjct: 6   GEEREEGVMAADFFWSYTDEPHASRRRQILSQYPQIKELFGPDPWAFPKVTVVVLLQLYT 65

Query: 64  ATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVP 123
           AT LH A WLKILA AYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGI ANLP+GVP
Sbjct: 66  ATLLHDAGWLKILATAYFFGSFLNHNLFLAIHELSHNLAFSTPAYNRWLGILANLPVGVP 125

Query: 124 MSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKP 183
           MS+TFQKYHLEHHRFQGVDG+DMD+PS TE  +VTNVV+K+IWVIFQL FYA RP+FLKP
Sbjct: 126 MSVTFQKYHLEHHRFQGVDGIDMDIPSLTETRMVTNVVAKSIWVIFQLLFYAFRPVFLKP 185

Query: 184 KPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPK 243
           KPPG+WEFINF IQIALD+ +VYFW WKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 
Sbjct: 186 KPPGYWEFINFSIQIALDITIVYFWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPD 245

Query: 244 QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYM 303
           QETYSYYGPLN LTW VGYHNEHHDFPRIPG+KL+KV+E+APEYY  L SYKSWSQVIYM
Sbjct: 246 QETYSYYGPLNFLTWHVGYHNEHHDFPRIPGNKLHKVREVAPEYYQGLASYKSWSQVIYM 305

Query: 304 YIMDKTVGPFSRMKRMP---RKSE 325
           YIMD+TVGPFSRMKR P   +KSE
Sbjct: 306 YIMDRTVGPFSRMKRKPSATKKSE 329

[Close]
BLAST of Cucsa.126580 vs. TrEMBL
Match: F6HD78_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0583g00030 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.1e-166
Identity = 273/318 (85.85%), Postives = 295/318 (92.77%), Query Frame = 1 

Query: 3   GGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLW 62
           GGEER++GVMA DFFWSYTDEPHASRRRQILSQYPQI++LFGPDP+AF K++ VVLLQL 
Sbjct: 2   GGEEREEGVMANDFFWSYTDEPHASRRRQILSQYPQIKDLFGPDPFAFLKVAGVVLLQLG 61

Query: 63  TATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGV 122
           TAT LH A WLKIL +AYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGIFANLPIGV
Sbjct: 62  TATVLHDAGWLKILTVAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGV 121

Query: 123 PMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLK 182
           PMS+TFQKYHLEHHR+QGVDGMDMDVPSQ EA LV N ++K+IWV FQLFFYALRPLFL 
Sbjct: 122 PMSVTFQKYHLEHHRYQGVDGMDMDVPSQIEALLVRNALTKSIWVTFQLFFYALRPLFLN 181

Query: 183 PKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 242
           PKPPG WEF N I+Q+ALD AMVYFW WKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP
Sbjct: 182 PKPPGVWEFFNLIVQLALDAAMVYFWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 241

Query: 243 KQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIY 302
           +QETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLY+VKEIAPEYY +L+SYKSWSQVIY
Sbjct: 242 EQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYRVKEIAPEYYEDLESYKSWSQVIY 301

Query: 303 MYIMDKTVGPFSRMKRMP 321
           MYIMD T+GPFSRMKR P
Sbjct: 302 MYIMDCTIGPFSRMKRKP 319

[Close]
BLAST of Cucsa.126580 vs. TAIR10
Match: AT4G04930.1 (AT4G04930.1 fatty acid desaturase family protein)

HSP 1 Score: 573.2 bits (1476), Expect = 1.0e-163
Identity = 266/320 (83.12%), Postives = 293/320 (91.56%), Query Frame = 1 

Query: 5   EERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTA 64
           EE  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQL TA
Sbjct: 13  EEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTA 72

Query: 65  TTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPM 124
             LH++ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+ LGIFANLPIGVPM
Sbjct: 73  AILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPM 132

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPK
Sbjct: 133 SVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPK 192

Query: 185 PPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP Q
Sbjct: 193 PPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ 252

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQVIYMY
Sbjct: 253 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMY 312

Query: 305 IMDKTVGPFSRMKRMPRKSE 325
           IMD TVGP+SRMKR   KS+
Sbjct: 313 IMDTTVGPYSRMKRKLSKSD 332

[Close]
BLAST of Cucsa.126580 vs. NCBI nr
Match: gi|449457719|ref|XP_004146595.1| (PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus])

