Cucsa.066670 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.066670 vs. Swiss-Prot
Match: ROGF7_ARATH (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.7e-196
Identity = 365/531 (68.74%), Postives = 419/531 (78.91%), Query Frame = 1

Query: 26  IEEHGRESSSSSDFLTSETTGLEEHSHSGS--ED-SSSPSSSSWSTRKAEAPDCIKANGV 85
           +EE GRESS  S    SETT  EE   S S  ED ++SP SS WS +             
Sbjct: 11  VEEKGRESSCCS----SETTRQEEEEQSPSCTEDFTASPVSSRWSVKN------------ 70

Query: 86  VNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC 145
           ++G+K  +      +  S  SE+EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC
Sbjct: 71  IDGEKKKI------RSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLC 130

Query: 146 ATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPRS 205
           ATLFGQLWRLEPLP+EKK MWRREM+WLLCVSDHIVE+TPTWQTFPDG+KLE+MTCRPRS
Sbjct: 131 ATLFGQLWRLEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRS 190

Query: 206 DLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPET--DGSSSLERMLQRQEEKWW 265
           DLYVNLPALRKLDNMLLEILDSFE+ EFWYVDQGI+A E+  DGSSS  +  QRQE+KWW
Sbjct: 191 DLYVNLPALRKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWW 250

Query: 266 LPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRA 325
           LPVPRV PGGLQENSR+QLQHKR+ TNQILKAAMAINS TLAD+EIPE YLE+LP+ GR+
Sbjct: 251 LPVPRVSPGGLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRS 310

Query: 326 CLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPATNTTK 385
           CLGDLIYRYISSD FSPECLLDCL++SSEHQA EIANRVE+SIY+W KRTNSKPATNT  
Sbjct: 311 CLGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNT-- 370

Query: 386 QSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKS 445
              K+SWEMVKELMVD++K EL+A+RAE+LLL LKQRFPGLPQT LDMSKIQYNKD+GKS
Sbjct: 371 ---KTSWEMVKELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKS 430

Query: 446 ILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLGVISHKNSPLHFPVPIS 505
           ILESYSRVLESLAFN+VARIDDLL+VDDLT+HS  Q+   + LG  +  ++P    VP+S
Sbjct: 431 ILESYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIP--TTLG-NNGNDAPKSIAVPVS 490

Query: 506 GTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGFGVKKVLTDYLS 552
                  + TPS+SP++    +     P     KIP     VK+VLT Y++
Sbjct: 491 N------YTTPSYSPSKQELRSSITVPPSPSRFKIPHSS-SVKRVLTAYVT 504

BLAST of Cucsa.066670 vs. Swiss-Prot
Match: ROGF5_ARATH (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 2.9e-177
Identity = 336/586 (57.34%), Postives = 421/586 (71.84%), Query Frame = 1

Query: 25  LIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSSPSSSSWSTRKAEAPDCIKANGVVN 84
           L E   R S+S + + +S TT +     S S          W  RKA      K +  VN
Sbjct: 26  LTESKERSSTSGASYESSSTTTVA----SSSPPPPPSQILGWPIRKASFRKNSKES--VN 85

Query: 85  GDKNHV---DNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNL 144
            D   +   D+   + K  +S+++EMMKERF+KLLLGEDMSG G GVCTALAISNAITNL
Sbjct: 86  FDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNL 145

Query: 145 CATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPR 204
           CAT+FGQLWRLEPL SEKK MWRREM+W+L VSDHIVELTP+ QT+PDG+K EVMTCRPR
Sbjct: 146 CATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPR 205

Query: 205 SDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSLERMLQRQEEKWWL 264
            DL++NLPALRKLDNMLL+IL SF+  EFWYVDQGI+A E DGS+S  R +QRQEEKWWL
Sbjct: 206 FDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQRQEEKWWL 265

Query: 265 PVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRAC 324
           PVPR+ P GL E +R +L HKRE   QILKAAMAINS  L ++++P+ YLE LPKNGR+C
Sbjct: 266 PVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGRSC 325

Query: 325 LGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPA--TNTT 384
           LGD+IYRY++SD FS E LLDCL++SSEH A +IANRVEASIYVWR+R  +K     NT+
Sbjct: 326 LGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNNTS 385

Query: 385 KQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGK 444
             + K +WEMVKELM   +KR LL ER+ETLL CLKQRFP L QT+LD+SKIQ+NKD+GK
Sbjct: 386 STTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNKDIGK 445

Query: 445 SILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD--HQLSSISKLGVISHKN-SPLHFP 504
           SILESYSR LESLA N++ARIDDLLYVDDLTK SD  + LSS +   +I+HK   PL + 
Sbjct: 446 SILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIAHKKVVPLPYL 505

Query: 505 VPISGTPYKTPFA-TPSFSPAQTASPAKGE-RSPF-------IPGTKIPQRGFGVKKVLT 564
           +  SGTPY+T F+ TP FSP+   SP KGE R+P+       I    +P RG+GV++VL 
Sbjct: 506 ISASGTPYRTSFSTTPGFSPSM-ISPKKGERRTPYSSKDTNKIIEKGLPSRGYGVRRVLN 565

Query: 565 DYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQR 594
           +YL +E++  K   N  + A+    +++ +   + +   ++S  Q+
Sbjct: 566 NYLGMESK-LKICVNPSDNADTAVINQISKDVEEEKKRNSTSVHQK 603

BLAST of Cucsa.066670 vs. Swiss-Prot
Match: ROFG6_ARATH (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.4e-144
Identity = 293/555 (52.79%), Postives = 377/555 (67.93%), Query Frame = 1

Query: 38  DFLTSETTGLEEHSHSGSEDSSSPSSSSWSTRKAEAPDCIKANGVVNGDKN-HVDNRKLE 97
           D +T  TT     S S    SSS  SS      +  P   +  G   G  +    + K++
Sbjct: 27  DSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMK 86

Query: 98  KKV-------------SSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCA 157
           KK              + +SEIE++KER +KLLLGEDMSG G GVC ALAISNAITNL A
Sbjct: 87  KKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYA 146

Query: 158 TLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPRSD 217
            + GQ WRLEP+PSEKKLMWRRE++ LL VSDHIVEL P++Q FP+G+K+EVM CRPRSD
Sbjct: 147 AILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSD 206

