Cla97C05G103790 (gene) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C05G103790
Type	gene
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase
Location	Cla97Chr05 : 31845919 .. 31846830 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGCCCGCCGCATTCCTCTTCTCCCCGTTTTCTTCTATTTTCCTCCTCTCTCTCTCTTCCCTCACCTTCCTTTCCATTCTCCTCCTGGCTTTCGCTGAGTTCATCGGCCACCACTTACAGTATTCCAAATTCTGGACTCCTAATTCCAAATTCCATCTCCCTTCCCGTTCCGCCATGCTTCTCTGTTACCTCCCGGCTTTCTCCCTCGCTTCCATCTCCTCTCTCTTCCTCTTCCCTCACCACTTTCTTCACTTCTCCAGGATTTTCCTCATCAATTCCCTTCTCCTCATCCATTTCTTCAAACGGATCTTCGAGGTACGTCTCTGCAATCAATCAATCAATTAAGTGTTTAAATAAGGTAGTTTTTCAATTTTTTTTGTAAAAAAAATAAATATGTAATTAAATGCGGGCTATTTCAATCTCCATCGTTTTCAGGTTCTGTTCGTTCACAAATACAGCAACAAGATGGCTCTTAACGACGCGATTATCACCTCAGTTGCCTACCTACTCTTTACCGGCAGTACGATCTACGCTCAGATGGTTTCGCTGGATCTGCCGGAGCCGGAGATCGATCTGAAGGCGGCGGGATTGGCGCTGTACGGGATTGGAATCGCCGGTAATTTCTATCACCATATTCTACTGTCGAGATTGAGAAGAGAAGGCGATCGAGAGTACAGGATTCCGAGAGGAGGATTGTTTGAACTGATCGTGTGTCCTCACTACCTATTTGAGATTGTAGAATTTCTTGGAATCGCTTTGGTTTCGCAGACGTTGTTCGCGGTGGCGTTCGTGTTCGGATCGGCGTTGTTCTTGATTGGAAGAAGCTACGCCACCAGGAATTGGTACCTTTCCAAATTTGAAGATTTTCCTCGCCATGTTAAGGCCATTCTTCCGTTTGTTTTCTGA

mRNA sequence

ATGGCGTTGCCCGCCGCATTCCTCTTCTCCCCGTTTTCTTCTATTTTCCTCCTCTCTCTCTCTTCCCTCACCTTCCTTTCCATTCTCCTCCTGGCTTTCGCTGAGTTCATCGGCCACCACTTACAGTATTCCAAATTCTGGACTCCTAATTCCAAATTCCATCTCCCTTCCCGTTCCGCCATGCTTCTCTGTTACCTCCCGGCTTTCTCCCTCGCTTCCATCTCCTCTCTCTTCCTCTTCCCTCACCACTTTCTTCACTTCTCCAGGATTTTCCTCATCAATTCCCTTCTCCTCATCCATTTCTTCAAACGGATCTTCGAGGTTCTGTTCGTTCACAAATACAGCAACAAGATGGCTCTTAACGACGCGATTATCACCTCAGTTGCCTACCTACTCTTTACCGGCAGTACGATCTACGCTCAGATGGTTTCGCTGGATCTGCCGGAGCCGGAGATCGATCTGAAGGCGGCGGGATTGGCGCTGTACGGGATTGGAATCGCCGGTAATTTCTATCACCATATTCTACTGTCGAGATTGAGAAGAGAAGGCGATCGAGAGTACAGGATTCCGAGAGGAGGATTGTTTGAACTGATCGTGTGTCCTCACTACCTATTTGAGATTGTAGAATTTCTTGGAATCGCTTTGGTTTCGCAGACGTTGTTCGCGGTGGCGTTCGTGTTCGGATCGGCGTTGTTCTTGATTGGAAGAAGCTACGCCACCAGGAATTGGTACCTTTCCAAATTTGAAGATTTTCCTCGCCATGTTAAGGCCATTCTTCCGTTTGTTTTCTGA

Coding sequence (CDS)

ATGGCGTTGCCCGCCGCATTCCTCTTCTCCCCGTTTTCTTCTATTTTCCTCCTCTCTCTCTCTTCCCTCACCTTCCTTTCCATTCTCCTCCTGGCTTTCGCTGAGTTCATCGGCCACCACTTACAGTATTCCAAATTCTGGACTCCTAATTCCAAATTCCATCTCCCTTCCCGTTCCGCCATGCTTCTCTGTTACCTCCCGGCTTTCTCCCTCGCTTCCATCTCCTCTCTCTTCCTCTTCCCTCACCACTTTCTTCACTTCTCCAGGATTTTCCTCATCAATTCCCTTCTCCTCATCCATTTCTTCAAACGGATCTTCGAGGTTCTGTTCGTTCACAAATACAGCAACAAGATGGCTCTTAACGACGCGATTATCACCTCAGTTGCCTACCTACTCTTTACCGGCAGTACGATCTACGCTCAGATGGTTTCGCTGGATCTGCCGGAGCCGGAGATCGATCTGAAGGCGGCGGGATTGGCGCTGTACGGGATTGGAATCGCCGGTAATTTCTATCACCATATTCTACTGTCGAGATTGAGAAGAGAAGGCGATCGAGAGTACAGGATTCCGAGAGGAGGATTGTTTGAACTGATCGTGTGTCCTCACTACCTATTTGAGATTGTAGAATTTCTTGGAATCGCTTTGGTTTCGCAGACGTTGTTCGCGGTGGCGTTCGTGTTCGGATCGGCGTTGTTCTTGATTGGAAGAAGCTACGCCACCAGGAATTGGTACCTTTCCAAATTTGAAGATTTTCCTCGCCATGTTAAGGCCATTCTTCCGTTTGTTTTCTGA

