CSPI02G19270 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G19270
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCalcium-binding EF-hand family protein, putative
LocationChr2 : 17552741 .. 17553367 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAACACACACACTCAAAACTCACAACCCCAAACGCAATAAACAAACACGAAGCTATATCCTTTTTCCCACAAAAGAAATGGCTCTTCCTGGTGTAAGCAAGTTCAATTTCTCAAGGGAAGAGATGAAGGAAATCTTCCGTGAGCACGACATCGACGGAGACGGTTACCTCAGCATCAGTGAGCTCATCAAAGCCATGGGGTTCTTGGGTCACTCTATACCATTCTACAAAGCTCACTACGGCATGGCTTATTGTGATGAGAATGGTGACGGCCTCATCTCAGAAGACGAGCTTGACAAACTCATCGATTATGCCGAAAGGTTTCAGAAAAGAAGGCGTTGATAGAAATTAAAATTGGCAACACCCTATGATTTTATGTTTTTGTTTCCTAAGCTTTTGACTTTCACTAATAAAGGAAGGAAGGGCTGGGCTTGAGCAAGTTGGTCCTTCTTGTGTTTATGTAATCTTGTTGCGAAATGGAAAAGGCTTCTAATATCTATTATTAGTTAATATGTGGGTTTTAGGCTAATCTTATTTAAACAATCGTTACATTAAAAGCAAGCTAAAAGTTATTACTTAATTAATTACTAATAAACATTAACAATTAGATATTGATCAAATCGAG

mRNA sequence

ATGGCTCTTCCTGGTGTAAGCAAGTTCAATTTCTCAAGGGAAGAGATGAAGGAAATCTTCCGTGAGCACGACATCGACGGAGACGGTTACCTCAGCATCAGTGAGCTCATCAAAGCCATGGGGTTCTTGGGTCACTCTATACCATTCTACAAAGCTCACTACGGCATGGCTTATTGTGATGAGAATGGTGACGGCCTCATCTCAGAAGACGAGCTTGACAAACTCATCGATTATGCCGAAAGGTTTCAGAAAAGAAGGCGTTGA

Coding sequence (CDS)

ATGGCTCTTCCTGGTGTAAGCAAGTTCAATTTCTCAAGGGAAGAGATGAAGGAAATCTTCCGTGAGCACGACATCGACGGAGACGGTTACCTCAGCATCAGTGAGCTCATCAAAGCCATGGGGTTCTTGGGTCACTCTATACCATTCTACAAAGCTCACTACGGCATGGCTTATTGTGATGAGAATGGTGACGGCCTCATCTCAGAAGACGAGCTTGACAAACTCATCGATTATGCCGAAAGGTTTCAGAAAAGAAGGCGTTGA
Homology
[Close]
BLAST of CSPI02G19270 vs. Swiss-Prot
Match: CML16_ARATH (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana GN=CML16 PE=2 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 6.6e-06
Identity = 22/65 (33.85%), Postives = 40/65 (61.54%), Query Frame = 1 

Query: 12  SREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDE 71
           ++E++ E+FR  D DG+G ++ +EL  +M  +GH + + +    M   D NGDG+IS +E
Sbjct: 84  NQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVISFNE 143

Query: 72  LDKLI 77
              ++
Sbjct: 144 FSHIM 148

[Close]
BLAST of CSPI02G19270 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 9.5e-44
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1 

Query: 1  MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD 60
          MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD
Sbjct: 1  MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD 60

Query: 61 ENGDGLISEDELDKLIDYAERFQKRRR 88
          ENGDGLISEDELDKLIDYAERFQKRRR
Sbjct: 61 ENGDGLISEDELDKLIDYAERFQKRRR 87

[Close]
BLAST of CSPI02G19270 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 3.4e-25
Identity = 52/81 (64.20%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
          ++   SRE++KEIFREHDIDGDG LS++E++KA G  G  +PFYKAHYGMA+ DENGDG 
Sbjct: 6  TRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGF 65