Query: 218 LYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSLERMLQRQEEKWWLPV 277
           L+  LPALRKLDNML+EILDSF + EFWYVDQGI+A E+  S+S     +   +KWWLP+
Sbjct: 207 LFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESARSNS----FREDGDKWWLPL 266

Query: 278 PRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRACLG 337
           PRVP  GL E +R++L H RE TNQILKA M+INS  LA++E+P+ YLEALPKNGR+CLG
Sbjct: 267 PRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLG 326

Query: 338 DLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPATNTTKQSS 397
           D +YR I+SD+FS + LL+ +++SSE    E+ANRVEAS+YVWR+R +S+   +  + +S
Sbjct: 327 DFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTS 386

Query: 398 KSSWEMVKELMV---DSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKS 457
                +VKE+M+   D +KRE+ AERAE+LL+ LKQRFPGL QT LD SKIQYNKDVGKS
Sbjct: 387 TRWGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKS 446

Query: 458 ILESYSRVLESLAFNVVARIDDLLYVDDLTK-HSDHQLSSIS-KLGVISHK--------- 517
           ILESYSRVLESLA+++  RI+++L++DD++K   D    S S KL ++S +         
Sbjct: 447 ILESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSL 506

Query: 518 ----NSPLHFPVPISG--TPYKT---PFATPSFSPAQTASPAK----GERSPFIPGTKIP 551
               ++P  F V  SG  TPY+T     +TPS+SP    SP      GER+PF+ G  I 
Sbjct: 507 REKLSAPSLFSVSFSGTSTPYRTLSFSASTPSYSPMPLISPINGGRGGERAPFLSGRNIR 566

BLAST of Cucsa.066670 vs. Swiss-Prot
Match: ROGF1_ARATH (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 508.4 bits (1308), Expect = 1.0e-142
Identity = 276/508 (54.33%), Postives = 353/508 (69.49%), Query Frame = 1

Query: 30  GRESSSSSDFLTSETTGLEEHSHSGSED-SSSPSSSSWSTRKAE----------APDCIK 89
           G  SS   D ++SE  G    S+S S D S S SSSS+S  + +          +P  + 
Sbjct: 2   GSLSSEEDDEVSSERCG----SYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVA 61

Query: 90  ANG----------VVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGV 149
             G          V+ G     D+++ +  +S   EIEMMKERF+KLLLGEDMSG G GV
Sbjct: 62  GRGFYFPAPVMLPVIGGKDVVWDDKQPDNDLS---EIEMMKERFAKLLLGEDMSGGGKGV 121

Query: 150 CTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFP 209
           CTALAISNAITNL AT+FG+LWRLEPL  +KK MWRRE++WLLCVSD IVEL P+ Q FP
Sbjct: 122 CTALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFP 181

Query: 210 DGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSL 269
            G   E+M  RPRSDLY NLPAL+KLD ML+++LD+F D EFWY D+GI+  + D  S  
Sbjct: 182 GGGTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYN 241

Query: 270 ERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPE 329
                RQE+KWWLP P+VPP GL E +R++LQ  R+  NQILKAA+AINS  LA++EIP+
Sbjct: 242 SPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPD 301

Query: 330 PYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRK 389
           PYLE LPK+G+ CLG++IY+Y++++ FSPECLLDCL++SSEHQ  EIANR+EA+++VWR+
Sbjct: 302 PYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQ 361

Query: 390 RTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDM 449
           + N +      K    S    VK L+ D+E+ + L +RAETLL  L+ RFPGLPQTTLDM
Sbjct: 362 K-NGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDM 421

Query: 450 SKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLGVISH 509
           +KIQYNKDVG+SILESYSRV+ES+AFN+ ARIDD+LYVDD  + S     S+S L  I+ 
Sbjct: 422 NKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLS-LFSING 481

Query: 510 KNSPLHFPVPISGTPYKTPFATPSFSPA 517
            N    F V    +P+ +PFATP+ S A
Sbjct: 482 LNPQKAFSV--QSSPHGSPFATPALSVA 498

BLAST of Cucsa.066670 vs. Swiss-Prot
Match: ROGF3_ARATH (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.5e-122
Identity = 226/381 (59.32%), Postives = 295/381 (77.43%), Query Frame = 1

Query: 101 SSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKK 160
           S  E+E MKERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEPL +EK+
Sbjct: 101 SEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKR 160

Query: 161 LMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLE 220
            +W+REM+ LL V D+IVE  P  Q   +G+ +EVM  RPR+D+Y+NLPALRKLD+ML+E
Sbjct: 161 ALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLME 220

Query: 221 ILDSFEDCEFWYVDQGILAPETDGSSS---LERMLQRQEEKWWLPVPRVPPGGLQENSRR 280
            LDSF++ EFWY ++G L+ ++  SS+    + ++QR+EEKWWLPVP VP  GL + +R+
Sbjct: 221 ALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARK 280

Query: 281 QLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRACLGDLIYRYIS-SDHFS 340
           QL++KRESTNQI KAAMAINS+ L+++EIP+ Y+  LPK G++ +GD IYRY+S S  F 
Sbjct: 281 QLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFF 340

Query: 341 PECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVD 400
           PE LLDCL +SSEH+A ++A+RVEAS+Y WR++        +   +SK+SW MVK+LM  
Sbjct: 341 PEQLLDCLNISSEHEAVQLADRVEASMYTWRRK--------SCLSNSKNSWNMVKDLMST 400

Query: 401 SE---KRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKSILESYSRVLESLA 460
           +E   K  ++AERAETLL CLKQR+P L QT+LD+ KIQYNKDVGK++LESYSRVLE LA
Sbjct: 401 TERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLA 460

Query: 461 FNVVARIDDLLYVDDLTKHSD 475
           FN+VA IDD+LYVD   + S+
Sbjct: 461 FNIVAWIDDVLYVDKTMRGSE 473

BLAST of Cucsa.066670 vs. TrEMBL
Match: A0A0A0KXQ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G303120 PE=4 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 597/598 (99.83%), Postives = 597/598 (99.83%), Query Frame = 1

Query: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60
           MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSS 
Sbjct: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSP 60

Query: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120
           PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300
           ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360
           LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420
           ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540
           SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 599
           GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 598