Protein sequence

MALPAAFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPNSKFHLPSRSAMLLCYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF

Homology

[Close]

BLAST of Cla97C05G103790 vs. NCBI nr
Match: XP_008446721.1 (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo])

HSP 1 Score: 421.8 bits (1083), Expect = 1.7e-114
Identity = 223/258 (86.43%), Postives = 234/258 (90.70%), Query Frame = 0


Query: 6   AFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPNSKFHLPSRSAMLLCY 65
           AFLF PFSS+FLLSLSSLTFLSIL L   E +GH L+YSKFWTPNSKFHLPSRSAMLLCY
Sbjct: 5   AFLFYPFSSLFLLSLSSLTFLSILFLVSGELLGHPLRYSKFWTPNSKFHLPSRSAMLLCY 64

Query: 66  LPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAII 125
           LPAFSL+S+ SLFL PHHFL  SRIFLI SLLL HFFKRI EVLFVHKYSNKMAL DAII
Sbjct: 65  LPAFSLSSL-SLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDAII 124

Query: 126 TSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDR 185
           TS+AYLLFTG+TIYAQ+VSLDL EPEIDLKAAGLALYGIGI GNFYHH+LLSRLR  GDR
Sbjct: 125 TSLAYLLFTGATIYAQIVSLDLVEPEIDLKAAGLALYGIGITGNFYHHVLLSRLRSGGDR 184

Query: 186 EYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYL 245
           EYRIPRGGLF++IVCPHYLFEIVEFLGIA VSQTLFAV FV GSALFLIGRSYATR WYL
Sbjct: 185 EYRIPRGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFAVVFVVGSALFLIGRSYATRKWYL 244

Query: 246 SKFEDFPRHVKAILPFVF 264
           SKFEDFP HVKAILPFVF
Sbjct: 245 SKFEDFPTHVKAILPFVF 261

[Close]

BLAST of Cla97C05G103790 vs. NCBI nr
Match: XP_004135372.1 (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis sativus] >KGN52157.1 hypothetical protein Csa_5G612860 [Cucumis sativus])

HSP 1 Score: 407.1 bits (1045), Expect = 4.4e-110
Identity = 213/258 (82.56%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 6   AFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPNSKFHLPSRSAMLLCY 65
           AFLFSPFSS+FLLSLSSLTFLSILLL   EF+GH L+YSKFWTPNSK HLPSRSAMLLCY
Sbjct: 5   AFLFSPFSSLFLLSLSSLTFLSILLLVSGEFLGHPLRYSKFWTPNSKPHLPSRSAMLLCY 64

Query: 66  LPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAII 125
           LPAF      SLFL PHHFL  SRIFLI SLLL HFFKRI EVLFVHKYSNKMAL DAI+
Sbjct: 65  LPAF------SLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDAIM 124

Query: 126 TSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDR 185
           TS++YLLF+G+TIYAQ+V+LDL EPEIDLKAAGLA+YGIGI GNFYHH+LLSRLR  GDR
Sbjct: 125 TSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGGDR 184

Query: 186 EYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYL 245
           EY+IP+GGLF++IVCPHYLFEIVEFLGIA VSQTLF V FV GSALFLIGRSYATR WYL
Sbjct: 185 EYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRSYATRKWYL 244

Query: 246 SKFEDFPRHVKAILPFVF 264
           SKFEDFP H+KAILPFVF
Sbjct: 245 SKFEDFPSHIKAILPFVF 256

[Close]

BLAST of Cla97C05G103790 vs. NCBI nr
Match: XP_022985155.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima])

HSP 1 Score: 387.1 bits (993), Expect = 4.7e-104
Identity = 198/260 (76.15%), Postives = 228/260 (87.69%), Query Frame = 0


Query: 7   FLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTP---NSKFHLPSRSAMLL 66
           FLFSPFSS F+L+LSSL  LS L+LA +EF+G HLQYSKFW+P   ++K HLPSRSAMLL
Sbjct: 4   FLFSPFSSPFILALSSLIMLSTLVLATSEFLGRHLQYSKFWSPKNSSNKIHLPSRSAMLL 63

Query: 67  CYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDA 126
           CY+PAF++ASIS   L P +    +RIFLINSLLLIHFFKRIFEV+FVHKYSNKM L+DA
Sbjct: 64  CYVPAFAVASISLFLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYSNKMGLSDA 123

