CSPI01G27140 (gene) Wild cucumber (PI 183967)

NameCSPI01G27140
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionDefensin-like family protein, putative
LocationChr1 : 22079794 .. 22080776 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGTTAGGAGCAACTTTTGGTTTGGTCTTCATTGCCTTCCTCGTCTTATACTCAGGTCGATCTCTCTTTACTTACTCTCTTTCTCGATTAAGTTTTCAATGATTGTTTTTAATAAAATTACTTTTTAGATGACAATCTTTTCACAAGAAATTGCAATTCATAACAAAGTTAAGAGTACCATCAAACTTACGGTAAATCACAACTACGTTATATTATATTTTCTTAATGCTTTTTAAGTGCAACGCTCGAGTTTAGGAGAAGGACGTGAATGAACCGATATTATTACATCGAAATGAAACTGATCATGAGGACATGAAAGTAAAATTAATAAAGACTTTTAAAAAAAATTAAAAGTTGCTACGTATTCCAACGTGCACTTCGGTGAGTTCAATCATAGGAACTCTAAAATTAAGCATGTTTGGGCTTAAAACATTCCTATGTTTGGGTGATCTCTCAAGAAATTTTTCTAGGAAAAAGATGATGTTGCTAGGTCGCAAGATATGCATGAAAATGTTAAGGATCCAAATTCCAAATCCTGGTTTGAATTCTAGGACGAGAGTGTTACATATATGAGTGTTACATTAAGGGTTTTGTTTTTTTTTTTTAATCTTTTATCTAAAAATATTTATAAATATAACAAATTATCACAATCTATATGTGATATACATACACCACAATACGCTACTTATCTATGTATGTCTATCATGATAAAATAGATACATATACTAGTTTATCGAAGTTGTCTTGATTTGAAAATAACGAAACCCTAAAATGAACGGATCGTTGATATAATTACAGGGAGTATGAATACAAATGCACAATTGTGTTGCAACAATCATCCAGAGTTGGGAGAATGCGAGGCAGGAGTAGACGATGCAAATGATGGGAAGTGTTGGCTTCATTGCATTGCTGGTTGTGAGAAAGGTGGATTCTGTAAACATCTCAGTGATAAAAATGTCTGTCATTGTTATTGTTGA

mRNA sequence

ATGGCGAAGTTAGGAGCAACTTTTGGTTTGGTCTTCATTGCCTTCCTCGTCTTATACTCAGGGAGTATGAATACAAATGCACAATTGTGTTGCAACAATCATCCAGAGTTGGGAGAATGCGAGGCAGGAGTAGACGATGCAAATGATGGGAAGTGTTGGCTTCATTGCATTGCTGGTTGTGAGAAAGGTGGATTCTGTAAACATCTCAGTGATAAAAATGTCTGTCATTGTTATTGTTGA

Coding sequence (CDS)

ATGGCGAAGTTAGGAGCAACTTTTGGTTTGGTCTTCATTGCCTTCCTCGTCTTATACTCAGGGAGTATGAATACAAATGCACAATTGTGTTGCAACAATCATCCAGAGTTGGGAGAATGCGAGGCAGGAGTAGACGATGCAAATGATGGGAAGTGTTGGCTTCATTGCATTGCTGGTTGTGAGAAAGGTGGATTCTGTAAACATCTCAGTGATAAAAATGTCTGTCATTGTTATTGTTGA
BLAST of CSPI01G27140 vs. Swiss-Prot
Match: DEF20_ARATH (Putative defensin-like protein 20 OS=Arabidopsis thaliana GN=At5g52605 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 7.8e-14
Identity = 29/53 (54.72%), Postives = 34/53 (64.15%), Query Frame = 1

Query: 29 LCCNNHPELGECEAGVDD--ANDGKCWLHCIAGCEKGGFCKHLSDKNVCHCYC 80
          +CC  HP +G C  G+DD    DGKCW  CI GCE GGFCK    K++CHC C
Sbjct: 36 ICCKEHPSVGRCLPGIDDDAEKDGKCWKFCIEGCETGGFCKLFEHKHICHCNC 88

BLAST of CSPI01G27140 vs. Swiss-Prot
Match: DEF22_ARATH (Defensin-like protein 22 OS=Arabidopsis thaliana GN=At5g08315 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.0e-10
Identity = 26/60 (43.33%), Postives = 35/60 (58.33%), Query Frame = 1

Query: 22 SMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCE--KGGFCKHLSDKNVCHCYC 80
          ++     LCCNNHP+ G+C    DD    +C   C+ GC+  KGG+CK +S    CHCYC
Sbjct: 26 NVENGGSLCCNNHPKFGKCNTKNDDQ---RCNSWCLNGCDNGKGGYCKSMSHGGQCHCYC 82