BLAST of Cucsa.066670 vs. TrEMBL
Match: M5VXJ9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002319mg PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 9.4e-252
Identity = 440/581 (75.73%), Postives = 495/581 (85.20%), Query Frame = 1

Query: 3   ESLIE-KSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS-EDSSS 62
           E +IE K +  G  + +  T G LIEE GR+SSSSS+FLTSETTG EE SHS S E++SS
Sbjct: 85  EGIIEGKREDSGECLGQVGTFGDLIEEKGRDSSSSSEFLTSETTGHEEQSHSSSDEETSS 144

Query: 63  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 122
             S  W  +KAEA DC   NG    ++ H+D+R LEK+ S+ SE E+MKERFSKLLLGED
Sbjct: 145 APSLGWPIQKAEASDCTSQNGSEAAERTHLDDRNLEKQGSTISETELMKERFSKLLLGED 204

Query: 123 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 182
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKKLMWRREM+WLLCVSDHIVEL
Sbjct: 205 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPQEKKLMWRREMEWLLCVSDHIVEL 264

Query: 183 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 242
            P+WQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSF + EFWYVDQGILAP
Sbjct: 265 IPSWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFVNTEFWYVDQGILAP 324

Query: 243 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 302
           + DGSSS  R  QRQEEKWWLPVPRVPPGGL EN++RQLQHKR+ T+QILKAAMAINS T
Sbjct: 325 DADGSSSFRRAFQRQEEKWWLPVPRVPPGGLHENTKRQLQHKRDCTSQILKAAMAINSIT 384

Query: 303 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 362
           LAD+E+PE YLEALPKNGRA LGDLIYRYISSD FSPECLLDCL++SSEHQA EIANRVE
Sbjct: 385 LADMEVPESYLEALPKNGRASLGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVE 444

Query: 363 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 422
           ASIY+WRK+ NSKP  N T+ SS+SSWEMVKELMVD+EK+ELL ERAE+LL+CL+QRFPG
Sbjct: 445 ASIYLWRKKANSKPTNNATRFSSRSSWEMVKELMVDAEKKELLPERAESLLMCLRQRFPG 504

Query: 423 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 482
           LPQT LDMSKIQYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLT+HSD Q SSI
Sbjct: 505 LPQTALDMSKIQYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTRHSD-QFSSI 564

Query: 483 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 542
           SK+ +ISHK++P  + VPIS TPYKT F TPSFSP Q  SP +G+RSPFI   KIPQRG 
Sbjct: 565 SKVSIISHKSTPNPYSVPISSTPYKTAFTTPSFSPGQLVSPLRGDRSPFITSGKIPQRGL 624

Query: 543 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQ 582
           GVKKVLTDYLS++A+ GK+ S+ IEK + ++N+  E   SQ
Sbjct: 625 GVKKVLTDYLSIDAK-GKDYSDPIEKPDSVSNTMQETLGSQ 663

BLAST of Cucsa.066670 vs. TrEMBL
Match: B9HC61_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s09370g PE=4 SV=2)

HSP 1 Score: 863.2 bits (2229), Expect = 1.8e-247
Identity = 440/594 (74.07%), Postives = 496/594 (83.50%), Query Frame = 1

Query: 7   EKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSED-SSSPSSSS 66
           EK    G    E +T G LIE+  RESSSSSDFLTSE TG EEH HS SE+ SSSP +  
Sbjct: 14  EKDTNFGEKKGEVQTFGDLIEDKCRESSSSSDFLTSEATGNEEHGHSSSEEGSSSPHTMG 73

Query: 67  WSTRKAEAPDCIKANGVVNGD-KNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGC 126
           W   K EAPDC   +G  + + K+H D+RKL K+ SS SE EMMKERFSKLLLGEDMSGC
Sbjct: 74  WPVLKDEAPDCTNTDGATDDEEKSHFDDRKLGKQGSSVSETEMMKERFSKLLLGEDMSGC 133

Query: 127 GNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTW 186
           GNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK MWRREM+WLLCVSDHIVEL P+W
Sbjct: 134 GNGVCTALAISNAITNLCATLFGQLWRLEPLPLEKKAMWRREMEWLLCVSDHIVELMPSW 193

Query: 187 QTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDG 246
           QTFPDGSKLEVMTC PRSDLY+NLPALRKLDNMLLEILDSF++ EFWYVDQGILAP+TDG
Sbjct: 194 QTFPDGSKLEVMTCGPRSDLYINLPALRKLDNMLLEILDSFDNTEFWYVDQGILAPDTDG 253

Query: 247 SSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADL 306
           S+S  R LQRQEEKWWLPVP+VPPGGL ENSR++LQHKR+STNQILKAAMAINS TLA++
Sbjct: 254 SASFRRTLQRQEEKWWLPVPQVPPGGLHENSRKKLQHKRDSTNQILKAAMAINSITLAEM 313

Query: 307 EIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIY 366
           EIPE YLEALPKNG+A LGDLIYRYISSD F PECLLDCL++SSEHQA E+ANRVEASIY
Sbjct: 314 EIPESYLEALPKNGKASLGDLIYRYISSDQFYPECLLDCLDLSSEHQAIELANRVEASIY 373

Query: 367 VWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQT 426
           +WRKRTN KPA++T + SSKSSWE+VKELM+D++KRELLA+RAE+LLLCLKQRFPGLPQT
Sbjct: 374 IWRKRTNYKPASSTNRSSSKSSWELVKELMIDADKRELLADRAESLLLCLKQRFPGLPQT 433

Query: 427 TLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLG 486
           TLDMSKIQYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLTKHSDH  SSISK+ 
Sbjct: 434 TLDMSKIQYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTKHSDH-FSSISKVS 493

Query: 487 VISHKNSPLHFPVPISGTPYKTPFATPSFSPA-QTASPAKGERSPFIPGTKIPQRGFGVK 546
           VI+HK+  + + VP+S TPYKT F TPSFSP  Q  SPAKG+RSPF+   KIPQRG GVK
Sbjct: 494 VIAHKSVTIPYSVPVSNTPYKTAFNTPSFSPGQQRISPAKGDRSPFMTSGKIPQRGLGVK 553