Query: 127 IITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREG 186
           II S+ Y+L TGSTIY+QMV+ DL EP+IDL+AAGLA+Y IGI GN YHH+LLS+LR+EG
Sbjct: 124 IIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLSKLRKEG 183

Query: 187 DREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNW 246
           DREYRIPRGG+FE+IVCPHYLFE+VEFLGIA VSQTLFA+ F  GSALFLIGRSYATRNW
Sbjct: 184 DREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRSYATRNW 243

Query: 247 YLSKFEDFPRHVKAILPFVF 264
           Y+SKFEDFPRHVKAILPFVF
Sbjct: 244 YVSKFEDFPRHVKAILPFVF 263

[Close]

BLAST of Cla97C05G103790 vs. NCBI nr
Match: XP_023552351.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.2 bits (975), Expect = 5.8e-102
Identity = 199/261 (76.25%), Postives = 228/261 (87.36%), Query Frame = 0


Query: 7   FLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTP---NSKFHLPSRSAMLL 66
           FLFSPFSS FLL+LSSLT LSIL+LA +EF+G HLQYSKFW+P   ++K HLPSRSAMLL
Sbjct: 4   FLFSPFSSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLL 63

Query: 67  CYLPAFSLASIS-SLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALND 126
           CY+PAF++ASIS  L L P +    +RIFLINSLLLIHFFKRIFEVLFVHKYSNKM L+D
Sbjct: 64  CYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSD 123

Query: 127 AIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRRE 186
           AI  S+ Y+L T STI +QMVS +L EP+IDL+AAGLA+Y IGI GN YHH+LL++LR+E
Sbjct: 124 AIRVSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKE 183

Query: 187 GDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRN 246
           GDREYRIPRGG+FE+IVCPHYLFE+VEFLGIA VSQTLF + F FGSALFLIGRSYATRN
Sbjct: 184 GDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRN 243

Query: 247 WYLSKFEDFPRHVKAILPFVF 264
           WY+SKFEDFPRHVKAILPFVF
Sbjct: 244 WYVSKFEDFPRHVKAILPFVF 264

[Close]

BLAST of Cla97C05G103790 vs. NCBI nr
Match: XP_022957136.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 378.3 bits (970), Expect = 2.2e-101
Identity = 196/261 (75.10%), Postives = 228/261 (87.36%), Query Frame = 0


Query: 7   FLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTP---NSKFHLPSRSAMLL 66
           FLFSPFSS FLL+LSSLT LSIL+LA +EF+G HLQYSKFW+P   ++K HLPSRSAMLL
Sbjct: 4   FLFSPFSSPFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLL 63

Query: 67  CYLPAFSLASIS-SLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALND 126
           CY+PAF++ASIS  L L P +    +RIFLINSLLLIHFFKRIFEV+FVHKY NKM L++
Sbjct: 64  CYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYGNKMGLSE 123

Query: 127 AIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRRE 186
           AI  S+ Y+L T STIY+QMVS +L EP+IDL+AAGLA+Y IGI GN YHH+LL++LR+E
Sbjct: 124 AIRVSITYVLLTASTIYSQMVSQNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKE 183

Query: 187 GDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRN 246
           GDREYRIPRGG+FE+IVCPHYLFE+VEFLG+A VSQTLF + F FGSALFLIGRSYATRN
Sbjct: 184 GDREYRIPRGGMFEVIVCPHYLFEMVEFLGLAFVSQTLFPMVFAFGSALFLIGRSYATRN 243

Query: 247 WYLSKFEDFPRHVKAILPFVF 264
           WY+SKFEDFPRHVKAILPFVF
Sbjct: 244 WYVSKFEDFPRHVKAILPFVF 264

[Close]

BLAST of Cla97C05G103790 vs. TrEMBL
Match: tr|A0A1S3BFS4|A0A1S3BFS4_CUCME (steroid 5-alpha-reductase DET2-like OS=Cucumis melo OX=3656 GN=LOC103489358 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.1e-114
Identity = 223/258 (86.43%), Postives = 234/258 (90.70%), Query Frame = 0


Query: 6   AFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPNSKFHLPSRSAMLLCY 65
           AFLF PFSS+FLLSLSSLTFLSIL L   E +GH L+YSKFWTPNSKFHLPSRSAMLLCY
Sbjct: 5   AFLFYPFSSLFLLSLSSLTFLSILFLVSGELLGHPLRYSKFWTPNSKFHLPSRSAMLLCY 64

Query: 66  LPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAII 125
           LPAFSL+S+ SLFL PHHFL  SRIFLI SLLL HFFKRI EVLFVHKYSNKMAL DAII
Sbjct: 65  LPAFSLSSL-SLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDAII 124

Query: 126 TSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDR 185
           TS+AYLLFTG+TIYAQ+VSLDL EPEIDLKAAGLALYGIGI GNFYHH+LLSRLR  GDR
Sbjct: 125 TSLAYLLFTGATIYAQIVSLDLVEPEIDLKAAGLALYGIGITGNFYHHVLLSRLRSGGDR 184