Query: 67 ISEDELDKLIDYAERFQKRRR 88
          I E+ELDKL+DYA++FQ +R+
Sbjct: 66 IEEEELDKLVDYAQKFQHKRK 86

[Close]
BLAST of CSPI02G19270 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 6.2e-19
Identity = 45/80 (56.25%), Postives = 60/80 (75.00%), Query Frame = 1 

Query: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
           S   FSRE++KEIF+ HD D DG+L++ E+ KA  FLG  +P YKA++GM Y D N DGL
Sbjct: 97  SNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGL 156

Query: 67  ISEDELDKLIDYAERFQKRR 87
           IS+DEL+KL+DYA +F  +R
Sbjct: 157 ISKDELEKLVDYAYKFFNKR 176

[Close]
BLAST of CSPI02G19270 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 6.9e-18
Identity = 45/80 (56.25%), Postives = 58/80 (72.50%), Query Frame = 1 

Query: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
           S   FSRE++KEIF+ +D D DG+L+I E+ KA   LG   PF KA++GM Y D N DGL
Sbjct: 121 SNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYADTNKDGL 180

Query: 67  ISEDELDKLIDYAERFQKRR 87
           I EDELDKLI+YA +F K++
Sbjct: 181 IGEDELDKLINYANKFMKKK 200

[Close]
BLAST of CSPI02G19270 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 9.0e-18
Identity = 47/80 (58.75%), Postives = 56/80 (70.00%), Query Frame = 1 

Query: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
           S  + SRE +KEIF+ HD + DG+L+  EL KA  FLG   PFYKA YGM Y D N DGL
Sbjct: 92  SNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGL 151

Query: 67  ISEDELDKLIDYAERFQKRR 87
           ISE ELDKLIDYA +  K++
Sbjct: 152 ISEAELDKLIDYASKIIKKK 171

[Close]
BLAST of CSPI02G19270 vs. TAIR10
Match: AT3G25600.1 (AT3G25600.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-07
Identity = 22/65 (33.85%), Postives = 40/65 (61.54%), Query Frame = 1 

Query: 12  SREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDE 71
           ++E++ E+FR  D DG+G ++ +EL  +M  +GH + + +    M   D NGDG+IS +E
Sbjct: 84  NQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVISFNE 143

Query: 72  LDKLI 77
              ++
Sbjct: 144 FSHIM 148

[Close]
BLAST of CSPI02G19270 vs. TAIR10
Match: AT3G03000.1 (AT3G03000.1 EF hand calcium-binding protein family)

HSP 1 Score: 49.7 bits (117), Expect = 1.1e-06
Identity = 25/76 (32.89%), Postives = 42/76 (55.26%), Query Frame = 1 

Query: 4   PGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENG 63
           P + K  ++ +++K IFR  D DG+GY++ +EL  +M  LGH++   +    +   D +G
Sbjct: 83  PDLVKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEADRDG 142

Query: 64  DGLISEDELDKLIDYA 80
           DG I   E  + I  A
Sbjct: 143 DGCIDFQEFVQAITSA 158

[Close]
BLAST of CSPI02G19270 vs. TAIR10
Match: AT1G32250.1 (AT1G32250.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 47.4 bits (111), Expect = 5.4e-06
Identity = 22/69 (31.88%), Postives = 40/69 (57.97%), Query Frame = 1 

Query: 11  FSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISED 70
           ++ E++  +FR  D DG+G+++ +EL  +M  LGH++   +    +   D +GDG I+  
Sbjct: 91  YTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAELTGMIKEADSDGDGRINFQ 150

Query: 71  ELDKLIDYA 80
           E  K I+ A
Sbjct: 151 EFAKAINSA 159

[Close]
BLAST of CSPI02G19270 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 183.7 bits (465), Expect = 1.4e-43
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1 

Query: 1  MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD 60
          MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD
Sbjct: 1  MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD 60