Query: 547 KVLTDYLSVEARGGKELSNKIEKAE-PITNSKLERQASQYRGGV---ASSPRQR 594
           KVLT+YLS++   G++  N IE A+  I N+   +   +  G +    SSP  R
Sbjct: 554 KVLTEYLSIDTE-GRDGGNMIEGADNVIRNTSASQTGVESFGSIIETISSPENR 605

BLAST of Cucsa.066670 vs. TrEMBL
Match: A0A061ESK3_THECC (RHO guanyl-nucleotide exchange factor 7 OS=Theobroma cacao GN=TCM_021824 PE=4 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 2.9e-245
Identity = 431/569 (75.75%), Postives = 486/569 (85.41%), Query Frame = 1

Query: 3   ESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEE-HSHSGSED-SSS 62
           E   +K+++     ++  T   L+E+ GRESSSSSDFLTSETTG EE HSHS SE+ S+S
Sbjct: 94  EGFEDKNESFNENGVQMGTFAKLLEDKGRESSSSSDFLTSETTGHEEEHSHSSSEEESAS 153

Query: 63  PSSSSWSTRK-AEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 122
           P S  W  +K AE  DC  +N   +G K   D+RKLEK+ S+ SEIE+MKERF+KLLLGE
Sbjct: 154 PPSLGWPVQKNAETEDCSSSNCSEDGKKPPSDDRKLEKQGSTVSEIELMKERFAKLLLGE 213

Query: 123 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVE 182
           DMSGCGNGVCTALAISNAITNLCATLFGQ+WRLEPLP EKK MWRREM+WLL VSDHIVE
Sbjct: 214 DMSGCGNGVCTALAISNAITNLCATLFGQIWRLEPLPVEKKAMWRREMEWLLSVSDHIVE 273

Query: 183 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 242
           L P+WQTFPDGSKLEVMTCRPRSDLY+NLPALRKLDNMLLEILDSF D EFWYVDQGILA
Sbjct: 274 LIPSWQTFPDGSKLEVMTCRPRSDLYINLPALRKLDNMLLEILDSFVDTEFWYVDQGILA 333

Query: 243 PETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINST 302
           PE DGS+S  + LQRQEEKWWLPVPRVPPGGL ENSR+QLQHKR+ TNQILKAAMAINS 
Sbjct: 334 PEADGSTSFRKALQRQEEKWWLPVPRVPPGGLHENSRKQLQHKRDCTNQILKAAMAINSI 393

Query: 303 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRV 362
           TLAD+E+PE YLE+LPKNGRA LGDLIYRYISSD F+PECLLDCL++SSEHQA EIANRV
Sbjct: 394 TLADMEVPESYLESLPKNGRASLGDLIYRYISSDLFTPECLLDCLDLSSEHQAIEIANRV 453

Query: 363 EASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 422
           EASIY+WRK+TNSKP  NTT+ SSKSSWEMVK+LMVD+EKRE+LA+RAE+LLLCLKQRFP
Sbjct: 454 EASIYLWRKKTNSKPVNNTTRSSSKSSWEMVKDLMVDAEKREILADRAESLLLCLKQRFP 513

Query: 423 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSS 482
           GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLTKHSD Q SS
Sbjct: 514 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTKHSD-QFSS 573

Query: 483 ISKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRG 542
           ++++GVI  K+  + + VP S TPY+T F TP+FSPAQ  SPAKGERSP++  +K  QRG
Sbjct: 574 LTRVGVIGQKSLSIPYSVPFSSTPYRTAFTTPNFSPAQLVSPAKGERSPYLTSSKFQQRG 633

Query: 543 FGVKKVLTDYLSVEARGGKELSNKIEKAE 569
            GVKKVLTDYLS++ R GKE SN  E  E
Sbjct: 634 IGVKKVLTDYLSIDVR-GKECSNSNEGTE 660

BLAST of Cucsa.066670 vs. TrEMBL
Match: A0A067JKL9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25095 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 7.5e-241
Identity = 420/555 (75.68%), Postives = 478/555 (86.13%), Query Frame = 1

Query: 16  VLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSE--DSSSPSSSSWSTRKAE- 75
           + E  T G LIEE GRESSSSSDFLTSETTGLEE SHS SE  DSSSP S  W  +K E 
Sbjct: 118 ISEMHTFGDLIEEKGRESSSSSDFLTSETTGLEEQSHSSSEEEDSSSPPSMGWPVKKEEE 177

Query: 76  APDCIKANGVVN-GDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTA 135
            PDC   NG  +  +K ++D++KLEK+ SS SEIEMMKERFSKLLLGEDMSGCGNGVCTA
Sbjct: 178 VPDCTSTNGATDEAEKINLDDKKLEKQRSSISEIEMMKERFSKLLLGEDMSGCGNGVCTA 237

Query: 136 LAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGS 195
           LAISNAITNLCAT+FGQLWRLEPLP EKK MWRREM+WLLCVSDHIVELTP+WQTFPDGS
Sbjct: 238 LAISNAITNLCATMFGQLWRLEPLPHEKKAMWRREMEWLLCVSDHIVELTPSWQTFPDGS 297

Query: 196 KLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSLERM 255
           KLEVMT RPRSDLY+NLPALRKLDNMLLEILDSF++ EFWYVDQGILAP+ DGSSS  R 
Sbjct: 298 KLEVMTSRPRSDLYINLPALRKLDNMLLEILDSFDNTEFWYVDQGILAPDADGSSSFRRT 357

Query: 256 LQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYL 315
            QRQEEKWWLPVPRVPP GL ENSR+QLQHKR+ TNQILKAAMAINS  LAD+++PE +L
Sbjct: 358 FQRQEEKWWLPVPRVPPCGLHENSRKQLQHKRDCTNQILKAAMAINSIALADMQVPESFL 417

Query: 316 EALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTN 375
           +ALPKN +A LGDLIYRYISSD FSPECLLDCL++SSEHQA E+ANRVEASIYVWR+RTN
Sbjct: 418 DALPKNAKASLGDLIYRYISSDQFSPECLLDCLDLSSEHQAIELANRVEASIYVWRRRTN 477

Query: 376 SKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKI 435
           S+P  NT + SSKSSWE+VKELM+D+EKRELLA+RAE+LLLCL+QR+PGLPQTTLDMSKI
Sbjct: 478 SRPTNNTNRSSSKSSWELVKELMIDAEKRELLADRAESLLLCLRQRYPGLPQTTLDMSKI 537