Query: 186 EYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYL 245
           EYRIPRGGLF++IVCPHYLFEIVEFLGIA VSQTLFAV FV GSALFLIGRSYATR WYL
Sbjct: 185 EYRIPRGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFAVVFVVGSALFLIGRSYATRKWYL 244

Query: 246 SKFEDFPRHVKAILPFVF 264
           SKFEDFP HVKAILPFVF
Sbjct: 245 SKFEDFPTHVKAILPFVF 261

[Close]

BLAST of Cla97C05G103790 vs. TrEMBL
Match: tr|A0A0A0KUP2|A0A0A0KUP2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G612860 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.9e-110
Identity = 213/258 (82.56%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 6   AFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPNSKFHLPSRSAMLLCY 65
           AFLFSPFSS+FLLSLSSLTFLSILLL   EF+GH L+YSKFWTPNSK HLPSRSAMLLCY
Sbjct: 5   AFLFSPFSSLFLLSLSSLTFLSILLLVSGEFLGHPLRYSKFWTPNSKPHLPSRSAMLLCY 64

Query: 66  LPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAII 125
           LPAF      SLFL PHHFL  SRIFLI SLLL HFFKRI EVLFVHKYSNKMAL DAI+
Sbjct: 65  LPAF------SLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDAIM 124

Query: 126 TSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDR 185
           TS++YLLF+G+TIYAQ+V+LDL EPEIDLKAAGLA+YGIGI GNFYHH+LLSRLR  GDR
Sbjct: 125 TSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGGDR 184

Query: 186 EYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYL 245
           EY+IP+GGLF++IVCPHYLFEIVEFLGIA VSQTLF V FV GSALFLIGRSYATR WYL
Sbjct: 185 EYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRSYATRKWYL 244

Query: 246 SKFEDFPRHVKAILPFVF 264
           SKFEDFP H+KAILPFVF
Sbjct: 245 SKFEDFPSHIKAILPFVF 256

[Close]

BLAST of Cla97C05G103790 vs. TrEMBL
Match: tr|A0A2I4H7C8|A0A2I4H7C8_9ROSI (steroid 5-alpha-reductase DET2-like OS=Juglans regia OX=51240 GN=LOC109014156 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 143/267 (53.56%), Postives = 194/267 (72.66%), Query Frame = 0


Query: 7   FLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFW---TPNS-------KFHLP 66
           F+F P +S+F+ ++S ++  S+    F+E    HLQYSKFW   +P S       +  L 
Sbjct: 9   FIFPPPNSLFVTAMSVISLTSLAYTGFSEVRERHLQYSKFWNVISPESGTRRKGKQIKLS 68

Query: 67  SRSAMLLCYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSN 126
           SR+ MLL Y PAF LA ++S  +FPH  L   R+ L++S L +HFFKR+FEVLFVHKYS 
Sbjct: 69  SRTGMLLLYTPAF-LAGLTSFVIFPHQDL---RLLLLDSALTLHFFKRVFEVLFVHKYSG 128

Query: 127 KMALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILL 186
            M L+ AI  +++YLL T + IYAQ ++  +PEP +DL   G+ L+ IGI+GNFYHH LL
Sbjct: 129 SMVLDSAIPITLSYLLSTATMIYAQHLTQGMPEPPVDLMYPGVLLFLIGISGNFYHHFLL 188

Query: 187 SRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGR 246
           S+LR EGD+EYRIP+GGLF+L++CPHYLFEI++F+GI+ +SQT +A +F  G+  +L+GR
Sbjct: 189 SKLRGEGDKEYRIPKGGLFDLVLCPHYLFEIIDFIGISFISQTWYAFSFTLGTIFYLMGR 248

Query: 247 SYATRNWYLSKFEDFPRHVKAILPFVF 264
           SYATR WYLSKFEDFP  VKA++PFVF
Sbjct: 249 SYATRRWYLSKFEDFPEDVKALIPFVF 271

[Close]

BLAST of Cla97C05G103790 vs. TrEMBL
Match: tr|A0A2I4HIX5|A0A2I4HIX5_9ROSI (steroid 5-alpha-reductase DET2-like OS=Juglans regia OX=51240 GN=LOC109018421 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.5e-69
Identity = 142/267 (53.18%), Postives = 193/267 (72.28%), Query Frame = 0


Query: 7   FLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFW---TPNS-------KFHLP 66
           F+F P +S+F+ ++S ++  S+    F+E    HLQYSKFW   +P S       +  L 
Sbjct: 9   FIFPPPNSLFVTAMSVISLTSLAYTGFSEVRERHLQYSKFWNVISPESGTRRKGKQIKLS 68

Query: 67  SRSAMLLCYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSN 126
           SR+ MLL Y PAF LA ++S  +FPH  L   R+ L++S L +HFFKR+FEVLFVHKYS 
Sbjct: 69  SRTGMLLLYTPAF-LAGLTSFVIFPHQDL---RLLLLDSALTLHFFKRVFEVLFVHKYSG 128