BLAST of CSPI01G27140 vs. Swiss-Prot
Match: DEF25_ARATH (Putative defensin-like protein 25 OS=Arabidopsis thaliana GN=At5g08055 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.7e-08
Identity = 28/75 (37.33%), Postives = 41/75 (54.67%), Query Frame = 1

Query: 7  TFGLVFI-AFLVLYSG-SMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCEKGG 66
          +F L+ +  F V   G ++ +   LCCNNHP+ G+C    D+    + W H   G  KG 
Sbjct: 8  SFALILVLTFSVDVEGYNVESGGSLCCNNHPKFGKCNTNNDNQRCNR-WCHNGCGNGKGD 67

Query: 67 FCKHLSDKNVCHCYC 80
          +CK +S   +CHCYC
Sbjct: 68 YCKAMSHGGLCHCYC 81

BLAST of CSPI01G27140 vs. Swiss-Prot
Match: DEF26_ARATH (Putative defensin-like protein 26 OS=Arabidopsis thaliana GN=At1g33607 PE=5 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.9e-06
Identity = 26/73 (35.62%), Postives = 37/73 (50.68%), Query Frame = 1

Query: 7  TFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCEKGGFC 66
          TF ++ + + V   GS      LCCN+HP+ G+C    D+    + W H   G  KGG+ 
Sbjct: 16 TFSVIGMIYNVESGGS------LCCNSHPKFGKCNTNNDEQRCNR-WCHNGCGNGKGGYY 75

Query: 67 KHLSDKNVCHCYC 80
          K +S    CH YC
Sbjct: 76 KSMSHGGQCHYYC 81

BLAST of CSPI01G27140 vs. TrEMBL
Match: A0A0A0M1N0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573060 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 7.3e-43
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60
          MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60

Query: 61 EKGGFCKHLSDKNVCHCYC 80
          EKGGFCKHLSDKNVCHCYC
Sbjct: 61 EKGGFCKHLSDKNVCHCYC 79

BLAST of CSPI01G27140 vs. TrEMBL
Match: A0A0A0LWJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573030 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 9.3e-30
Identity = 60/79 (75.95%), Postives = 64/79 (81.01%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60
          MAKLGA F  VF+AFLVLY+GSMNTNAQLCCNNH ELG C  GVDD  DGKCW HCI GC
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDDDYDGKCWHHCIVGC 60

Query: 61 EKGGFCKHLSDKNVCHCYC 80
          E+GGFCK L   +VCHCYC
Sbjct: 61 ERGGFCKKLWYGHVCHCYC 79

BLAST of CSPI01G27140 vs. TrEMBL
Match: A0A0A0LYL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573040 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-27
Identity = 54/79 (68.35%), Postives = 61/79 (77.22%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60
          MAK+G    +VFI FL  YSGS+NTNAQLCC NHPE G C+ GVDDA DG+CWL CI GC
Sbjct: 1  MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDDATDGRCWLKCIGGC 60

Query: 61 EKGGFCKHLSDKNVCHCYC 80
          EKGG CK +  K+VCHCYC
Sbjct: 61 EKGGICKRVGKKHVCHCYC 79

BLAST of CSPI01G27140 vs. TrEMBL
Match: A0A0A0LZ58_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573070 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 5.7e-19
Identity = 45/83 (54.22%), Postives = 59/83 (71.08%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDA--NDGKCWLHCIA 60
          MAK+G     V + FLV YSG++N NA +CCNN+P LG C+ GVDD+   +GKCW  C +
Sbjct: 1  MAKVGL-ISCVLVVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60

Query: 61 GCEKGGFCKHL--SDKNVCHCYC 80
          GC KGGFCK +  + ++VCHCYC
Sbjct: 61 GCTKGGFCKRVGRNGRHVCHCYC 82

BLAST of CSPI01G27140 vs. TrEMBL
Match: A0A0A0LW61_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573050 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-15
Identity = 35/67 (52.24%), Postives = 50/67 (74.63%), Query Frame = 1

Query: 17 VLYSGSMNTNAQLCCNNHPELGECEAGVDDA--NDGKCWLHCIAGCEKGGFCKHL--SDK 76
          ++ +G++N NA +CCNN+P LG C+ GVDD+   +GKCW  C +GC KGGFCK +  + +
Sbjct: 1  MMITGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGR 60