Query: 436 QYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLGVISHKNS 495
           QYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLTKHSD Q SSISK+ +I+HK+ 
Sbjct: 538 QYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTKHSD-QFSSISKVSLIAHKSV 597

Query: 496 PLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF-GVKKVLTDYL 555
            + + VP+  TPYKT + TPS+SP+  ASPAKG+ SP +  ++I Q+   G+KKVLTDYL
Sbjct: 598 SIPYSVPVPSTPYKTAYTTPSYSPSHLASPAKGDISPLMTSSRIQQQHRPGIKKVLTDYL 657

Query: 556 SVEARGGKELSNKIE 566
           S++ + GK+ SN  E
Sbjct: 658 SIDTK-GKDFSNATE 670

BLAST of Cucsa.066670 vs. TAIR10
Match: AT5G02010.1 (AT5G02010.1 RHO guanyl-nucleotide exchange factor 7)

HSP 1 Score: 685.6 bits (1768), Expect = 2.7e-197
Identity = 365/531 (68.74%), Postives = 419/531 (78.91%), Query Frame = 1

Query: 26  IEEHGRESSSSSDFLTSETTGLEEHSHSGS--ED-SSSPSSSSWSTRKAEAPDCIKANGV 85
           +EE GRESS  S    SETT  EE   S S  ED ++SP SS WS +             
Sbjct: 11  VEEKGRESSCCS----SETTRQEEEEQSPSCTEDFTASPVSSRWSVKN------------ 70

Query: 86  VNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC 145
           ++G+K  +      +  S  SE+EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC
Sbjct: 71  IDGEKKKI------RSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLC 130

Query: 146 ATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPRS 205
           ATLFGQLWRLEPLP+EKK MWRREM+WLLCVSDHIVE+TPTWQTFPDG+KLE+MTCRPRS
Sbjct: 131 ATLFGQLWRLEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRS 190

Query: 206 DLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPET--DGSSSLERMLQRQEEKWW 265
           DLYVNLPALRKLDNMLLEILDSFE+ EFWYVDQGI+A E+  DGSSS  +  QRQE+KWW
Sbjct: 191 DLYVNLPALRKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWW 250

Query: 266 LPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRA 325
           LPVPRV PGGLQENSR+QLQHKR+ TNQILKAAMAINS TLAD+EIPE YLE+LP+ GR+
Sbjct: 251 LPVPRVSPGGLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRS 310

Query: 326 CLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPATNTTK 385
           CLGDLIYRYISSD FSPECLLDCL++SSEHQA EIANRVE+SIY+W KRTNSKPATNT  
Sbjct: 311 CLGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNT-- 370

Query: 386 QSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKS 445
              K+SWEMVKELMVD++K EL+A+RAE+LLL LKQRFPGLPQT LDMSKIQYNKD+GKS
Sbjct: 371 ---KTSWEMVKELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKS 430

Query: 446 ILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLGVISHKNSPLHFPVPIS 505
           ILESYSRVLESLAFN+VARIDDLL+VDDLT+HS  Q+   + LG  +  ++P    VP+S
Sbjct: 431 ILESYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIP--TTLG-NNGNDAPKSIAVPVS 490

Query: 506 GTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGFGVKKVLTDYLS 552
                  + TPS+SP++    +     P     KIP     VK+VLT Y++
Sbjct: 491 N------YTTPSYSPSKQELRSSITVPPSPSRFKIPHSS-SVKRVLTAYVT 504

BLAST of Cucsa.066670 vs. TAIR10
Match: AT5G05940.1 (AT5G05940.1 ROP guanine nucleotide exchange factor 5)

HSP 1 Score: 623.2 bits (1606), Expect = 1.6e-178
Identity = 336/586 (57.34%), Postives = 421/586 (71.84%), Query Frame = 1

Query: 25  LIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSSPSSSSWSTRKAEAPDCIKANGVVN 84
           L E   R S+S + + +S TT +     S S          W  RKA      K +  VN
Sbjct: 26  LTESKERSSTSGASYESSSTTTVA----SSSPPPPPSQILGWPIRKASFRKNSKES--VN 85

Query: 85  GDKNHV---DNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNL 144
            D   +   D+   + K  +S+++EMMKERF+KLLLGEDMSG G GVCTALAISNAITNL
Sbjct: 86  FDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNL 145

Query: 145 CATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPR 204
           CAT+FGQLWRLEPL SEKK MWRREM+W+L VSDHIVELTP+ QT+PDG+K EVMTCRPR
Sbjct: 146 CATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPR 205

Query: 205 SDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSLERMLQRQEEKWWL 264
            DL++NLPALRKLDNMLL+IL SF+  EFWYVDQGI+A E DGS+S  R +QRQEEKWWL
Sbjct: 206 FDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQRQEEKWWL 265

Query: 265 PVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRAC 324
           PVPR+ P GL E +R +L HKRE   QILKAAMAINS  L ++++P+ YLE LPKNGR+C
Sbjct: 266 PVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGRSC 325

Query: 325 LGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPA--TNTT 384
           LGD+IYRY++SD FS E LLDCL++SSEH A +IANRVEASIYVWR+R  +K     NT+
Sbjct: 326 LGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNNTS 385

Query: 385 KQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGK 444
             + K +WEMVKELM   +KR LL ER+ETLL CLKQRFP L QT+LD+SKIQ+NKD+GK
Sbjct: 386 STTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNKDIGK 445

Query: 445 SILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD--HQLSSISKLGVISHKN-SPLHFP 504
           SILESYSR LESLA N++ARIDDLLYVDDLTK SD  + LSS +   +I+HK   PL + 
Sbjct: 446 SILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIAHKKVVPLPYL 505

Query: 505 VPISGTPYKTPFA-TPSFSPAQTASPAKGE-RSPF-------IPGTKIPQRGFGVKKVLT 564
           +  SGTPY+T F+ TP FSP+   SP KGE R+P+       I    +P RG+GV++VL 
Sbjct: 506 ISASGTPYRTSFSTTPGFSPSM-ISPKKGERRTPYSSKDTNKIIEKGLPSRGYGVRRVLN 565