HSP 1 Score: 687.6 bits (1773), Expect = 1.1e-194
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 1 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cucsa.126580 vs. NCBI nr
Match: gi|659068034|ref|XP_008442400.1| (PREDICTED: sphingolipid delta(4)-desaturase DES1 [Cucumis melo])

HSP 1 Score: 679.5 bits (1752), Expect = 2.9e-192
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 1 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cucsa.126580 vs. NCBI nr
Match: gi|645237130|ref|XP_008225064.1| (PREDICTED: sphingolipid delta(4)-desaturase DES1 [Prunus mume])

HSP 1 Score: 609.8 bits (1571), Expect = 2.9e-171
Identity = 283/321 (88.16%), Postives = 301/321 (93.77%), Query Frame = 1 

Query: 4   GEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWT 63
           GE  ++GVMATDFFWSYTDEPHASRRRQILSQYPQI+ELFGPDPWAF KI+VVVLLQLWT
Sbjct: 7   GEAEEEGVMATDFFWSYTDEPHASRRRQILSQYPQIKELFGPDPWAFLKITVVVLLQLWT 66

Query: 64  ATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVP 123
           AT LH+A WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGIFANLPIGVP
Sbjct: 67  ATALHNAGWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGVP 126

Query: 124 MSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKP 183
           MS+TFQKYHLEHHRFQGVDG+DMD+PS+ EAHLVTNVV KAIWV+ QLFFYALRP+FLKP
Sbjct: 127 MSVTFQKYHLEHHRFQGVDGIDMDIPSKVEAHLVTNVVKKAIWVLLQLFFYALRPVFLKP 186

Query: 184 KPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPK 243
           KPPG+WEF+N  IQIALDV+MVYF  WKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 
Sbjct: 187 KPPGYWEFLNLFIQIALDVSMVYFCGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPD 246

Query: 244 QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYM 303
           QETYSYYGPLNLLTWSVGYHNEHHDFPRI GSKL+KVK IAPEYY  L+SYKSWSQVIYM
Sbjct: 247 QETYSYYGPLNLLTWSVGYHNEHHDFPRISGSKLHKVKGIAPEYYEGLESYKSWSQVIYM 306

Query: 304 YIMDKTVGPFSRMKRMPRKSE 325
           YIMD+TVGPFSRMKR P KSE
Sbjct: 307 YIMDRTVGPFSRMKRKPSKSE 327

[Close]
BLAST of Cucsa.126580 vs. NCBI nr
Match: gi|595863397|ref|XP_007211574.1| (hypothetical protein PRUPE_ppa008431mg [Prunus persica])

HSP 1 Score: 609.4 bits (1570), Expect = 3.7e-171
Identity = 283/321 (88.16%), Postives = 301/321 (93.77%), Query Frame = 1 

Query: 4   GEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWT 63
           GE  ++GVMATDFFWSYTDEPHASRRRQILSQYPQI+ELFGPDPWAF KI+VVVLLQLWT
Sbjct: 12  GEAEEEGVMATDFFWSYTDEPHASRRRQILSQYPQIKELFGPDPWAFLKITVVVLLQLWT 71

Query: 64  ATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVP 123
           AT LH+A WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGIFANLPIGVP
Sbjct: 72  ATALHNAGWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGVP 131

Query: 124 MSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKP 183
           MS+TFQKYHLEHHRFQGVDG+DMD+PS+ EAHLVTNVV+KAIWV+ QLFFYA RP+FLKP
Sbjct: 132 MSVTFQKYHLEHHRFQGVDGIDMDIPSKVEAHLVTNVVNKAIWVLLQLFFYAFRPVFLKP 191

Query: 184 KPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPK 243
           KPPG WEF+N  IQIALDV+MVYF  WKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 
Sbjct: 192 KPPGCWEFLNLFIQIALDVSMVYFCGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPD 251

Query: 244 QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYM 303
           QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKL+KVK IAPEYY  L+SYKSWSQVIYM
Sbjct: 252 QETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLHKVKGIAPEYYEGLESYKSWSQVIYM 311

Query: 304 YIMDKTVGPFSRMKRMPRKSE 325
           YIMD+TVGPFSRMKR P KSE
Sbjct: 312 YIMDRTVGPFSRMKRNPSKSE 332

[Close]
BLAST of Cucsa.126580 vs. NCBI nr
Match: gi|255556017|ref|XP_002519043.1| (PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Ricinus communis])

HSP 1 Score: 599.0 bits (1543), Expect = 5.0e-168
Identity = 275/314 (87.58%), Postives = 296/314 (94.27%), Query Frame = 1 