Query: 61 ENGDGLISEDELDKLIDYAERFQKRRR 88
          ENGDGLISEDELDKLIDYAERFQKRRR
Sbjct: 61 ENGDGLISEDELDKLIDYAERFQKRRR 87

[Close]
BLAST of CSPI02G19270 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 122.1 bits (305), Expect = 4.9e-25
Identity = 52/81 (64.20%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
           ++   SRE++KEIFREHDIDGDG LS++E++KA G  G  +PFYKAHYGMA+ DENGDG 
Sbjct: 87  TRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGF 146

Query: 67  ISEDELDKLIDYAERFQKRRR 88
           I E+ELDKL+DYA++FQ +R+
Sbjct: 147 IEEEELDKLVDYAQKFQHKRK 167

[Close]
BLAST of CSPI02G19270 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 122.1 bits (305), Expect = 4.9e-25
Identity = 52/81 (64.20%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
          ++   SRE++KEIFREHDIDGDG LS++E++KA G  G  +PFYKAHYGMA+ DENGDG 
Sbjct: 6  TRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGF 65

Query: 67 ISEDELDKLIDYAERFQKRRR 88
          I E+ELDKL+DYA++FQ +R+
Sbjct: 66 IEEEELDKLVDYAQKFQHKRK 86

[Close]
BLAST of CSPI02G19270 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 101.3 bits (251), Expect = 8.9e-19
Identity = 45/80 (56.25%), Postives = 60/80 (75.00%), Query Frame = 1 

Query: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
           S   FSRE++KEIF+ HD D DG+L++ E+ KA  FLG  +P YKA++GM Y D N DGL
Sbjct: 97  SNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGL 156

Query: 67  ISEDELDKLIDYAERFQKRR 87
           IS+DEL+KL+DYA +F  +R
Sbjct: 157 ISKDELEKLVDYAYKFFNKR 176

[Close]
BLAST of CSPI02G19270 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 97.8 bits (242), Expect = 9.9e-18
Identity = 45/80 (56.25%), Postives = 58/80 (72.50%), Query Frame = 1 

Query: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66
           S   FSRE++KEIF+ +D D DG+L+I E+ KA   LG   PF KA++GM Y D N DGL
Sbjct: 121 SNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYADTNKDGL 180

Query: 67  ISEDELDKLIDYAERFQKRR 87
           I EDELDKLI+YA +F K++
Sbjct: 181 IGEDELDKLINYANKFMKKK 200

The following BLAST results are available for this feature:
BLAST of CSPI02G19270 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML16_ARATH6.6e-0633.85Probable calcium-binding protein CML16 OS=Arabidopsis thaliana GN=CML16 PE=2 SV=... [more]
BLAST of CSPI02G19270 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKX1_CUCSA9.5e-44100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1[more]
A0A0A0LRC1_CUCSA3.4e-2564.20Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1[more]
A0A0A0KIY4_CUCSA6.2e-1956.25Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1[more]
A0A0A0KLF3_CUCSA6.9e-1856.25Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1[more]
A0A0A0KMQ1_CUCSA9.0e-1858.75Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1[more]
BLAST of CSPI02G19270 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G25600.13.7e-0733.85 Calcium-binding EF-hand family protein[more]
AT3G03000.11.1e-0632.89 EF hand calcium-binding protein family[more]
AT1G32250.15.4e-0631.88 Calcium-binding EF-hand family protein[more]
BLAST of CSPI02G19270 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700207406|gb|KGN62525.1|1.4e-43100.00hypothetical protein Csa_2G359940 [Cucumis sativus][more]
gi|449470198|ref|XP_004152805.1|4.9e-2564.20PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus][more]
gi|700207397|gb|KGN62516.1|4.9e-2564.20hypothetical protein Csa_2G358870 [Cucumis sativus][more]
gi|700194513|gb|KGN49690.1|8.9e-1956.25hypothetical protein Csa_5G068170 [Cucumis sativus][more]
gi|700194512|gb|KGN49689.1|9.9e-1856.25hypothetical protein Csa_5G067670 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G19270.1CSPI02G19270.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 15..40
score: 5.
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 60..75
score: 0
IPR002048EF-hand domainSMARTSM00054efh_1coord: 15..43
score: 3.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 11..46
score: 15.183coord: 60..82
score: 7
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 12..76
score: 4.7
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 7..75
score: 3.71
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 60..72
score: -coord: 24..36
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 12..79
score: 7.5
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 12..79
score: 7.5