Query: 127 KMALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILL 186
            M L+ AI  +++YLL T + IYAQ ++  +PEP +DL   G+ L+ IGI+GNFYHH LL
Sbjct: 129 SMVLDSAIPITLSYLLSTATMIYAQHLTQGMPEPPVDLMYPGVLLFLIGISGNFYHHFLL 188

Query: 187 SRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGR 246
           S+LR EGD+EYRIP+GGLF+L++C HYLFEI++F+GI+ +SQT +A +F  G+  +L+GR
Sbjct: 189 SKLRGEGDKEYRIPKGGLFDLVLCQHYLFEIIDFIGISFISQTWYAFSFTLGTIFYLMGR 248

Query: 247 SYATRNWYLSKFEDFPRHVKAILPFVF 264
           SYATR WYLSKFEDFP  VKA++PFVF
Sbjct: 249 SYATRRWYLSKFEDFPEDVKALIPFVF 271

[Close]

BLAST of Cla97C05G103790 vs. TrEMBL
Match: tr|I1JDE1|I1JDE1_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100798454 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.3e-69
Identity = 138/262 (52.67%), Postives = 189/262 (72.14%), Query Frame = 0


Query: 6   AFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPN----SKFHLPSRSAM 65
           +F+F P SS+ +  +S ++ +S+    F+E  G HL YSKFW  N     +  L S++ M
Sbjct: 9   SFIFPPPSSLVVSGMSVISLVSLANAGFSEIRGKHLNYSKFWNANPSAEKQVKLSSKAGM 68

Query: 66  LLCYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALN 125
           LL Y PAF LA ++S ++FPH  L   R  L+ S + +HFFKR+FEV+F+HKYS  M L+
Sbjct: 69  LLLYTPAF-LAGLASFWIFPHQGL---RSTLLQSAVTLHFFKRVFEVVFIHKYSGAMLLD 128

Query: 126 DAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRR 185
            AI  +++Y L T + IYAQ ++  LPEP IDL   G+ L+ +GI GNFYHH LLS LR 
Sbjct: 129 SAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLSNLRG 188

Query: 186 EGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATR 245
           +G++EY+IP+GG+FEL++CPHYLFEI+EF G + +SQTL+A +F  G+ L+L+GRSY+TR
Sbjct: 189 KGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLGRSYSTR 248

Query: 246 NWYLSKFEDFPRHVKAILPFVF 264
            WYLSKFEDFP HVKAI+PFVF
Sbjct: 249 KWYLSKFEDFPEHVKAIIPFVF 266

[Close]

BLAST of Cla97C05G103790 vs. Swiss-Prot
Match: sp|Q2QDF6|DET2_GOSHI (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-17
Identity = 64/200 (32.00%), Postives = 108/200 (54.00%), Query Frame = 0


Query: 76  SLFLFP--HHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLF 135
           + FLFP   HF +  + FL+ S  L H+F R   VL+  + +           SVA++ F
Sbjct: 62  TFFLFPSGQHFYN-PKSFLLISPFLFHYFNR--TVLYPLRLARNTTQTRGFPVSVAFMAF 121

Query: 136 TGSTIYAQMVSLDLPEPEIDLK---------AAGLALYGIGIAGNFYHHILLSRLRREGD 195
             + +   + +  +   + D +          AGL ++ +G+  N     +L  L+++GD
Sbjct: 122 GFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGD 181

Query: 196 REYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWY 255
             Y+IPRGGLFEL+ CP+Y  EI+E+ G A+++ +     F   +   L+ R+ ATR WY
Sbjct: 182 GGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWY 241

Query: 256 LSKF-EDFPRHVKAILPFVF 264
           L KF +D+P+  KA++PF++
Sbjct: 242 LEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of Cla97C05G103790 vs. Swiss-Prot
Match: sp|Q28891|S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.3e-16
Identity = 66/230 (28.70%), Postives = 117/230 (50.87%), Query Frame = 0


Query: 40  HLQYSKFWTPNSKFHLPSRSAMLLCYLPAFSLASISSLFLFPHHF---LHFSRIFLINSL 99
           +L Y +  +P+ +  +P+R+A ++  LP+ +L     L+ +       L  +   ++ ++
Sbjct: 39  NLAYGRHASPSFRVRVPARAAWVVQELPSLAL----PLYQYASESAPRLRSAPNCILLAM 98

Query: 100 LLIHFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLFTG--STIYAQMVSLDLPEPEIDL 159
            L+H+  R     F+ +    M L  A   ++ +  F G   + Y    ++   +   D 
Sbjct: 99  FLVHYGHRCLIYPFLMRGGKPMPLL-ACTMAIMFCTFNGYLQSRYLSHWAVYADDWVTDP 158

Query: 160 K-AAGLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGI 219
           +   G  L+  G+  N +   +L  LR+ GD  Y+IPRGGLFE +   +Y  EI+E+ G 
Sbjct: 159 RFLIGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGY 218