Query: 5   EERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTA 64
           EE  +GVMATDFFWSYTDEPHASRRRQILSQYPQI++LFGPDP+AFFK+SVVVLLQL TA
Sbjct: 11  EEEAEGVMATDFFWSYTDEPHASRRRQILSQYPQIKQLFGPDPFAFFKVSVVVLLQLGTA 70

Query: 65  TTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPM 124
             LH + WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+WLGIFANLP+GVPM
Sbjct: 71  ALLHDSGWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPVGVPM 130

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMD+PSQ EAHLV NV++K+IWVIFQLFFYALRPLFLKPK
Sbjct: 131 SVTFQKYHLEHHRFQGVDGIDMDIPSQIEAHLVRNVITKSIWVIFQLFFYALRPLFLKPK 190

Query: 185 PPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG+WEFIN  +QIALDVAMVYFW WKSFAYLILSTFVGGGMHPMA HFISEHYVFNP+Q
Sbjct: 191 PPGYWEFINLFVQIALDVAMVYFWGWKSFAYLILSTFVGGGMHPMAAHFISEHYVFNPEQ 250

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLN +TW VGYHNEHHDFPRIPGSKL+KVKEIAPEYY  LDSYKSWSQVIYMY
Sbjct: 251 ETYSYYGPLNFVTWHVGYHNEHHDFPRIPGSKLHKVKEIAPEYYEGLDSYKSWSQVIYMY 310