Query: 565 DYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQR 594
           +YL +E++  K   N  + A+    +++ +   + +   ++S  Q+
Sbjct: 566 NYLGMESK-LKICVNPSDNADTAVINQISKDVEEEKKRNSTSVHQK 603

BLAST of Cucsa.066670 vs. TAIR10
Match: AT3G55660.1 (AT3G55660.1 ROP (rho of plants) guanine nucleotide exchange factor 6)

HSP 1 Score: 514.6 bits (1324), Expect = 8.1e-146
Identity = 293/555 (52.79%), Postives = 377/555 (67.93%), Query Frame = 1

Query: 38  DFLTSETTGLEEHSHSGSEDSSSPSSSSWSTRKAEAPDCIKANGVVNGDKN-HVDNRKLE 97
           D +T  TT     S S    SSS  SS      +  P   +  G   G  +    + K++
Sbjct: 27  DSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMK 86

Query: 98  KKV-------------SSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCA 157
           KK              + +SEIE++KER +KLLLGEDMSG G GVC ALAISNAITNL A
Sbjct: 87  KKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYA 146

Query: 158 TLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPRSD 217
            + GQ WRLEP+PSEKKLMWRRE++ LL VSDHIVEL P++Q FP+G+K+EVM CRPRSD
Sbjct: 147 AILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSD 206

Query: 218 LYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSLERMLQRQEEKWWLPV 277
           L+  LPALRKLDNML+EILDSF + EFWYVDQGI+A E+  S+S     +   +KWWLP+
Sbjct: 207 LFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESARSNS----FREDGDKWWLPL 266

Query: 278 PRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRACLG 337
           PRVP  GL E +R++L H RE TNQILKA M+INS  LA++E+P+ YLEALPKNGR+CLG
Sbjct: 267 PRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLG 326

Query: 338 DLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPATNTTKQSS 397
           D +YR I+SD+FS + LL+ +++SSE    E+ANRVEAS+YVWR+R +S+   +  + +S
Sbjct: 327 DFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTS 386

Query: 398 KSSWEMVKELMV---DSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKS 457
                +VKE+M+   D +KRE+ AERAE+LL+ LKQRFPGL QT LD SKIQYNKDVGKS
Sbjct: 387 TRWGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKS 446

Query: 458 ILESYSRVLESLAFNVVARIDDLLYVDDLTK-HSDHQLSSIS-KLGVISHK--------- 517
           ILESYSRVLESLA+++  RI+++L++DD++K   D    S S KL ++S +         
Sbjct: 447 ILESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSL 506

Query: 518 ----NSPLHFPVPISG--TPYKT---PFATPSFSPAQTASPAK----GERSPFIPGTKIP 551
               ++P  F V  SG  TPY+T     +TPS+SP    SP      GER+PF+ G  I 
Sbjct: 507 REKLSAPSLFSVSFSGTSTPYRTLSFSASTPSYSPMPLISPINGGRGGERAPFLSGRNIR 566

BLAST of Cucsa.066670 vs. TAIR10
Match: AT4G38430.1 (AT4G38430.1 rho guanyl-nucleotide exchange factor 1)

HSP 1 Score: 508.4 bits (1308), Expect = 5.8e-144
Identity = 276/508 (54.33%), Postives = 353/508 (69.49%), Query Frame = 1

Query: 30  GRESSSSSDFLTSETTGLEEHSHSGSED-SSSPSSSSWSTRKAE----------APDCIK 89
           G  SS   D ++SE  G    S+S S D S S SSSS+S  + +          +P  + 
Sbjct: 2   GSLSSEEDDEVSSERCG----SYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVA 61

Query: 90  ANG----------VVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGV 149
             G          V+ G     D+++ +  +S   EIEMMKERF+KLLLGEDMSG G GV
Sbjct: 62  GRGFYFPAPVMLPVIGGKDVVWDDKQPDNDLS---EIEMMKERFAKLLLGEDMSGGGKGV 121

Query: 150 CTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTWQTFP 209
           CTALAISNAITNL AT+FG+LWRLEPL  +KK MWRRE++WLLCVSD IVEL P+ Q FP
Sbjct: 122 CTALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFP 181

Query: 210 DGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDGSSSL 269
            G   E+M  RPRSDLY NLPAL+KLD ML+++LD+F D EFWY D+GI+  + D  S  
Sbjct: 182 GGGTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYN 241

Query: 270 ERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADLEIPE 329
                RQE+KWWLP P+VPP GL E +R++LQ  R+  NQILKAA+AINS  LA++EIP+
Sbjct: 242 SPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPD 301

Query: 330 PYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIYVWRK 389
           PYLE LPK+G+ CLG++IY+Y++++ FSPECLLDCL++SSEHQ  EIANR+EA+++VWR+
Sbjct: 302 PYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQ 361

Query: 390 RTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDM 449
           + N +      K    S    VK L+ D+E+ + L +RAETLL  L+ RFPGLPQTTLDM
Sbjct: 362 K-NGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDM 421

Query: 450 SKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLGVISH 509
           +KIQYNKDVG+SILESYSRV+ES+AFN+ ARIDD+LYVDD  + S     S+S L  I+ 
Sbjct: 422 NKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLS-LFSING 481

Query: 510 KNSPLHFPVPISGTPYKTPFATPSFSPA 517
            N    F V    +P+ +PFATP+ S A
Sbjct: 482 LNPQKAFSV--QSSPHGSPFATPALSVA 498

BLAST of Cucsa.066670 vs. TAIR10
Match: AT4G00460.2 (AT4G00460.2 RHO guanyl-nucleotide exchange factor 3)

HSP 1 Score: 441.4 bits (1134), Expect = 8.7e-124
Identity = 226/381 (59.32%), Postives = 295/381 (77.43%), Query Frame = 1

Query: 101 SSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKK 160
           S  E+E MKERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEPL +EK+
Sbjct: 101 SEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKR 160

Query: 161 LMWRREMDWLLCVSDHIVELTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLE 220
            +W+REM+ LL V D+IVE  P  Q   +G+ +EVM  RPR+D+Y+NLPALRKLD+ML+E
Sbjct: 161 ALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLME 220