Query: 220 ALVSQTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 264
           AL S ++   AF F +  FL GR+     WYL KFE++P+  K ++PF+F
Sbjct: 219 ALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of Cla97C05G103790 vs. Swiss-Prot
Match: sp|P31213|S5A2_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 68/214 (31.78%), Postives = 115/214 (53.74%), Query Frame = 0


Query: 55  LPSRSAMLLCYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKY 114
           LP+R+A  L  LP+F++ +   L   P          L+  L  +H+F R     FV+  
Sbjct: 47  LPARAAWFLQELPSFAVPA-GILARQPLSLFGPPGTVLL-GLFCVHYFHR----TFVYSL 106

Query: 115 SNKMALNDAIITSVAYLLFTGSTI---YAQMVSLDLPEP-EIDLK-AAGLALYGIGIAGN 174
            N+     AI+        TG+ +   Y  +   + P+    D++ + G+ L+ +G+  N
Sbjct: 107 LNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGIN 166

Query: 175 FYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGS 234
            +   +L +LR+ G+  YRIP+GGLF  +   ++L EI+E++G AL + +L A+AF F S
Sbjct: 167 IHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFS 226

Query: 235 ALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 264
             FL  R++    +YL  FED+P+  KA++PF+F
Sbjct: 227 LCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of Cla97C05G103790 vs. Swiss-Prot
Match: sp|P18405|S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.8e-15
Identity = 66/233 (28.33%), Postives = 111/233 (47.64%), Query Frame = 0


Query: 43  YSKFWTPNSKFHLPSRSAMLLCYLPAFSLASISSLFLFPHHF---LHFSRIFLINSLLLI 102
           Y +   P+ +  +P+R+A ++  LP+ +L     L+ +       L  +   ++ ++ L+
Sbjct: 38  YGRHALPSHRLRVPARAAWVVQELPSLAL----PLYQYASESAPRLRSAPNCILLAMFLV 97

Query: 103 HFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLFTGSTIYAQMVSLD---------LPEP 162
           H+  R     F+ +    M L    +     ++F     Y Q   L          + +P
Sbjct: 98  HYGHRCLIYPFLMRGGKPMPL----LACTMAIMFCTCNGYLQSRYLSHCAVYADDWVTDP 157

Query: 163 EIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEF 222
                  G  L+  G+  N +   +L  LR+ GD  Y+IPRGGLFE +   +Y  EI+E+
Sbjct: 158 RF---LIGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEW 217

Query: 223 LGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 264
            G AL S ++   AF F +  FL GR+     WYL KFE++P+  K I+PF+F
Sbjct: 218 CGYALASWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of Cla97C05G103790 vs. Swiss-Prot
Match: sp|A5PJS2|S5A1_BOVIN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus OX=9913 GN=SRD5A1 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.4e-14
Identity = 66/226 (29.20%), Postives = 110/226 (48.67%), Query Frame = 0


Query: 43  YSKFWTPNSKFHLPSRSAMLLCYLPAFSLASISSLFL-FPHHFLHFSRIFLINSLLLIHF 102
           Y ++ +  S F LP+R+A  +  LP+ +L  ++      P   L+     ++ ++ L+H+
Sbjct: 34  YGRYASSGSAFGLPARAAWTVQELPSLALPLLACAGAGAPAERLNRWPNCILLAMFLVHY 93

Query: 103 FKRIFEVLFVHKYSNKMALNDAIITSV-----AYLLFTGSTIYAQMVSLDLPEPEIDLKA 162
            +R     F+ +    M L   ++  +      YL     + YA      L +P      
Sbjct: 94  AQRSLVFPFLIRGGKPMPLYAFLLAFIFCTYNGYLQSRYLSQYAVYADDWLSDPRF---L 153

Query: 163 AGLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALV 222
            G AL+ IG+  N +   +L  LR+ G+  Y+IPRGGLFE I   +Y  E+VE+ G AL 
Sbjct: 154 TGSALWLIGMLINIHSDHVLRNLRKPGETGYKIPRGGLFEYISAANYFGEVVEWCGYALA 213

Query: 223 SQTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFV 263
           S ++   AF   +   L  R+     WY  KFED+P+  K ++PF+
Sbjct: 214 SWSIQGWAFAVFTFCVLFTRAQQHHKWYHEKFEDYPKFRKIMIPFL 256

[Close]

BLAST of Cla97C05G103790 vs. TAIR10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 242.7 bits (618), Expect = 2.6e-64
Identity = 129/272 (47.43%), Postives = 187/272 (68.75%), Query Frame = 0


Query: 1   MALPAAFLFSPFSSIFLLSLSSLTFLSILLLAFAEFIGHHLQYSKFWTPNSK-------- 60
           M +  +F++ P  SI L  +S +   ++  + ++E  G+HL+YSKF   +S         
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  FHLPSRSAMLLCYLPAFSLASISSLFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVH 120
             + SR+ MLL Y PAF LA+ SS F+ P   L F    L+ S L +HFFKR+FEVLF+H
Sbjct: 61  GSISSRNGMLLLYTPAF-LAAASSFFVVPSDDLRF---LLLKSALALHFFKRVFEVLFIH 120