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G19270MELO3C010876Melon (DHL92) v3.5.1cpimeB118
CSPI02G19270CsaV3_2G027960Cucumber (Chinese Long) v3cpicucB081
CSPI02G19270Bhi04G000306Wax gourdcpiwgoB159
CSPI02G19270Carg08773Silver-seed gourdcarcpiB0115
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G19270Cucurbita pepo (Zucchini)cpecpiB092
CSPI02G19270Cucurbita pepo (Zucchini)cpecpiB541
CSPI02G19270Cucurbita pepo (Zucchini)cpecpiB773
CSPI02G19270Cucurbita pepo (Zucchini)cpecpiB820
CSPI02G19270Bottle gourd (USVL1VR-Ls)cpilsiB114
CSPI02G19270Bottle gourd (USVL1VR-Ls)cpilsiB141
CSPI02G19270Melon (DHL92) v3.6.1cpimedB100
CSPI02G19270Melon (DHL92) v3.6.1cpimedB114
CSPI02G19270Melon (DHL92) v3.6.1cpimedB142
CSPI02G19270Cucumber (Gy14) v2cgybcpiB057
CSPI02G19270Cucumber (Gy14) v2cgybcpiB163
CSPI02G19270Cucumber (Gy14) v2cgybcpiB305
CSPI02G19270Silver-seed gourdcarcpiB0209
CSPI02G19270Silver-seed gourdcarcpiB0806
CSPI02G19270Cucumber (Chinese Long) v3cpicucB103
CSPI02G19270Watermelon (97103) v2cpiwmbB137
CSPI02G19270Watermelon (97103) v2cpiwmbB166
CSPI02G19270Wax gourdcpiwgoB198
CSPI02G19270Wild cucumber (PI 183967)cpicpiB074
CSPI02G19270Wild cucumber (PI 183967)cpicpiB091
CSPI02G19270Cucumber (Gy14) v1cgycpiB163
CSPI02G19270Cucumber (Gy14) v1cgycpiB331
CSPI02G19270Cucumber (Gy14) v1cgycpiB341
CSPI02G19270Cucurbita maxima (Rimu)cmacpiB011
CSPI02G19270Cucurbita maxima (Rimu)cmacpiB175
CSPI02G19270Cucurbita maxima (Rimu)cmacpiB439
CSPI02G19270Cucurbita maxima (Rimu)cmacpiB513
CSPI02G19270Cucurbita maxima (Rimu)cmacpiB793
CSPI02G19270Cucurbita moschata (Rifu)cmocpiB002
CSPI02G19270Cucurbita moschata (Rifu)cmocpiB160
CSPI02G19270Cucurbita moschata (Rifu)cmocpiB428
CSPI02G19270Cucurbita moschata (Rifu)cmocpiB782
CSPI02G19270Cucumber (Chinese Long) v2cpicuB065
CSPI02G19270Cucumber (Chinese Long) v2cpicuB080
CSPI02G19270Cucumber (Chinese Long) v2cpicuB096
CSPI02G19270Melon (DHL92) v3.5.1cpimeB105
CSPI02G19270Melon (DHL92) v3.5.1cpimeB148
CSPI02G19270Watermelon (Charleston Gray)cpiwcgB136
CSPI02G19270Watermelon (Charleston Gray)cpiwcgB144
CSPI02G19270Watermelon (Charleston Gray)cpiwcgB172
CSPI02G19270Watermelon (97103) v1cpiwmB116
CSPI02G19270Watermelon (97103) v1cpiwmB129
CSPI02G19270Watermelon (97103) v1cpiwmB153