Query: 221 ILDSFEDCEFWYVDQGILAPETDGSSS---LERMLQRQEEKWWLPVPRVPPGGLQENSRR 280
            LDSF++ EFWY ++G L+ ++  SS+    + ++QR+EEKWWLPVP VP  GL + +R+
Sbjct: 221 ALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARK 280

Query: 281 QLQHKRESTNQILKAAMAINSTTLADLEIPEPYLEALPKNGRACLGDLIYRYIS-SDHFS 340
           QL++KRESTNQI KAAMAINS+ L+++EIP+ Y+  LPK G++ +GD IYRY+S S  F 
Sbjct: 281 QLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFF 340

Query: 341 PECLLDCLEMSSEHQATEIANRVEASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVD 400
           PE LLDCL +SSEH+A ++A+RVEAS+Y WR++        +   +SK+SW MVK+LM  
Sbjct: 341 PEQLLDCLNISSEHEAVQLADRVEASMYTWRRK--------SCLSNSKNSWNMVKDLMST 400

Query: 401 SE---KRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKSILESYSRVLESLA 460
           +E   K  ++AERAETLL CLKQR+P L QT+LD+ KIQYNKDVGK++LESYSRVLE LA
Sbjct: 401 TERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLA 460

Query: 461 FNVVARIDDLLYVDDLTKHSD 475
           FN+VA IDD+LYVD   + S+
Sbjct: 461 FNIVAWIDDVLYVDKTMRGSE 473

BLAST of Cucsa.066670 vs. NCBI nr
Match: gi|449460361|ref|XP_004147914.1| (PREDICTED: rop guanine nucleotide exchange factor 7 [Cucumis sativus])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 597/598 (99.83%), Postives = 597/598 (99.83%), Query Frame = 1

Query: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60
           MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSS 
Sbjct: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSP 60

Query: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120
           PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300
           ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360
           LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420
           ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540
           SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 599
           GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 598

BLAST of Cucsa.066670 vs. NCBI nr
Match: gi|659069194|ref|XP_008448594.1| (PREDICTED: rop guanine nucleotide exchange factor 7-like [Cucumis melo])

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 592/598 (99.00%), Postives = 594/598 (99.33%), Query Frame = 1

Query: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60
           M ESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS
Sbjct: 1   MAESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60

Query: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120
           PSSSSWSTRKAEAPDCIKANGVVNG KNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300
           ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQE+SRRQLQHKRESTNQILKAAMAINSTT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQEDSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360
           LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420
           ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540
           SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 599
           GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQ SQYRGGVASSP+QRSVV E
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQTSQYRGGVASSPKQRSVVGE 598

BLAST of Cucsa.066670 vs. NCBI nr
Match: gi|595822881|ref|XP_007204968.1| (hypothetical protein PRUPE_ppa002319mg [Prunus persica])

HSP 1 Score: 877.5 bits (2266), Expect = 1.4e-251
Identity = 440/581 (75.73%), Postives = 495/581 (85.20%), Query Frame = 1

Query: 3   ESLIE-KSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS-EDSSS 62
           E +IE K +  G  + +  T G LIEE GR+SSSSS+FLTSETTG EE SHS S E++SS
Sbjct: 85  EGIIEGKREDSGECLGQVGTFGDLIEEKGRDSSSSSEFLTSETTGHEEQSHSSSDEETSS 144

Query: 63  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 122
             S  W  +KAEA DC   NG    ++ H+D+R LEK+ S+ SE E+MKERFSKLLLGED
Sbjct: 145 APSLGWPIQKAEASDCTSQNGSEAAERTHLDDRNLEKQGSTISETELMKERFSKLLLGED 204

Query: 123 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 182
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKKLMWRREM+WLLCVSDHIVEL
Sbjct: 205 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPQEKKLMWRREMEWLLCVSDHIVEL 264

Query: 183 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 242
            P+WQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSF + EFWYVDQGILAP
Sbjct: 265 IPSWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFVNTEFWYVDQGILAP 324

Query: 243 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 302
           + DGSSS  R  QRQEEKWWLPVPRVPPGGL EN++RQLQHKR+ T+QILKAAMAINS T
Sbjct: 325 DADGSSSFRRAFQRQEEKWWLPVPRVPPGGLHENTKRQLQHKRDCTSQILKAAMAINSIT 384

Query: 303 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 362
           LAD+E+PE YLEALPKNGRA LGDLIYRYISSD FSPECLLDCL++SSEHQA EIANRVE
Sbjct: 385 LADMEVPESYLEALPKNGRASLGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVE 444

Query: 363 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 422
           ASIY+WRK+ NSKP  N T+ SS+SSWEMVKELMVD+EK+ELL ERAE+LL+CL+QRFPG
Sbjct: 445 ASIYLWRKKANSKPTNNATRFSSRSSWEMVKELMVDAEKKELLPERAESLLMCLRQRFPG 504

Query: 423 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 482
           LPQT LDMSKIQYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLT+HSD Q SSI
Sbjct: 505 LPQTALDMSKIQYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTRHSD-QFSSI 564

Query: 483 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 542
           SK+ +ISHK++P  + VPIS TPYKT F TPSFSP Q  SP +G+RSPFI   KIPQRG 
Sbjct: 565 SKVSIISHKSTPNPYSVPISSTPYKTAFTTPSFSPGQLVSPLRGDRSPFITSGKIPQRGL 624

Query: 543 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQ 582
           GVKKVLTDYLS++A+ GK+ S+ IEK + ++N+  E   SQ
Sbjct: 625 GVKKVLTDYLSIDAK-GKDYSDPIEKPDSVSNTMQETLGSQ 663

BLAST of Cucsa.066670 vs. NCBI nr
Match: gi|645220407|ref|XP_008240123.1| (PREDICTED: rop guanine nucleotide exchange factor 7 [Prunus mume])

HSP 1 Score: 873.6 bits (2256), Expect = 2.0e-250
Identity = 437/581 (75.22%), Postives = 493/581 (84.85%), Query Frame = 1

Query: 3   ESLIE-KSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS-EDSSS 62
           E +IE K +  G  + +  T G LIEE GR SSSSS+FLTSETTG EEHSHS S E++SS
Sbjct: 103 EGIIEGKREDFGECLGQVGTFGDLIEEKGRVSSSSSEFLTSETTGHEEHSHSSSDEETSS 162