Query: 121 KYSNKMALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYH 180
           KYS  MA++ A+  S +Y   T   +Y+Q ++L L EP  D+K AG+ ++ +GI GN YH
Sbjct: 121 KYSGGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYH 180

Query: 181 HILLSRLRRE-GDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSAL 240
           H+LL++LR+E G +EY+IP+GGLF++I+CPHYLFEI+ F    L+SQT+++ +F  G+ L
Sbjct: 181 HVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTML 240

Query: 241 FLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 264
           +LIGRSYATR WYLSKF+DFP+H+KA++PFVF
Sbjct: 241 YLIGRSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of Cla97C05G103790 vs. TAIR10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 74.7 bits (182), Expect = 9.4e-14
Identity = 58/197 (29.44%), Postives = 100/197 (50.76%), Query Frame = 0


Query: 77  LFLFPHHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLFTGS 136
           LF F  H L+   + L +  L+ +F + I   L + + S     N   IT +A L FT +
Sbjct: 67  LFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPIT-IAALAFTFN 126

Query: 137 TIYAQMVSLDLPEPEIDLK---------AAGLALYGIGIAGNFYHHILLSRLRREGDREY 196
            +   + +  +   + D +           G+ ++  G+  N      L RL++E    Y
Sbjct: 127 LLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGGY 186

Query: 197 RIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYLSK 256
            IPRGG FEL+ CP+Y  E +E+LG A+++ +   + F   +   L  R+ A+  WY++K
Sbjct: 187 VIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAK 246

Query: 257 F-EDFPRHVKAILPFVF 264
           F E++P+  KA++PFV+
Sbjct: 247 FKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Cla97C05G103790 vs. TAIR10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 74.7 bits (182), Expect = 9.4e-14
Identity = 54/152 (35.53%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 100 HFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGL 159
           H+FKRI E  FVH++S+  +    +  + AY    G+ I A  V+  L  P  DL+    
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMK-- 200

Query: 160 ALYGIGI---AGNFYHHILLSRLR-REGDREYRIPRGGLFELIVCPHYLFEIVEFLGIAL 219
             +G G+     NFY HILL  LR   G   Y+IPRG LF ++ C +Y  EI ++LG  +
Sbjct: 201 IGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNI 260

Query: 220 VSQTLFAVAFVFGSALFLIGRSYATRNWYLSK 248
            +QT+    F+  +AL +        NW L K
Sbjct: 261 ATQTIAGYVFLAVAALIM-------TNWALGK 282

[Close]

BLAST of Cla97C05G103790 vs. TAIR10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 85/307 (27.69%), Postives = 132/307 (43.00%), Query Frame = 0


Query: 14  SIFLLSLSSLTFLSI---LLLAFAEFIGHHLQYSKFWTPNS-KFHLPSR----------- 73
           +++++S+  L   SI    L +F E +       K   P+S KF +P +           
Sbjct: 15  TVWIVSILPLVIASIPSSKLNSFRELVLSFAGRGKILHPSSQKFTVPQKFFGHFYVVGVV 74

Query: 74  -SAMLLC--YLPAFSLASISSLFLF-----PHHFLHFSRIFLINSLLLIHFFKRIFEVLF 133
            + +LL   ++ A  +A  S +F F      H F     +FL+  L+ IH  +R+ E  +
Sbjct: 75  WTTLLLAATWMYACKMAGGSHVFSFHMTHVEHRFKVGRAVFLL-LLMEIHVLRRVIESFY 134

Query: 134 VHKYSNK-----MALNDAIITSVAYLL------------FTGSTIYAQMVSLDLPEPEID 193
           V KYS       +A   A+   VA  L            F GS +   + S      + +
Sbjct: 135 VFKYSTSARMHILAYVGALFYYVAAPLSLCSNIAPEVARFVGSQVAEFIASGKSHSHDFN 194

Query: 194 LKAAGLALYGIG----IAGNFY---------HHILLSRLRR--EGDREYRIPRGGLFELI 253
           L  +   L  +G    I G  +          H +L  LR      +EY IP G  FE++
Sbjct: 195 LLLSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMV 254

Query: 254 VCPHYLFEIVEFLGIALVS-QTLFAVAFVFG-SALFLIGRSYATRNWYLSKFEDFPRHVK 264
            CPH+L EIV +LG+ + S  T  ++  +FG  A  L   +  T  WYL KFE++P    
Sbjct: 255 SCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASRH 314

[Close]

BLAST of Cla97C05G103790 vs. TAIR10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 52.4 bits (124), Expect = 5.0e-07
Identity = 62/221 (28.05%), Postives = 93/221 (42.08%), Query Frame = 0


Query: 82  HHFLHFSRIFLINSLLLIHFFKRIFEVLFVHKYSNKMALNDAIITSVAYLLFTGSTIYAQ 141
           H F  +  +FL+  L+ IH  +R+ E  +V KYS    ++   I      LF   T    
Sbjct: 127 HRFKVWRAVFLL-LLMEIHVLRRLIESFYVFKYSPSARMH---ILGYFAGLFFYVTAPLS 186