Query: 77 NVCHCYC 80
          +VCHCYC
Sbjct: 61 HVCHCYC 67

BLAST of CSPI01G27140 vs. TAIR10
Match: AT5G52605.1 (AT5G52605.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 77.4 bits (189), Expect = 4.4e-15
Identity = 29/53 (54.72%), Postives = 34/53 (64.15%), Query Frame = 1

Query: 29 LCCNNHPELGECEAGVDD--ANDGKCWLHCIAGCEKGGFCKHLSDKNVCHCYC 80
          +CC  HP +G C  G+DD    DGKCW  CI GCE GGFCK    K++CHC C
Sbjct: 36 ICCKEHPSVGRCLPGIDDDAEKDGKCWKFCIEGCETGGFCKLFEHKHICHCNC 88

BLAST of CSPI01G27140 vs. TAIR10
Match: AT5G08315.1 (AT5G08315.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 63.9 bits (154), Expect = 5.1e-11
Identity = 26/60 (43.33%), Postives = 35/60 (58.33%), Query Frame = 1

Query: 22 SMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCE--KGGFCKHLSDKNVCHCYC 80
          ++     LCCNNHP+ G+C    DD    +C   C+ GC+  KGG+CK +S    CHCYC
Sbjct: 26 NVENGGSLCCNNHPKFGKCNTKNDDQ---RCNSWCLNGCDNGKGGYCKSMSHGGQCHCYC 82

BLAST of CSPI01G27140 vs. TAIR10
Match: AT5G08055.1 (AT5G08055.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 59.7 bits (143), Expect = 9.5e-10
Identity = 28/75 (37.33%), Postives = 41/75 (54.67%), Query Frame = 1

Query: 7  TFGLVFI-AFLVLYSG-SMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCEKGG 66
          +F L+ +  F V   G ++ +   LCCNNHP+ G+C    D+    + W H   G  KG 
Sbjct: 8  SFALILVLTFSVDVEGYNVESGGSLCCNNHPKFGKCNTNNDNQRCNR-WCHNGCGNGKGD 67

Query: 67 FCKHLSDKNVCHCYC 80
          +CK +S   +CHCYC
Sbjct: 68 YCKAMSHGGLCHCYC 81

BLAST of CSPI01G27140 vs. TAIR10
Match: AT1G33607.1 (AT1G33607.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 50.8 bits (120), Expect = 4.4e-07
Identity = 26/73 (35.62%), Postives = 37/73 (50.68%), Query Frame = 1

Query: 7  TFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCEKGGFC 66
          TF ++ + + V   GS      LCCN+HP+ G+C    D+    + W H   G  KGG+ 
Sbjct: 16 TFSVIGMIYNVESGGS------LCCNSHPKFGKCNTNNDEQRCNR-WCHNGCGNGKGGYY 75

Query: 67 KHLSDKNVCHCYC 80
          K +S    CH YC
Sbjct: 76 KSMSHGGQCHYYC 81

BLAST of CSPI01G27140 vs. NCBI nr
Match: gi|700211040|gb|KGN66136.1| (hypothetical protein Csa_1G573060 [Cucumis sativus])

HSP 1 Score: 180.6 bits (457), Expect = 1.1e-42
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60
          MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60

Query: 61 EKGGFCKHLSDKNVCHCYC 80
          EKGGFCKHLSDKNVCHCYC
Sbjct: 61 EKGGFCKHLSDKNVCHCYC 79

BLAST of CSPI01G27140 vs. NCBI nr
Match: gi|700211037|gb|KGN66133.1| (hypothetical protein Csa_1G573030 [Cucumis sativus])

HSP 1 Score: 137.1 bits (344), Expect = 1.3e-29
Identity = 60/79 (75.95%), Postives = 64/79 (81.01%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60
          MAKLGA F  VF+AFLVLY+GSMNTNAQLCCNNH ELG C  GVDD  DGKCW HCI GC
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDDDYDGKCWHHCIVGC 60

Query: 61 EKGGFCKHLSDKNVCHCYC 80
          E+GGFCK L   +VCHCYC
Sbjct: 61 ERGGFCKKLWYGHVCHCYC 79

BLAST of CSPI01G27140 vs. NCBI nr
Match: gi|700211038|gb|KGN66134.1| (hypothetical protein Csa_1G573040 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-27
Identity = 54/79 (68.35%), Postives = 61/79 (77.22%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGC 60
          MAK+G    +VFI FL  YSGS+NTNAQLCC NHPE G C+ GVDDA DG+CWL CI GC
Sbjct: 1  MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDDATDGRCWLKCIGGC 60

Query: 61 EKGGFCKHLSDKNVCHCYC 80
          EKGG CK +  K+VCHCYC
Sbjct: 61 EKGGICKRVGKKHVCHCYC 79

BLAST of CSPI01G27140 vs. NCBI nr
Match: gi|659100545|ref|XP_008451146.1| (PREDICTED: defensin-like protein 22 [Cucumis melo])

HSP 1 Score: 106.7 bits (265), Expect = 1.9e-20
Identity = 42/58 (72.41%), Postives = 46/58 (79.31%), Query Frame = 1

Query: 22  SMNTNAQLCCNNHPELGECEAGVDDANDGKCWLHCIAGCEKGGFCKHLSDKNVCHCYC 80
           S+NTNAQLCC NHPE G C  GVDDA DG+CW  CI GCEKGGFCK +  K+VCHCYC
Sbjct: 49  SLNTNAQLCCINHPEWGACWPGVDDATDGRCWRRCIGGCEKGGFCKRVGRKHVCHCYC 106

BLAST of CSPI01G27140 vs. NCBI nr
Match: gi|700211041|gb|KGN66137.1| (hypothetical protein Csa_1G573070 [Cucumis sativus])

HSP 1 Score: 101.3 bits (251), Expect = 8.1e-19
Identity = 45/83 (54.22%), Postives = 59/83 (71.08%), Query Frame = 1

Query: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDA--NDGKCWLHCIA 60
          MAK+G     V + FLV YSG++N NA +CCNN+P LG C+ GVDD+   +GKCW  C +
Sbjct: 1  MAKVGL-ISCVLVVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60

Query: 61 GCEKGGFCKHL--SDKNVCHCYC 80
          GC KGGFCK +  + ++VCHCYC
Sbjct: 61 GCTKGGFCKRVGRNGRHVCHCYC 82

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DEF20_ARATH7.8e-1454.72Putative defensin-like protein 20 OS=Arabidopsis thaliana GN=At5g52605 PE=3 SV=1[more]
DEF22_ARATH9.0e-1043.33Defensin-like protein 22 OS=Arabidopsis thaliana GN=At5g08315 PE=2 SV=1[more]
DEF25_ARATH1.7e-0837.33Putative defensin-like protein 25 OS=Arabidopsis thaliana GN=At5g08055 PE=3 SV=1[more]
DEF26_ARATH7.9e-0635.62Putative defensin-like protein 26 OS=Arabidopsis thaliana GN=At1g33607 PE=5 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M1N0_CUCSA7.3e-43100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573060 PE=4 SV=1[more]
A0A0A0LWJ9_CUCSA9.3e-3075.95Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573030 PE=4 SV=1[more]
A0A0A0LYL5_CUCSA1.5e-2768.35Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573040 PE=4 SV=1[more]
A0A0A0LZ58_CUCSA5.7e-1954.22Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573070 PE=4 SV=1[more]
A0A0A0LW61_CUCSA3.8e-1552.24Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573050 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52605.14.4e-1554.72 Defensin-like (DEFL) family protein[more]
AT5G08315.15.1e-1143.33 Defensin-like (DEFL) family protein[more]
AT5G08055.19.5e-1037.33 Defensin-like (DEFL) family protein[more]
AT1G33607.14.4e-0735.62 Defensin-like (DEFL) family protein[more]
Match NameE-valueIdentityDescription
gi|700211040|gb|KGN66136.1|1.1e-42100.00hypothetical protein Csa_1G573060 [Cucumis sativus][more]
gi|700211037|gb|KGN66133.1|1.3e-2975.95hypothetical protein Csa_1G573030 [Cucumis sativus][more]
gi|700211038|gb|KGN66134.1|2.1e-2768.35hypothetical protein Csa_1G573040 [Cucumis sativus][more]
gi|659100545|ref|XP_008451146.1|1.9e-2072.41PREDICTED: defensin-like protein 22 [Cucumis melo][more]
gi|700211041|gb|KGN66137.1|8.1e-1954.22hypothetical protein Csa_1G573070 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR022618Defensin-like
Vocabulary: Cellular Component
TermDefinition
GO:0005576extracellular region
Vocabulary: Biological Process
TermDefinition
GO:0050832defense response to fungus
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050832 defense response to fungus
cellular_component GO:0005576 extracellular region
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G27140.1CSPI01G27140.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022618Defensin-like proteinPFAMPF10868Defensin_likecoord: 29..79
score: 1.4
NoneNo IPR availablePANTHERPTHR34453FAMILY NOT NAMEDcoord: 2..79
score: 1.3
NoneNo IPR availablePANTHERPTHR34453:SF1DEFENSIN-LIKE (DEFL) FAMILY PROTEIN-RELATEDcoord: 2..79
score: 1.3

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None