Query: 305 IMDKTVGPFSRMKR 319
           +MD+TVGPFSRMKR
Sbjct: 311 VMDRTVGPFSRMKR 324

The following BLAST results are available for this feature:
BLAST of Cucsa.126580 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DES1L_ORYSJ2.2e-16381.68Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica GN=Os... [more]
DES1L_ARATH1.9e-16283.13Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana GN=At4g04930 ... [more]
DEGS1_MICFO1.8e-9650.32Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis GN=DEGS1 PE=2 SV=1[more]
DEGS1_HUMAN7.4e-9550.97Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens GN=DEGS1 PE=1 SV=1[more]
DEGS1_RAT9.7e-9549.05Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus GN=Degs1 PE=2 SV=1[more]
BLAST of Cucsa.126580 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LSB9_CUCSA7.5e-195100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073750 PE=4 SV=1[more]
M5WTY1_PRUPE2.6e-17188.16Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008431mg PE=4 SV=1[more]
B9RZ74_RICCO3.5e-16887.58Sphingolipid delta 4 desaturase/C-4 hydroxylase protein des2, putative OS=Ricinu... [more]
A0A067G8D5_CITSI8.6e-16784.57Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020224mg PE=4 SV=1[more]
F6HD78_VITVI1.1e-16685.85Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0583g00030 PE=4 SV=... [more]
BLAST of Cucsa.126580 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G04930.11.0e-16383.13 fatty acid desaturase family protein[more]
BLAST of Cucsa.126580 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449457719|ref|XP_004146595.1|1.1e-194100.00PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus][more]
gi|659068034|ref|XP_008442400.1|2.9e-19298.77PREDICTED: sphingolipid delta(4)-desaturase DES1 [Cucumis melo][more]
gi|645237130|ref|XP_008225064.1|2.9e-17188.16PREDICTED: sphingolipid delta(4)-desaturase DES1 [Prunus mume][more]
gi|595863397|ref|XP_007211574.1|3.7e-17188.16hypothetical protein PRUPE_ppa008431mg [Prunus persica][more]
gi|255556017|ref|XP_002519043.1|5.0e-16887.58PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR011388DES1/DES2
IPR013866Sphingolipid_d4-desaturase_N
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0030148sphingolipid biosynthetic process
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Molecular Function
TermDefinition
GO:0042284sphingolipid delta-4 desaturase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009827 plant-type cell wall modification
biological_process GO:0009860 pollen tube growth
biological_process GO:0030148 sphingolipid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042284 sphingolipid delta-4 desaturase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.126580.1Cucsa.126580.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 72..290
score: 1.1
IPR011388Sphingolipid delta4-desaturasePIRPIRSF017228Sphnglp_dlt4_descoord: 4..324
score: 2.9E
IPR013866Sphingolipid delta4-desaturase, N-terminalPFAMPF08557Lipid_DEScoord: 14..48
score: 4.6
IPR013866Sphingolipid delta4-desaturase, N-terminalSMARTSM01269Lipid_DES_2coord: 11..49
score: 7.2
NoneNo IPR availablePANTHERPTHR12879SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2coord: 5..324
score: 2.0E
NoneNo IPR availablePANTHERPTHR12879:SF8DES-1 PROTEINcoord: 5..324
score: 2.0E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.126580Cla019496Watermelon (97103) v1cgywmB205
Cucsa.126580Cla009611Watermelon (97103) v1cgywmB208
Cucsa.126580Csa1G073750Cucumber (Chinese Long) v2cgycuB170
Cucsa.126580MELO3C002591Melon (DHL92) v3.5.1cgymeB198
Cucsa.126580ClCG01G022870Watermelon (Charleston Gray)cgywcgB202
Cucsa.126580ClCG03G005940Watermelon (Charleston Gray)cgywcgB203
Cucsa.126580CSPI01G12010Wild cucumber (PI 183967)cgycpiB172
Cucsa.126580CmaCh18G003620Cucurbita maxima (Rimu)cgycmaB0338
Cucsa.126580CmoCh18G003410Cucurbita moschata (Rifu)cgycmoB0332
Cucsa.126580Lsi02G007430Bottle gourd (USVL1VR-Ls)cgylsiB194
Cucsa.126580Lsi01G003250Bottle gourd (USVL1VR-Ls)cgylsiB193
Cucsa.126580Cp4.1LG09g08400Cucurbita pepo (Zucchini)cgycpeB0324
Cucsa.126580Cp4.1LG20g04200Cucurbita pepo (Zucchini)cgycpeB0328
Cucsa.126580MELO3C007292.2Melon (DHL92) v3.6.1cgymedB199
Cucsa.126580MELO3C002591.2Melon (DHL92) v3.6.1cgymedB197
Cucsa.126580CsaV3_1G012180Cucumber (Chinese Long) v3cgycucB174
Cucsa.126580Cla97C01G022770Watermelon (97103) v2cgywmbB197
Cucsa.126580Cla97C03G057250Watermelon (97103) v2cgywmbB200
Cucsa.126580Bhi03G000329Wax gourdcgywgoB230
Cucsa.126580Bhi08G001271Wax gourdcgywgoB233
Cucsa.126580Carg21494Silver-seed gourdcarcgyB1068
Cucsa.126580Carg14634Silver-seed gourdcarcgyB0182
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.126580Cucumber (Gy14) v1cgycgyB004
Cucsa.126580Cucumber (Gy14) v1cgycgyB023
Cucsa.126580Cucumber (Gy14) v1cgycgyB076
Cucsa.126580Cucurbita maxima (Rimu)cgycmaB0335
Cucsa.126580Cucurbita maxima (Rimu)cgycmaB0336
Cucsa.126580Cucurbita maxima (Rimu)cgycmaB0341
Cucsa.126580Cucurbita moschata (Rifu)cgycmoB0329
Cucsa.126580Cucurbita moschata (Rifu)cgycmoB0330
Cucsa.126580Cucurbita moschata (Rifu)cgycmoB0333
Cucsa.126580Wild cucumber (PI 183967)cgycpiB173
Cucsa.126580Wild cucumber (PI 183967)cgycpiB175
Cucsa.126580Wild cucumber (PI 183967)cgycpiB176
Cucsa.126580Cucumber (Chinese Long) v2cgycuB171
Cucsa.126580Cucumber (Chinese Long) v2cgycuB172
Cucsa.126580Cucumber (Chinese Long) v2cgycuB173
Cucsa.126580Melon (DHL92) v3.5.1cgymeB199
Cucsa.126580Melon (DHL92) v3.5.1cgymeB200
Cucsa.126580Melon (DHL92) v3.5.1cgymeB201
Cucsa.126580Watermelon (Charleston Gray)cgywcgB201
Cucsa.126580Watermelon (97103) v1cgywmB209
Cucsa.126580Cucurbita pepo (Zucchini)cgycpeB0327
Cucsa.126580Cucurbita pepo (Zucchini)cgycpeB0329
Cucsa.126580Cucurbita pepo (Zucchini)cgycpeB0330
Cucsa.126580Melon (DHL92) v3.6.1cgymedB198
Cucsa.126580Silver-seed gourdcarcgyB0716
Cucsa.126580Silver-seed gourdcarcgyB0809
Cucsa.126580Cucumber (Chinese Long) v3cgycucB175
Cucsa.126580Cucumber (Chinese Long) v3cgycucB176
Cucsa.126580Cucumber (Chinese Long) v3cgycucB177
Cucsa.126580Watermelon (97103) v2cgywmbB198
Cucsa.126580Watermelon (97103) v2cgywmbB199