Query: 142 MVSLDLPE----------------------PEIDLKAAGLALYGIG----IAGNFY---- 201
           + S   PE                      PE +L ++   L  +G    I G  +    
Sbjct: 187 LCSNIAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWGW 246

Query: 202 -----HHILLSRLRREGD--REYRIPRGGLFELIVCPHYLFEIVEFLGIALVS-QTLFAV 261
                 H +L  LR      +EY IP G  F ++  PH+L EIV + G+ + S  T   +
Sbjct: 247 IHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITI 306

Query: 262 AFVFG-SALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 264
             +FG  A  L   +  T  WYL KFE++P +  AI P+V+
Sbjct: 307 WLLFGFVAANLTYAAGETHRWYLRKFENYPANRHAIFPYVY 343

The following BLAST results are available for this feature:

BLAST of Cla97C05G103790 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446721.1	1.7e-114	86.43	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo]	[more]
XP_004135372.1	4.4e-110	82.56	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis sativus] >KGN52157.1 hyp...	[more]
XP_022985155.1	4.7e-104	76.15	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima]	[more]
XP_023552351.1	5.8e-102	76.25	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022957136.1	2.2e-101	75.10	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]

BLAST of Cla97C05G103790 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BFS4\|A0A1S3BFS4_CUCME	1.1e-114	86.43	steroid 5-alpha-reductase DET2-like OS=Cucumis melo OX=3656 GN=LOC103489358 PE=4...	[more]
tr\|A0A0A0KUP2\|A0A0A0KUP2_CUCSA	2.9e-110	82.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G612860 PE=4 SV=1	[more]
tr\|A0A2I4H7C8\|A0A2I4H7C8_9ROSI	3.0e-70	53.56	steroid 5-alpha-reductase DET2-like OS=Juglans regia OX=51240 GN=LOC109014156 PE...	[more]
tr\|A0A2I4HIX5\|A0A2I4HIX5_9ROSI	2.5e-69	53.18	steroid 5-alpha-reductase DET2-like OS=Juglans regia OX=51240 GN=LOC109018421 PE...	[more]
tr\|I1JDE1\|I1JDE1_SOYBN	3.3e-69	52.67	Uncharacterized protein OS=Glycine max OX=3847 GN=100798454 PE=4 SV=1	[more]

BLAST of Cla97C05G103790 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q2QDF6\|DET2_GOSHI	1.3e-17	32.00	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
sp\|Q28891\|S5A1_MACFA	4.3e-16	28.70	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
sp\|P31213\|S5A2_HUMAN	1.6e-15	31.78	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 S...	[more]
sp\|P18405\|S5A1_HUMAN	2.8e-15	28.33	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
sp\|A5PJS2\|S5A1_BOVIN	1.4e-14	29.20	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus OX=9913 GN=SRD5A1 PE=2 SV=...	[more]

BLAST of Cla97C05G103790 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16010.1	2.6e-64	47.43	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	9.4e-14	29.44	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	9.4e-14	35.53	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	2.2e-07	27.69	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	5.0e-07	28.05	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane
GO:0005737	cytoplasm

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR039357	SRD5A/TECR
IPR001104	3-oxo-5_a-steroid_4-DH_C

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C05G103790.1	Cla97C05G103790.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 154..263 e-value: 3.3E-30 score: 104.9
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 158..227 score: 26.388
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 14..263
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 14..263

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla97C05G103790	Watermelon (Charleston Gray)	wcgwmbB229
Cla97C05G103790	Watermelon (97103) v1	wmwmbB092
Cla97C05G103790	Watermelon (97103) v1	wmwmbB207
Cla97C05G103790	Wax gourd	wgowmbB531
Cla97C05G103790	Wax gourd	wgowmbB532
Cla97C05G103790	Watermelon (97103) v2	wmbwmbB143
Cla97C05G103790	Silver-seed gourd	carwmbB0878
Cla97C05G103790	Silver-seed gourd	carwmbB1109
Cla97C05G103790	Cucumber (Gy14) v2	cgybwmbB365
Cla97C05G103790	Cucurbita maxima (Rimu)	cmawmbB423
Cla97C05G103790	Cucumber (Gy14) v1	cgywmbB498
Cla97C05G103790	Cucurbita maxima (Rimu)	cmawmbB758
Cla97C05G103790	Cucurbita moschata (Rifu)	cmowmbB403
Cla97C05G103790	Cucurbita moschata (Rifu)	cmowmbB729
Cla97C05G103790	Wild cucumber (PI 183967)	cpiwmbB402
Cla97C05G103790	Cucumber (Chinese Long) v3	cucwmbB398
Cla97C05G103790	Cucumber (Chinese Long) v2	cuwmbB383
Cla97C05G103790	Bottle gourd (USVL1VR-Ls)	lsiwmbB284
Cla97C05G103790	Melon (DHL92) v3.6.1	medwmbB056
Cla97C05G103790	Melon (DHL92) v3.5.1	mewmbB060