Query: 63  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 122
             S  W  +KAEA DC   NG    ++ H+D+R LEK+ S+ SE E+MKERFSKLLLGED
Sbjct: 163 APSLGWPIQKAEASDCTSQNGSEAAERTHLDDRNLEKQGSTISETELMKERFSKLLLGED 222

Query: 123 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 182
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKKLMWRREM+WLLCVSDHIVEL
Sbjct: 223 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPQEKKLMWRREMEWLLCVSDHIVEL 282

Query: 183 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 242
            P+WQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSF + EFWYVDQGILAP
Sbjct: 283 IPSWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFVNTEFWYVDQGILAP 342

Query: 243 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 302
           + DGSSS  R  QRQEEKWWLPVPRVPPGGL EN+++QLQHKR+ T+QILKAAMAIN+ T
Sbjct: 343 DADGSSSFRRAFQRQEEKWWLPVPRVPPGGLHENTKKQLQHKRDCTSQILKAAMAINNIT 402

Query: 303 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 362
           LAD+E+PE YLEALPKNGRA LGDLIYRYISSD FSPECLLDCL++SSEHQA EIANRVE
Sbjct: 403 LADMEVPESYLEALPKNGRASLGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVE 462

Query: 363 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 422
           ASIY+WRK+ N KP  N T+ SS+SSWEMVKELMVD+EK+ELL ERAE+LL+CL+QRFPG
Sbjct: 463 ASIYLWRKKANLKPTNNATRFSSRSSWEMVKELMVDAEKKELLPERAESLLMCLRQRFPG 522

Query: 423 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 482
           LPQT LDMSKIQYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLT+HSD Q SSI
Sbjct: 523 LPQTALDMSKIQYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTRHSD-QFSSI 582

Query: 483 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 542
           SK+ +ISHK++P  + VPIS TPYKT F TPSFSP Q  SP +G+RSPFI   KIPQRG 
Sbjct: 583 SKVSIISHKSTPNPYTVPISSTPYKTAFTTPSFSPGQLVSPLRGDRSPFITSGKIPQRGL 642

Query: 543 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQ 582
           GVKKVLTDYLS++A+ GK+  + IEK + ++N+  E   SQ
Sbjct: 643 GVKKVLTDYLSIDAK-GKDYGDPIEKPDSVSNTMQETLGSQ 681

BLAST of Cucsa.066670 vs. NCBI nr
Match: gi|566175372|ref|XP_002309095.2| (hypothetical protein POPTR_0006s09370g [Populus trichocarpa])

HSP 1 Score: 863.2 bits (2229), Expect = 2.6e-247
Identity = 440/594 (74.07%), Postives = 496/594 (83.50%), Query Frame = 1

Query: 7   EKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSED-SSSPSSSS 66
           EK    G    E +T G LIE+  RESSSSSDFLTSE TG EEH HS SE+ SSSP +  
Sbjct: 14  EKDTNFGEKKGEVQTFGDLIEDKCRESSSSSDFLTSEATGNEEHGHSSSEEGSSSPHTMG 73

Query: 67  WSTRKAEAPDCIKANGVVNGD-KNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGC 126
           W   K EAPDC   +G  + + K+H D+RKL K+ SS SE EMMKERFSKLLLGEDMSGC
Sbjct: 74  WPVLKDEAPDCTNTDGATDDEEKSHFDDRKLGKQGSSVSETEMMKERFSKLLLGEDMSGC 133

Query: 127 GNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVELTPTW 186
           GNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK MWRREM+WLLCVSDHIVEL P+W
Sbjct: 134 GNGVCTALAISNAITNLCATLFGQLWRLEPLPLEKKAMWRREMEWLLCVSDHIVELMPSW 193

Query: 187 QTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAPETDG 246
           QTFPDGSKLEVMTC PRSDLY+NLPALRKLDNMLLEILDSF++ EFWYVDQGILAP+TDG
Sbjct: 194 QTFPDGSKLEVMTCGPRSDLYINLPALRKLDNMLLEILDSFDNTEFWYVDQGILAPDTDG 253

Query: 247 SSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTTLADL 306
           S+S  R LQRQEEKWWLPVP+VPPGGL ENSR++LQHKR+STNQILKAAMAINS TLA++
Sbjct: 254 SASFRRTLQRQEEKWWLPVPQVPPGGLHENSRKKLQHKRDSTNQILKAAMAINSITLAEM 313

Query: 307 EIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVEASIY 366
           EIPE YLEALPKNG+A LGDLIYRYISSD F PECLLDCL++SSEHQA E+ANRVEASIY
Sbjct: 314 EIPESYLEALPKNGKASLGDLIYRYISSDQFYPECLLDCLDLSSEHQAIELANRVEASIY 373

Query: 367 VWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQT 426
           +WRKRTN KPA++T + SSKSSWE+VKELM+D++KRELLA+RAE+LLLCLKQRFPGLPQT
Sbjct: 374 IWRKRTNYKPASSTNRSSSKSSWELVKELMIDADKRELLADRAESLLLCLKQRFPGLPQT 433

Query: 427 TLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSISKLG 486
           TLDMSKIQYNKDVGKSILESYSRVLESLAFN+VARIDDLLYVDDLTKHSDH  SSISK+ 
Sbjct: 434 TLDMSKIQYNKDVGKSILESYSRVLESLAFNIVARIDDLLYVDDLTKHSDH-FSSISKVS 493

Query: 487 VISHKNSPLHFPVPISGTPYKTPFATPSFSPA-QTASPAKGERSPFIPGTKIPQRGFGVK 546
           VI+HK+  + + VP+S TPYKT F TPSFSP  Q  SPAKG+RSPF+   KIPQRG GVK
Sbjct: 494 VIAHKSVTIPYSVPVSNTPYKTAFNTPSFSPGQQRISPAKGDRSPFMTSGKIPQRGLGVK 553

Query: 547 KVLTDYLSVEARGGKELSNKIEKAE-PITNSKLERQASQYRGGV---ASSPRQR 594
           KVLT+YLS++   G++  N IE A+  I N+   +   +  G +    SSP  R
Sbjct: 554 KVLTEYLSIDTE-GRDGGNMIEGADNVIRNTSASQTGVESFGSIIETISSPENR 605